High-performance liquid chromatographic analysis of indapamide (RHC 2555) in urine, plasma and blood

Liquid chromatography–tandem mass spectrometry validated method for the estimation of indapamide in human whole blood

“…The methods reported in literature for estimation of indapamide in whole blood [6,[8][9][10][11][12], serum [8,13,14], plasma [11,12], and urine [11,[15][16][17][18][19] involves use of HPLC, or in combination with MS, i.e. LC-MS/MS analytical techniques.…”

“…These methods had LLOQ between 1.0 and 50.0 ng/ml, applicable for 2.5 mg, 5.0 mg of immediate release and 1.5 mg of sustainedrelease formulations. Many of these methods [8][9][10][11]14], however, do not meet some of the modern drug-development needs with respect to efficient extraction procedure, throughput or sufficient sensitivity. The capability of a single analytical method that encompasses all of the above merits to monitor the total absorption, distribution and elimination pharmacokinetic profile of the pre-clinical and clinical pharmacokinetic studies especially with low-dose sustained-release formulations is of prime concern.…”

Liquid chromatography–electrospray tandem mass spectrometry method for determination of indapamide in serum for single/multiple dose bioequivalence studies of sustained release formulations

“…Although the assay of indapamide in pharmaceutical formulations and determination of the related substances profile is frequently referred in literature, using spectrometric [1][2][3][4], electrometric [5,6] and chromatographic [7][8][9] methods, only very few HPLC/UV methods were dedicated to its determination in biological fluids [10][11][12][13][14][15].…”

“…More often, liquid-liquid extraction procedures require two or three successive steps (extraction, backextraction, and re-extraction) [12,15] to eliminate matrix interferences. More over, ethyl acetate and diethyl ether were used as extracting phases.…”

Determination of lauroyl-indapamide in rat whole blood by high-performance liquid chromatography

“…It belongs to a well-known pharmacological active compound series classified as an antihypertensive agent with diuretic activity, which can significantly reduce rat blood pressure. A number of analytical methods have been reported concerning the determination of indapamide, the parent drug of lauroyl-indapamide, in dosage form [1,2] or in biological fluids including serum, plasma, urine and whole blood [3][4][5][6][7][8][9][10][11]. However, to the best of our knowledge, there is little information available in the literatures about lauroyl-indapamide, even less about its assay in the biological fluids, which made it considered necessary to develop an accurate assay to quantify it in vivo, so as to know exactly the characteristics of the substance in biological tests.…”