High performance liquid chromatographic analysis of some flavonoids of Ballota species

Abstract

547.972Ballota L. is a plant belonging to the Lamiaceae family and is represented by 16 taxa in Turkey [1]. Ballota species have been used in Turkish folk medicine as an antiulcer, antispasmodic, diuretic, choleretic, antihaemorrhoidal, and sedative agents [2,3]. The antimicrobial activities [4] and the antioxidant activities [5] of all Ballota species growing in Turkey was recently reported as well as the antifungal activities of some flavonoids isolated from B. glandulossima [6] and B. inaequidens [7]. The water extract of B. glandulosissima has been reported to have antinociceptive [8], antiinflammatory, and hepatoprotective activities [9].Previous investigations of the aerial parts of the Ballota species resulted in the isolation of some flavonoids apigenin and apigenin-7-glucoside [10,11]; kumatakenin, ladanein [10]; genkwanin, nuchensin, isokaempferide, luteolin, luteolin-7-rutinoside, quercetin-3-glycoside, salvigenin, apigenin 6,8-di-C-glycoside [10]; luteolin-7-glycoside [10]; scutallerein-7,4′-dimethylether, acacetin-7-glycoside, chrysoeriol-7-glycoside [10], luteolin-7-lactate, and luteolin-7-glycosyl lactate [12].In our previous studies, three diterpenoids (hispanolone, ballonigrine, dehydrohispanolone) and ten flavonoids (kumatakenin, pachypodol, 5-hydroxy-7,3′,4′-trimethoxyflavone, velutin, corymbosine, 5-hydroxy-3,7,4′-trimethoxyflavone, retusin, 5-hydroxy-7,4′-dimethoxyflavone, 5-hydroxy-3,6,7,4′-tetramethoxyflavone, ladanein) were isolated, chemically characterized, and analyzed by HPLC in different species of Ballota [6,7,13,14].Thus, as a continuation of our research [3-9, 13-15] on this genus we now report on the flavonoids present in the acetone extracts from sixteen species of the genus Ballota. Our aim is to contribute to their chemotaxonomic determination and establish the flavonoid profiles of Ballota species. To our knowledge, no data are available with respect to the chemotaxonomic determination and HPLC analysis of this genus.

A Review of the Phytochemistry, Traditional Uses, and Biological Activities of the Genus Ballota and Otostegia

“…The inhibition activity was higher for the latter enzyme (IC 50 22.3, 17.5 and 14.2 µM, respectively) than for the former (IC 50 38.5, 47.2, and 103.7 µM) [112]. These enzymatic systems were also the targets of another similar work concerning the isolation of 6 clerodane tricyclic diterpenes from the chloroform extract of B. limbata [107]: ballatenolide A (59), 15-methoxypatagonic acid (71), patagonic acid (72), and limbatenolides A-C (54)(55)(56). All of the compounds showed inhibitory activity with BChE with IC 50 values ranging from 24.9 to 51.0 µM, lower than the standard inhibitor galanthamine (8.5 µM).…”

“…antalyensis, B. cristata, B. larendana, B. saxatilis subsp. brachyodonta[72,73], B. aucheri[74], B. inaequidens[72,73,75,76], B. lanata[77], B. nigra[70], B. nigra subsp. foetida[72,73,78], B. pseudodictamnus[79], B. rupestris[70,78], B. saxatilis[72,73, 80], B. undulata[81], O. fruticosa[82]…”

Pharmacological activities of Ballota acetabulosa (L.) Benth: A mini review

“…Labdane diterpenes Ballonigrin, dehydrohispanolone (hispanone) 11,12 18-hydroxyballonigrin 13 Flavones Apigenin 14,15 Ladanein 12…”

Chemical Composition and Antimicrobial Activity of Essential Oils of Ballota nigra Subsp. kurdica From Iran

“…It has been reported that 33 to 35 species exist in the genus Ballota that are mainly distributed around the Mediterranean and Eurasia (6,7). Ballota species have been widely used in traditional medicine as sedative, antispasmodic, diuretic, choleretic, and antihemorrhoidal agents (8). Several compounds have been reported in B. nigra, including terpenes, flavonoids, phenylpropanoids, and tannins (9).…”