High Performance Capillary Electrophoresis Characteristic Fingerprint of Schisandra chinensis

Abstract

Background: The quality control indexes detected by the existing methods are less and lack specificity, and cannot truly reflect their intrinsic quality because of the similarity of Schisandra chinensis and Schisandra sphenanthera in both their appearance and their main chemical composition. Therefore, it is urgent to establish an effective method for the quality control of the both. High-performance capillary electrophoresis (HPCE) method was used to establish the fingerprints of Schisandra medicinal materials. Methods: The HPCE was performed, in which a 50µm × 50cm (the effective length was 41.5cm) uncoated fused silica capillary column was applied, the buffer solution was 10 mM phosphate buffer (pH 8)-37.5 mM SDS-35% acetonitrile (v/v), the working voltage was 28 kV, the temperature was 25°C, the samples were injected at 50mbar pressure for 3s, and the detection wavelength was 214nm. The chromatographic fingerprints of samples were analyzed using “traditional Chinese medicine chromatographic fingerprint similarity evaluation system 2004A version” software and cluster analysis. Results: 10 common peaks were confirmed and 8 components were identified in the HPCE fingerprints of 20 batches of Schisandra chinensis and Schisandra sphenanthera, and the results of clustering were consistent with those of similarity evaluation. Conclusion: This method should be accurate and reliable, and can provide a scientific basis for the quality evaluation of Schisandra medicinal materials.

Development of Electrochemical Oscillation Method for Identification of Prunus persica, Prunus davidiana, and Prunus armeniaca Nuts

“…At present, the common fingerprints of traditional herbal medicine are thin layer chromatography fingerprint (Szeremeta et al, 2017 ; Sibug-Torres et al, 2019a , b ), high-performance liquid chromatography fingerprint (Li et al, 2016 ; Xue et al, 2017 ; Esteki et al, 2019 ), gas chromatography fingerprint (Aliakbarzadeh et al, 2016 ; Shekari et al, 2018 ; Huang et al, 2019 ), high-performance capillary electrophoresis fingerprint (Hou et al, 2019 ; Sun et al, 2019 ), high-speed countercurrent chromatography fingerprint (Gan et al, 2016 ; Wang et al, 2020 ), ultraviolet fingerprint, infrared fingerprint (Custers et al, 2017 ; Dai et al, 2019 ), nuclear magnetic resonance fingerprint (Sun et al, 2018 ; Flores et al, 2020 ), mass spectrometry fingerprint (Yang and Deng, 2016 ; Kharbach et al, 2020 ), X-ray diffraction fingerprint (Chen et al, 2017 ; Devi et al, 2017 ), immunoassay fingerprint (Cui et al, 2019 ), and DNA fingerprint (Pawar et al, 2017 ; Selvakumari et al, 2017 ). These methods can be used for in-depth research on traditional herbal medicine, but proper pretreatment of traditional herbal medicine is required.…”

Effects of Schisandra chinensis Extract on the Learning and Memory Ability of Mice With Learning and Memory Disorders

“…[14][15][16] Some studies have shown that S. chinensis can improve the learning and memory disorders of mice, but these studies lacked a systematic approach, and its mechanism was not studied in sufficient depth. 17 In this study, the models of memory acquisition disorder, memory consolidation disorder, memory reproduction disorder, and aging in mice were established by intraperitoneal injection of scopolamine, chloramphenicol, 40% ethanol, and subcutaneous injection of d-galactose, respectively; the improvement of Schisandra chinensis extract (SCE) on the learning and memory disorders induced by the different agents was observed systematically, and the mechanism explored, which was expected to provide a basis for S. chinensis being used as either a medicine or health food.…”