High Performance Liquid Chromatographic Analysis of Rotenone Formulations: Collaborative Study

Abstract

A collaborative study was conducted on a high performance liquid chromatographic (HPLC) method for determining rotenone in formulations. One liquid and 4 dust samples, containing other rotenoids and pesticides, with rotenone guarantees ranging from 0.75% to 20% were analyzed by 16 laboratories. Peak height measurements were used by 14 laboratories while 8 laboratories used integrator area measurements. Samples were extracted on a rotary shaker for 1% h with dioxane. An aliquot was analyzed using reverse phase HPLC and UV detection at 280 nm. Coefficients of variation ranged from 2.47 to 3.19% for peak height determinations and from 2.56 to 6.00% for peak area determinations. One collaborator analyzed the samples by the official infrared method for a comparison. The HPLC method has been adopted official first action.

Determination of Whole-Body Rotenone Residues in the Brown Tree Snake (Boiga irregularis)

“…Due to its comparative lack of volatility and thermal instability, the majority of analytical methods developed for rotenone have employed high-performance liquid chromatography (HPLC) as a separation technique. Most early methods involved formulation analysis (Bushway and Hanks, 1977;Bushway, 1983Bushway, , 1984. Other techniques focused on rotenone determination in more complex tissue samples and the need for decreased limits of detection and extraction/sample cleanup procedures.…”