High-Performance Liquid Chromatographic Analysis of Drugs of Abuse in Biologic Samples

Nakashima, Kenichiro

doi:10.1248/jhs.51.272

Cited by 34 publications

(14 citation statements)

References 30 publications

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“…Difficulties may arise when only aged or haemolysed blood is available. Other disadvantages are the invasive way of sampling 2 and the difficulties encountered in some countries from the legal point of view in obtaining abusers' blood samples 3 .…”

Section: Introductionmentioning

confidence: 99%

Relationship Between Oral Fluid and Blood Concentrations of Drugs of Abuse in Drivers Suspected of Driving Under the Influence of Drugs

Wille

Raes²,

Lillsunde³

et al. 2009

Therapeutic Drug Monitoring

137

113

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In recent years, the interest in the use of oral fluid as biological matrix has increased significantly, particularly for detecting driving under the influence of drugs (DUID). In this study, the relationship between the oral fluid and blood concentrations of drugs of abuse in drivers suspected of DUID is Nevertheless the data reflect the variability of the OF/B ratios in drivers under the influence of drugs.The wide range of the ratios, however, does not allow reliable calculation of the blood concentrations from oral fluid concentrations.

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Section: Introductionmentioning

confidence: 99%

Relationship Between Oral Fluid and Blood Concentrations of Drugs of Abuse in Drivers Suspected of Driving Under the Influence of Drugs

Wille

Raes²,

Lillsunde³

et al. 2009

Therapeutic Drug Monitoring

137

113

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show abstract

“…This is the most likely reason why these are widely used as recreational drugs among the young. 52 A simultaneous semi-micro column HPLC method with FL detection of MDMA, MDA, MP and AP in rat urine was examined by using DBD-F as a labeling reagent. 53 A semimicro column HPLC can reduce solvent volume and sample size by miniaturizing the method.…”

Section: ·2 Narcotics 52-59mentioning

confidence: 99%

Analytical Studies on the Development of High-Performance Liquid Chromatographic Methods with Fluorescence or Chemiluminescence Detections and Their Practical Applications

2009

Self Cite

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This review describes a comprehensive investigation focusing on syntheses of analytical reagents, followed by their utilization in development of analytical methods, and leading to practical applications for rational use of medicaments centering on abused drugs. Many kinds of analytical reagents for fluorescence and chemiluminescence detections have been synthesized with the intention of improving sensitivity. By properly combining the developed analytical reagents with an HPLC separation technique, one could determine ultra-small amounts of abused drugs such as stimulants, narcotics and obesity drugs in various matrices. Furthermore, chiral analyses of some abused drugs and evaluation of their potential for drug-drug interaction were also performed. The developed methods might be useful for forensic and toxicological studies on drug abuse. Also, the results obtained in our study might contribute to the prediction of and the protection of human health from risks of abused drugs.

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“…However, the number of simultaneous determination methods, which are simple and sensitive, for various tryptamine, phenethylamine and piperazine analogs is quite limited. HPLC-FL (Nakamura et al, 2007;Tomita et al, 2006), GC-MS (Awad et al, 2005;Theobald and Maurer 2006;Theobald et al, 2005;Lin et al, 2003;Balikova, 2005;Kikura-Hanajiri et al, 2005 and LC-MS (Leung et al, 2007;Gottardo et al, 2007;Nakashima 2005;Nishida et al, 2006;Jimenez et al, 2006;Carrera et al, 2007;Appollonio et al, 2006;Wu et al, 2008) are the most convenient methods for distinguishing the drugs. In our previous reports, fluorescence labeling methods by LC separation and TOF-MS detection were developed for the designated substances (Min et al, 2008(Min et al, , 2009).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous and group determination methods for designated substances by HPLC with multi‐channel electrochemical detection and their application to real samples

Min

Yamashita

Toyo’oka

et al. 2010

Biomedical Chromatography

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Many psychotropic substances are illegally available on the streets and/or via the Internet. This wide distribution has become a serious social problem. To control this problem, many substances have been controlled as 'designated substances' (Shitei-Yakubutsu) in Japan since April 2007 by the Pharmaceutical Affairs Law, including tryptamines, phenethylamines and piperazines. In the present study, simultaneous determination methods using HPLC with multi-channel electrochemical detection (MECD) were developed for the designated substances. The proposed methods utilizing online electrochemical oxidation are the first report on the simultaneous determination of various designated substances. The methods involve direct determination and require no complicated pretreatments such as fluorescence labeling. The designated substances were separated by reversed-phase chromatography using a TSK-gel ODS-100V (4.6 × 250 mm, i.d., 3 µm) and gradient elution by a mixture of potassium phosphate buffer, methanol and acetonitrile. The total separation of 31 designated substances was successfully performed but required long chromatographic run times. Thus, the designated substances were divided into three groups: (1) tryptamines, (2) phenethylamines and (3) piperazines and others. They were then analyzed by HPLC-MECD as another separation method. The suitable applied voltages for each designated substance were determined based upon the hydrodynamic voltammogram. The limits of detection (signal-to-noise ratio of 3) of the designated substances for the most suitable voltages were in the range of 17.1 pg (5-MeO-MIPT) to 117 ng (indan-2-amine). The calibration curves based on the peak heights were linearly related to the amounts of the designated substances (R(2)> 0.999). Good accuracy and precision by intra-day assay and inter-day assay were also obtained using the present procedures. The proposed methods were applied to the analyses of the designated substance in several real samples.

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High-Performance Liquid Chromatographic Analysis of Drugs of Abuse in Biologic Samples

Cited by 34 publications

References 30 publications

Relationship Between Oral Fluid and Blood Concentrations of Drugs of Abuse in Drivers Suspected of Driving Under the Influence of Drugs

Relationship Between Oral Fluid and Blood Concentrations of Drugs of Abuse in Drivers Suspected of Driving Under the Influence of Drugs

Analytical Studies on the Development of High-Performance Liquid Chromatographic Methods with Fluorescence or Chemiluminescence Detections and Their Practical Applications

Simultaneous and group determination methods for designated substances by HPLC with multi‐channel electrochemical detection and their application to real samples

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