High Performance Liquid Chromatographic Analysis of Residues of Thiampenicol in Beef Muscle

Psomas, J. E.; Iosifidoy, E. G.

doi:10.1080/10826079308019600

Cited by 6 publications

(1 citation statement)

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“…Untill now TAP residues were often analyzed by chromatographic methods using liquid chromatography (Felice et al 1998;Psomas et al 1993;Nagata et al 1992;Nagata et al 1991), liquid chromatographytandem mass spectrometry (LC-MS-MS) (Chen et al 2005;Peng et al, 2005), gas chromatography (Pfenning et al 1998;2000;Plomp and Maes 1976;Gazzaning et al 1973) and gas chromatography -mass spectrometry (GC-MS) (Nagata and Okaj 1996;Nakagawa et al 1975).…”

Section: Introductionmentioning

confidence: 99%

Development and Optimization of an Indirect Enzyme‐Linked Immunosorbent Assay for Thiamphenicol

Hao¹,

Guo²,

Xu³

2006

Analytical Letters

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The specificity of an enzyme-linked immunosorbent assay (ELISA) was modified by changing the chemical structure of the haptenated coating antigen. A competitive indirect immunoassay was developed, evaluated, and validated to measure thiamphenicol (TAP) in eels. The working range of the assay was placed between 0.45 and 8.35 mg/L. Moreover, the influence of several physicochemical parameters, such as incubation time, ionic strength, detergent concentration, and pH, were studied. The specificity of this immunoassay was evaluated using 23 structurally related compounds. Finally, a good correlation was observed between the chromatographic and immunoassay techniques while analyzing five certified eel samples.

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