High-Performance Liquid Chromatographic Analysis of Steroid Hormones

Erkoç, Fıgen; Özsar, Semin; Güven, Bülent; Kalkandelen, G.; Uğrar, Ergül

doi:10.1093/chromsci/27.2.86

Cited by 12 publications

(4 citation statements)

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“…HPLC analysis of testosterone was performed using Agilent 1290 infinity LC (Agilent Technology, Waldbronn, Germany). The analytical method was modified from Refs [ 40 ]. Samples were detected using an ultraviolet-visible (UV-Vis) spectroscopy detector, and the wavelength was set to 254 nm.…”

Section: Methodsmentioning

confidence: 99%

Effect of Application Amounts on In Vitro Dermal Absorption Test Using Caffeine and Testosterone

Kim

Lee

et al. 2021

Pharmaceutics

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Dermal absorption of chemicals is a key factor in risk assessment. This study investigated the effects of different amounts of application on dermal absorption and suggested an appropriate application dose for proper dermal absorption. Caffeine and testosterone were chosen as test compounds. An in vitro dermal absorption test was performed using a Franz diffusion cell. Different amounts (5, 10, 25, and 50 mg (or µL)/cm2) of semisolid (cream) and liquid (solution) formulations containing 1% caffeine and 0.1% testosterone were applied to rat and minipig (Micropig®) skins. After 24 h, the concentrations of both compounds remaining on the skin surface and in the stratum corneum, dermis and epidermis, and receptor fluid were determined using LC-MS / MS or HPLC. Dermal absorption of both compounds decreased with increasing amounts of application in both skin types (rat and minipig) and formulations (cream and solution). Especially, dermal absorptions (%) of both compounds at 50 mg (or µL)/cm2 was significantly lower compared to 5 or 10 mg (or µL)/cm2 in both rat and minipig skins. Therefore, a low dose (5 or 10 mg (or µL)/cm2) of the formulation should be applied to obtain conservative dermal absorption.

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Section: Methodsmentioning

confidence: 99%

Effect of Application Amounts on In Vitro Dermal Absorption Test Using Caffeine and Testosterone

Kim

Lee

et al. 2021

Pharmaceutics

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“…[3H]El produced in two separate assays from diencephalic cultures was also tested with authentic radio-inert El (Steraloids) using high-performance liquid chromatography (HPLC) (Erkoc et al, 1989;Edmond et al, 1991). The radiolabeled steroid product and unlabeled steroid were dissolved in 50% acetonitrile and applied to the HPLC column [AXXI-CHROM C-18 (ODs); 5 pm particle size; RP-18 Newguard precolumn] at 34°C.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Aromatase and 5β‐reductase activity in cultures of developing zebra finch brain: An investigation of sex and regional differences

1995

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Estrogen treatment of hatchling female zebra finches causes the masculine development of singing behavior and of the telencephalic brain regions involved in the control of song. However, early estrogen treatment of males also blocks masculine development of copulatory behavior, presumably controlled by diencephalic regions. In an effort to determine whether the differences in estrogen action are related to sex and regional differences in androgen metabolism (estrogen synthesis or androgen inactivation), we measured aromatase and 5 beta-reductase activity in dissociated-cell cultures made separately from the telencephalon, diencephalon, and also cerebellum of hatchling zebra finches under a variety of conditions. Cultures from all three brain regions express high levels of aromatase and 5 beta-reductase activity. Comparisons between telencephalic and diencephalic cultures of the activity and kinetics of aromatase suggest that the telencephalic cultures convert androgen to estrogen more efficiently than diencephalic cultures, which might be important in the differential action of estrogen in the two brain regions. However, the activity of neither aromatase nor 5 beta-reductase was significantly different between the sexes in either telencephalic or diencephalic cultures. Thus, comparisons between the sexes do not support the idea that differences in posthatching aromatase or 5 beta-reductase activity account for the pattern of sexual differentiation of the song and copulatory systems.

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“…After completion of incubation, the tissue samples were carefully rinsed and lyophilized. The tissue drug content was determined through extraction either by a F‐3010 Fluorescence spectrophotometer (Hitachi Ltd., Tokyo, Japan) for carboxyfluorescein (excitation 488 nm; emission 516 nm) and RH123 (excitation 498 nm; emission 525 nm) or by high performance liquid chromatography for testosterone10 and tacrolimus 11. Experiments in the presence of any inhibitor were performed similarly.…”

Section: Methodsmentioning

confidence: 99%

Decreased Drug Penetration in Inflamed Tissue Related to Changed Mucosal Metabolism in Experimental Colitis

Pellequer

Weissenborn

Lamprecht

2007

Journal of Pharmaceutical Sciences

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High-Performance Liquid Chromatographic Analysis of Steroid Hormones

Cited by 12 publications

References 0 publications

Effect of Application Amounts on In Vitro Dermal Absorption Test Using Caffeine and Testosterone

Effect of Application Amounts on In Vitro Dermal Absorption Test Using Caffeine and Testosterone

Aromatase and 5β‐reductase activity in cultures of developing zebra finch brain: An investigation of sex and regional differences

Decreased Drug Penetration in Inflamed Tissue Related to Changed Mucosal Metabolism in Experimental Colitis

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