High-Performance Liquid Chromatographic Analysis of Saponin Compounds in Bupleurum falcatum

Park, In-Seon; Kang, Eun Mi; Kim, Namsoo

doi:10.1093/chromsci/38.6.229

Cited by 42 publications

(22 citation statements)

References 13 publications

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“…The programmed gradient elution using acetonitrile-water from 40:60 (v/v) to 50:50 (v/v) in 10 min at a flow rate of 1.0 mL/min and detection wavelength of 203 nm were used (Park et al, 2000). For the analysis of baicalin, baicalein and glycyrrhizic acid, the elution solution consisted of (A) 25 mM NaH 2 PO 4 (pH 2.5) and (B) acetonitrile.…”

Section: Analysis Of Saikosaponins Baicalin Baicalein and Glycyrrhmentioning

confidence: 99%

Ultrasound-assisted extraction methodology as a tool to improve the antioxidant properties of herbal drug Xiao-chia-hu-tang

Liu

Weng

et al. 2005

Journal of Ethnopharmacology

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Section: Analysis Of Saikosaponins Baicalin Baicalein and Glycyrrhmentioning

confidence: 99%

Ultrasound-assisted extraction methodology as a tool to improve the antioxidant properties of herbal drug Xiao-chia-hu-tang

Liu

Weng

et al. 2005

Journal of Ethnopharmacology

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“…Most studies on Bupleurum have mainly focused on component analysis, pharmacology, chemical structure, and clinical applications (Tan et al 2008; Kanazawa et al 1990; Guo et al 1996; Ebata et al 1996; Park et al 2000). Consequently, the saikosaponin biosynthetic pathway and site in Bupleurum are unknown, although some studies have reported the cloning of critical gene fragments and gene regulation in the triterpene biosynthetic pathway of Bupleurum (Kim et al 2006; Kim et al 2011; Dong et al 2011; Sui et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Immunocytochemical localization of saikosaponin-d in vegetative organs of Bupleurum scorzonerifolium Willd

Zhao

et al. 2013

Bot Stud

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BackgroundSaikosaponin-d (SSd) is an important active component of Bupleurum scorzonerifolium Willd., a traditional Chinese medicinal herb. Thus far, the biosynthetic pathway and biosynthetic site of saikosaponins in Bupleurum are largely unknown. The cellular localization of SSd will help in understanding saikosaponin biosynthesis and regulation.ResultsIn this study, we characterize for the first time the localization of SSd in B. scorzonerifolium tissues and cells using histochemistry and immunoelectron microscopy. The results show that the saikosaponin distribution in different plant organs changes as they mature. The number of SSd gold particles distinctly differed among the roots, stems, and leaves, with the particles mainly concentrated in the roots. The gold particles were mainly observed in vacuoles, with a few particles in the protoplasm; hence, SSd is mainly stored in vacuoles.ConclusionsWe speculate that saikosaponins are mainly synthesized via the mevalonate pathway in the protoplasm in young organs, and then transported to the central vacuole by the endoplasmic reticulum (ER) or the fusion of vacuoles, to protect plants from self-poisoning with the accumulation of more saikosaponins.Electronic supplementary materialThe online version of this article (doi:10.1186/1999-3110-54-32) contains supplementary material, which is available to authorized users.

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“…Based on studies on the chemical characterization of the constituent herbs of SST [17,[49][50][51][52][53], it is assumed that the S-fraction contains various classes of compounds with different polarity and structure (i.e., flavonoids, triterpenes, saccharides, amino acids). Therefore, subsequent fractionations of the S-fraction are performed based on either the polarity or the structural properties of compounds.…”

Section: Separation Of Various Classes Of Compounds From the S-fractimentioning

confidence: 99%

Separation and isolation methods for analysis of the active principles of Sho-saiko-to (SST) oriental medicine

Ohtake

Nakai

Yamamoto

et al. 2004

Journal of Chromatography B

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Sho-saiko-to (SST) was introduced into Japan as an oriental classical medicine from China approximately 1500 years ago, and it is currently the most representative Kampo medicine (traditional Japanese medicine). SST is manufactured in Japan as an ethical drug on a modern industrial scale in which the quality of ingredients is standardized with Good Manufacturing Practices (GMP) regulation. SST is widely used for the treatment of chronic hepatitis. Experimental and clinical studies including multi-center, placebo-controlled, double-blind studies have demonstrated the various pharmacological effects of SST. SST is prepared from the hot water extraction of seven raw materials, therefore many kinds of constituents are included. Three-dimensional (3D) HPLC analysis is useful for obtaining many kinds of constituents, especially low molecular ultraviolet (UV) quenching compounds, contained in SST as well as its fractions. Fingerprint pattern provided by 3D HPLC analysis makes possible to identify the overall-viewing of SST. Databases of UV spectra of the components of medicinal herbs obtained by reversed-phase (RP) HPLC using a photodiode array (PDA) and fingerprint patterns of crude drugs made by 3D HPLC analysis facilitate the identification, analysis and quality of herbal drugs. Studies using both PDA HPLC and an amino acid analysis with a fluorometric detector have found that SST contains fifteen major low molecular compounds (i.e. baicalin, wogonin-7-O-glucuronide, liquiritin, their three aglycons, liquiritin apioside, glycyrrhizin, saikosaponin b1, saikosaponin b2, ginsenoside Rg1, ginsenoside Rb1, (6)-gingerol, (6)-shogaol and arginine). These compounds have various pharmacological actions, and are assumed to be responsible, at least partly, for the pharmacological effects of SST. Although there have only been a few investigations on high molecular compounds with pharmacological actions contained in SST, several kinds of polysaccharides have been isolated from constituent herbs of SST. This review paper summarizes analytical methods of separation, isolation and identification of compounds with biological activities from SST, which is a mixture drug of medicinal herbs. Accordingly, this paper would not focus on methods of separation, isolation and analysis of particular compounds from each constituent herb of SST.

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High-Performance Liquid Chromatographic Analysis of Saponin Compounds in Bupleurum falcatum

Cited by 42 publications

References 13 publications

Ultrasound-assisted extraction methodology as a tool to improve the antioxidant properties of herbal drug Xiao-chia-hu-tang

Ultrasound-assisted extraction methodology as a tool to improve the antioxidant properties of herbal drug Xiao-chia-hu-tang

Immunocytochemical localization of saikosaponin-d in vegetative organs of Bupleurum scorzonerifolium Willd

Separation and isolation methods for analysis of the active principles of Sho-saiko-to (SST) oriental medicine

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