High performance and stability of a hemoglobin-biosensor based on an ionic liquid as nonaqueous media for hydrogen peroxide monitoring

Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4/Chitosan Core-Shell Microspheres

“…K m app is calculated to be 0.29 mM, which is smaller than that those of 0.68 mM for Hb in hydrophilic matrix film modified electrode [41], 0.75 mM for Hb in chitosan/nano-CaCO 3 composite electrode [43], and 0.898 mM for Hb in silica sol-gel film modified electrode [44]. These results suggest that the immobilized Hb remains its activity to H 2 O 2 reduction and the biosensor has high affinity to H 2 O 2 .…”

“…Figure 8 shows the chronoamperometric curve of the biosensor for successive addition of H 2 O 2 in a magnetic stirring condition. When the H 2 O 2 was added into the pH 8.0 PBS containing 6.0 × 10 −4 M HQ, the biosensor responded rapidly to the substrate increase and achieved 95% of the steady current in 10 s. It is faster than the reported results of HRP that was immobilized on a hydrophilic matrix film modified glassy carbon electrode (40 s) [ 41 ]. This may result from the Fe 3 O 4 nanoparticles that can facilitate the transfer of electrons.…”

Use of ionic liquids in sol-gel; ionogels and applications

“…Some examples are based on the immobilization of enzyme-IL systems in organic hydrogels [111]. New hydrid organic-inorganic siloxane-based hydrogels entrapping IL have been recently developed for similar uses [112].…”

Highly sensitive amperometric biosensors for phenols based on polyaniline–ionic liquid–carbon nanofiber composite

“…More recently, ionic liquids have gained increasing interest in the area of electrochemistry for their wide potential window and extremely high ionic conductivity as supporting electrolyte (Xiong et al, 2007). Furthermore, they have been incorporated to conventional materials, such as chitosan (Lu et al, 2006), carbon nanotubes , polymer (Mu, 2007;Kim et al, 2008), and Nafion for improving conductivity and promoting the electron transfer Xi et al, 2008).…”