High-performance liquid chromatographic analysis of epinephrine enantiomers using a UV detector in series with an optical activity detector

Scott, Britt S.; Dunn, Danny L.

doi:10.1016/s0021-9673(01)90583-7

Cited by 24 publications

(4 citation statements)

References 20 publications

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“…polarimetric and circular dichroism (CD) detectors [2][3][4] , were developed [5] and are nowadays routinely used in HPLC on chiral support to identify and differentiate chiral molecules [6] . Moreover, chiroptical detectors can be applied to the determination of enantiomeric ratios on chiral stationary phases (CSPs) with baseline [7] or partial separation [8,9] , but also on achiral support [10][11][12][13] , to preparative purposes [14,15] , and to the assignment of absolute configurations [16][17][18] . They have sometimes been used to characterize complex mixtures focusing on chiral components [19] , because the analysis is simplified by hiding achiral molecules, which are not sensed by chiroptical detection.…”

Section: Introductionmentioning

confidence: 99%

Chiroptical fingerprints to characterize lavender and lavandin essential oils

Lafhal

Bombarda

Dupuy

et al. 2020

Journal of Chromatography A

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Polarimetric HPLC detector was used to acquire chromatograms for lavender/lavandin essentials oils (EOs) on different chiral stationary phases. Amylose tris-(3,5-dichloro-phenylcarbamate) immobilized on silica allowed the best separation of the numerous chiral phytomarkers and was thus used to obtain a chiroptical fingerprint for 158 samples of French lavender/lavandin EOs. Samples from different varieties (Abrial, Fine, Grosso, Maillette, Matherone, Sumian and Super) were collected over three crop years (2012, 2013 and 2014), from four different locations in France ("Alpes-de-Haute-Provence", "Ardèche", "Drôme" and "Vaucluse") to take into account seasonal and geographic origin variations. The combination of the polarimetric fingerprints and chemometrics was tested for varietal discrimination. We assessed the ability to discriminate the EOs samples and their variety by partial least squares-discriminant analysis (PLS-DA). This case study showed that liquid chromatography with polarimetric detector in tandem with chemometric analysis was efficient to differentiate the varietal origins of French lavender/lavandin EOs.

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Section: Introductionmentioning

confidence: 99%

Chiroptical fingerprints to characterize lavender and lavandin essential oils

Lafhal

Bombarda

Dupuy

et al. 2020

Journal of Chromatography A

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“…ao and -ao are optical rotatory power of d-and 1-isomer, respectively. The peak area (A(Uv)) obtained by UV detection is given by A(UK) = a2m (2) where a2 is a constant, which depends on UV absorption coefficient, analytical conditions and each type of apparatus. The ratio R, (A(PoL)/A(Uv)) can be written as the following Eq.…”

Section: Calculationmentioning

confidence: 99%

Determination of Enantiomer Ratios of d,l-Carvone in Supercritical Fluid Extracts from Caraway Seeds and Speamint Leaves by High-Performance Liquid Chromatography with Polarimetric and Ultraviolet Spectrophotometric Detection

Bounoshita¹,

Hibi²,

Nakamura

1993

ANAL. SCI.

