High-performance capillary electrophoretic determination of glutathione in human lymphocytes

Raggi, Maria Augusta; Mandrioli, Roberto; Sabbioni, C.; Mongiello, Francesca; Marini, Marina; Fanali, Salvatore

doi:10.1002/(sici)1520-667x(1998)10:6<503::aid-mcs5>3.0.co;2-k

Cited by 9 publications

(4 citation statements)

References 24 publications

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“…9B), which is quantified by its strong visible absorbance at 412 nm. However, utilizing a chromatographic methods, it becomes possible to separate the mixed disulfide produced in the reaction [110,[112][113][114].…”

Section: Compounds With Activated Double Bound Disulfides and The Otmentioning

confidence: 99%

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Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

Kuśmierek

Chwatko

Głowacki

et al. 2011

Journal of Chromatography B

111

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Section: Compounds With Activated Double Bound Disulfides and The Otmentioning

confidence: 99%

“…Reduced glutathione derivatized with Ellman's reagent and oxidized glutathione without derivatization were determined in water standard solution and human lymphocytes by Raggi et al [114].…”

Section: Capillary Electrophoresis Separationmentioning

confidence: 99%

Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

Kuśmierek

Chwatko

Głowacki

et al. 2011

Journal of Chromatography B

111

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“…In their complex, these data point to the clinical relevance of GSH and GSSG assessment. Several procedures for GSH and GSSG measurement have been reported, although, no reference method has been defined yet [9][10][11][12][13][14][15][16]. For the development of a sensitive and reliable method, the optimization of both pre-analytical and analytical procedures must be accomplished [9].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the principal parameters for the ultrarapid electrophoretic separation of reduced and oxidized glutathione by capillary electrophoresis

Carru

Zinellu

Sotgia

et al. 2003

Journal of Chromatography A

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“…High-performance capillary electrophoresis was used to analyze the levels of glutathione (GSH) and glutathione disulphide (GSSG) in plasma samples according to Raggi et al. 41 and Kong et al. 42 An Agilent 3D CE Instrument (Palo Alto, Ca) electrophoresis system with a DAD detector read the samples at 200 nm.…”

Section: Methodsmentioning

confidence: 99%

Effects of tocotrienol supplementation in Friedreich’s ataxia: A model of oxidative stress pathology

Bolotta

Pini

Abruzzo

et al. 2019

Exp Biol Med (Maywood)

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Friedreich’s ataxia is an autosomal recessive disorder characterized by impaired mitochondrial function, resulting in oxidative stress. In this study, we aimed at evaluating whether tocotrienol, a phytonutrient that diffuses easily in tissues with saturated fatty layers, could complement the current treatment with idebenone, a quinone analogue with antioxidant properties. Five young Friedreich’s ataxia patients received a low-dose tocotrienol supplementation (5 mg/kg/day), while not discontinuing idebenone treatment. Several oxidative stress markers and biological parameters related to oxidative stress were evaluated at the time of initiation of treatment and 2 and 12 months post-treatment. Some oxidative stress-related parameters and some inflammation indices were altered in Friedreich’s ataxia patients taking idebenone alone and tended to be normal values following tocotrienol supplementation; likewise, a cardiac magnetic resonance study showed some improvement following one-year tocotrienol treatment. The pathway by which tocotrienol affects the Nrf2 modulation of hepcidin gene expression, a peptide involved in iron handling and in inflammatory responses, is viewed in the light of the disruption of the iron intracellular distribution and of the Nrf2 anergy characterizing Friedreich’s ataxia. This research provides a suitable model to analyze the efficacy of therapeutic strategies able to counteract the excess free radicals in Friedreich’s ataxia, and paves the way to long-term clinical studies. Impact statement Oxidative stress is involved in the pathogenesis of Friedreich's ataxia (FRDA), a genetic disorder causing neurodegeneration due to the dramatic reduction in the expression of frataxin. To date, no cure is available for FRDA patients. In some countries, FRDA patients assume idebenone in order to counteract the effects of frataxin deficiency. We demonstrate that idebenone treatment alone is not able to abrogate oxidative stress in FRDA patients, whereas the combined treatment with tocotrienols might be more efficient and perhaps produce clinical improvement. In fact, a decrease in oxidative stress and inflammation markers can be seen after two months and is more pronounced after one year of treatment. This is, in our opinion, valuable information for clinicians, since idebenone is the treatment of choice for FRDA patients in some countries.

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High-performance capillary electrophoretic determination of glutathione in human lymphocytes

Cited by 9 publications

References 24 publications

Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

Optimization of the principal parameters for the ultrarapid electrophoretic separation of reduced and oxidized glutathione by capillary electrophoresis

Effects of tocotrienol supplementation in Friedreich’s ataxia: A model of oxidative stress pathology

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