High-performance liquid chromatographic analysis of roxoxacin and its N-oxide metabolite in plasma and urine

Kullberg, Max; Koss, Raymond F.; O'Neil, S K; Edelson, Jerome

doi:10.1016/s0021-9673(01)80455-6

Cited by 9 publications

(4 citation statements)

References 1 publication

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“…A number of the quinolones have been extracted from biological fluids using organic solvents, usually chloroform. The method now described relates to acrosoxacin (14). Plasma (1 ml) is acidified with 0.5 ml of 0-5 M citrate buffer (pH 5 ) and shaken with chloroform (5 ml).…”

Section: Solvent Extractionmentioning

confidence: 99%

Recent Advances in Pharmaceutical Chemistry:the 4-Quinolone Antibiotics

Jack

1986

J Clin Pharm Ther

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Section: Solvent Extractionmentioning

confidence: 99%

Recent Advances in Pharmaceutical Chemistry:the 4-Quinolone Antibiotics

Jack

1986

J Clin Pharm Ther

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“…Blood and urine were collected from a normal male volunteer after oral administration rosoxacin (4) and pipemidic acid (6). We observed that MK-366 could not be eliited from Spherisorb (silica), LiChrosorb NH2, Vydac cation exchange, and Spherisorb ODS columns (Applied Science Laboratories, State College, Pa.) under a variety of mobile phase conditions.…”

Section: Norfloxacin (Mk-366) [1-ethyl-6-fluoro-14-dihydro-4-oxo-7-(mentioning

confidence: 99%

Determination of norfloxacin, a new nalidixic acid analog, in human serum and urine by high-performance liquid chromatography

Boppana¹,

Swanson²

1982

Antimicrob Agents Chemother

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A high-performance liquid chromatographic method for the analysis of norfloxacin [1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid], a new nalidixic acid analog, in human serum and urine is described. A statistical evaluation of the assay data showed acceptable accuracy and precision for 0.1 to 10.0 ,ug of MK-366 per ml of serum and for 1.0 to 500 ,ug of MK-366 per ml of urine. MK-366 was extracted from serum and urine at pH 7.5 with methylene chloride and back-extracted with sodium hydroxide solution. Chromatography was performed on an anion-exchange column with acetonitrilephosphate buffer as the mobile phase; UV absorbance was monitored at 273 nm. The method was used to measure MK-366 in clinical specimens.boxylic acid] is a new broad-spectrum antibacterial agent that is structurally related to nalidixic acid (Fig. 1). MK-366 exhibits greater antibacterial activity against both gram-positive and gram-negative bacteria than other nalidixic acid analogs and exhibits greater activity against Pseudomonas aeruginosa than gentamicin (1,2 Extraction of samples. Serum (1 ml) or urine (200 pul) was thoroughly mixed with 100 p.l of 0.05 N NaOH (containing standards preparing the standard curve) in a 40-ml glass extraction tube. Methylene chloride (11 ml) and 0.5 M sodium phosphate buffer (0.5 ml, pH 7.5) were then added, and the tubes were gently shaken for 10 min on a mechanical shaker. After phase separation by centrifugation at 1,500 x g for 5 min, 10 ml of the methylene chloride layer was transferred to a second 40-ml extraction tube. Fresh methylene chloride (11 ml) was added to the first extraction tube, and a second extraction was performed; 10 ml of the second extract was pooled with the first organic phase.

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“…The HPLC methods were modified from previously reported procedures [4]. The HPLC systems consisted of a pump (Beckman solvent metering pump, model 1 lo), column (Varian MicroPak MCH-10,300 x 4 mm ID, with a Co-Pel1 ODs, 30-3Op precolumn), detector (Beckman variable wavelength spectrophotometer, model 155, set at 280 mm), and mobile phases (37.5% acetonitrile in 0.2 M phosphoric acid for serum analysis and 32% acetonitrile in 0.2 M phosphoric acid for urine and prostatic tissue analysis; flow rate 1.5 ml/min (1,OOO p.s.i.).…”

Section: Methodsmentioning

confidence: 99%

“…Rosoxacin concentrations in CSF were determined only by bioassay technique, whereas the HPLC procedures were applied to samples from five patients from each group, selected at random. Statistical methods employed included correlation analysis, Mann-Whitney test, Wilcoxon's test for paired differences, and Fisher's exact test [4], P values < 0.05 being considered as significant.…”

Section: Preparation Of Prostatic Tissuementioning

confidence: 99%

Human prostatic tissue concentrations of rosoxacin

et al. 1982

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The corresponding concentrations of rosoxacin in serum (S) and prostatic tissue (PT) were determined in 15 patients undergoing transurethral resection of the prostate because of benign prostatic hyperplasia. Rosoxacin concentrations were determined employing both bioassay and a high-pressure liquid chromatography procedure, with excellent correlation between the two methods. Two dosage schedules were followed, resulting in median serum concentrations of 3.6 and 5.8 microgram/ml, respectively. The ratio of PT/S was found to be fairly constant and surprisingly high, the median values being 0.46 and 0.56. These results indicate that concentrations of rosoxacin are obtained in prostatic interstitial fluid in the range of the minimal inhibitory concentrations for most of the gram-negative pathogens causing bacterial prostatitis and urinary tract infections. Acceptable drug concentrations also were determined in urine and cerebrospinal fluid. The results obtained in this study warrant further clinical trials for rosoxacin in the treatment of bacterial prostatitis and urinary infections.

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High-performance liquid chromatographic analysis of roxoxacin and its N-oxide metabolite in plasma and urine

Cited by 9 publications

References 1 publication

Recent Advances in Pharmaceutical Chemistry:the 4-Quinolone Antibiotics

Recent Advances in Pharmaceutical Chemistry:the 4-Quinolone Antibiotics

Determination of norfloxacin, a new nalidixic acid analog, in human serum and urine by high-performance liquid chromatography

Human prostatic tissue concentrations of rosoxacin

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