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KeywordsEnantiomer ratio, d,l-carvone, caraway seeds, speamint leaves, polarimetric detector, supercritical fluid extractionThe determination of enantiomer ratios of optical isomers has been an important subject in pharmaceutical chemistry, biochemistry, clinical chemistry, and agricultural chemistry because the optical isomers often differ in biological activity.There are two methods for the determination of enantiomer ratios by high-performance liquid chromatography (HPLC). One employs the chromatographic enantiomer separation which can be achieved either by using a chiral stationary phase or chiral mobile phase or by derivatization into diastereomers. The other is based on the simultaneous detection of optical rotation and UV absorption. In the latter method, the apparent optical rotation representing the sum of optical rotations of d and l-type, and the UV absorption representing the total amount, are measured by polarimetric detector and UV detector, respectively. The d/ l ratio can be obtained from a calibration curve established by the response of the polarimeter and the UV detector for a known amount of d-or l-isomer. Here one assumes the response of the polarimeter becomes zero when d/1=1, whereas that of the UV detector is simply proportional to the total amount.An advantage of this method over the first one is that chromatographic separation of optical isomers is not necessary.Therefore, this method is quite useful when enantiomer separation is difficult. Thus, the strategy has been employed in the determination of enantiomer ratios of permetric acid pentafluorobenzylesterl, d,/-epinephrine2, pyrethroids3, amino acids4, and DP-1904.5 In this report, the compositional ratios of d-and 1-enantiomers of carvone in essential oils were determined by HPLC with simultaneous detection of optical rotation and UV absorption.Essential oils were extracted from caraway seeds and dry speamint leaves by supercritical fluid of carbon dioxide.Caraway seeds contain 100% d-carvone and speamint leaves contain 93% l-carvone and 7% d-carvone. Experimental ApparatusThe HPLC system consisted of a JASCO Model LC-800 series, which included an 880-PU pump, a Rheodyne 7125 injection valve, an 860-CO air circulating oven, an 870-UV variable wavelength UV detector and an 807-IT data processor. Chromatograms were recorded on a RC-250 dual-pen recorder. A DIP-181C polarimeter (JASCO) was used for polarimetric detection. A polarizes and an analyzer used in the polarimeter were made of glam-Thomson prisms. A high pressure mercury lamp was used as light source without a filter. The flow cell had a 50 mm light-path length with an internal volume of 350 µl. A supercritical fluid extraction and chromatography system, JASCO Model Super-200, was employed in the extraction for essential oils from samples. Fig. 1 Structures of carvone enantiomers.

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“…Variations concentration of NE can leads to numerous illnesses such as Parkinson's disease, Alzheimer's disease and several sclerosis hypertension. [5][6][7][8] Dissimilar approaches can be adopted for the detection of NE in pharmaceutical and biological samples such as Spectrophotometry, high performance liquid chromatography (HPLC), capillary electrophoresis, flow injection chemiluminesense, [9][10][11][12][13][14][15] thermal lens microscopy (TLM), electrochemiluminescence and fluorimeter. [16][17][18] These measures frequently have some hitches like tedious sample pre -treatment steps, being expensive and time-consuming analysis.…”

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confidence: 99%

Synthesis of Tin Doped Copper Oxide Nanocomposites for Electrochemical Sensor Application

Priya,

Mahanthesha,

Anagawadi

et al. 2024

J. Electrochem. Soc.

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Poly (Tin-doped copper oxide nanocomposites modified glassy carbon electrode) (Poly (Sn-doped CuO nanocomposite)-MGCE) made sensors were confirmed by electrochemical finding of norepinephrine (NE). The Sn-doped CuO nanocomposites were made by precipitation technique. This nanocomposite was described through scanning electron microscope and X-ray diffraction methods. To find the doped properties of the Poly (Sn-doped CuO nanocomposite)-MGCE were studied by cyclic voltammetry studies. The above studies prove that the altered electrodes have wide electroactive external area, and the reduction and oxidation peak appeared at pH 7.4 supportive electrolyte concentration 0.2 M phosphate buffer solution. Poly(Sn-doped CuO nanocomposite)-MGCE as limit of detection was low value for NE was found to be 2.22 µM and the limit of quantification values for NE was appeared as 7.41 µM, wide linear range, high sensitivity (20 to 350 µM), functional to the injection mockup analysis and the attained outcomes are acceptable, excellent reproducibility displays excessive potential in practical applications.

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High-performance liquid chromatographic analysis of epinephrine enantiomers using a UV detector in series with an optical activity detector

Cited by 24 publications

References 20 publications

Chiroptical fingerprints to characterize lavender and lavandin essential oils

Chiroptical fingerprints to characterize lavender and lavandin essential oils

Determination of Enantiomer Ratios of d,l-Carvone in Supercritical Fluid Extracts from Caraway Seeds and Speamint Leaves by High-Performance Liquid Chromatography with Polarimetric and Ultraviolet Spectrophotometric Detection

Synthesis of Tin Doped Copper Oxide Nanocomposites for Electrochemical Sensor Application

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