High-performance affinity chromatography system for the rapid, efficient assay of glycated albumin

Basic Performance of an Enzymatic Method for Glycated Albumin and Reference Range Determination

“…Affinity chromatography, 15,16 ion exchange chromatography, 7,17 and HPLC methods 21,22 define GA level as the ratio of GA peak area to total area of albumin peak, and the target molecule of GA measurement is the glycated albumin molecule. On the other hand, TBA 7 and enzymatic assays 23,24 define the GA level as the ratio of total glycated amino acid concentration to albumin concentration.…”

“…Historically, GA has been measured by several methods including boronate affinity chromatography, 15,16 ion exchange chromatography, 7,17 thiobarbituric acid (TBA) assay, 7 immunoassay, 18,19 enzymelinked boronate-immunoassay, 20 and high-performance liquid chromatography (HPLC). 21,22 Table 1 summarizes the normal ranges and analytes measured by these GA measurement methods.…”

“…Automated HPLC assay of GA was performed as described in other studies. 21,22 Molecular weights of purified GA and nonglycated albumin (NGA) were measured by a mass spectrometry system. Measurements were performed using QSTAR ® Pulsar i mass spectrometer (Applied Biosystems, Carlsbad, CA).…”

Point of care testing system via enzymatic method for the rapid, efficient assay of glycated albumin

“…However, the dry chemistry system tends to exhibit higher values compared with the liquid chemistry system, as shown by a slope of 1.38. In the newly proposed method for determining the GA value, based on the calibration curve, the difference between the conventional methods was calibrated (Shima et al, 1988;Yasukawa et al, 1992). The existence of linearity between the conventional and new method is important, but differences between the slopes are not, as this could be calibrated using the variable.…”

Chromatographic Techniques for the Characterization of Proteins

“…(1989) present an overview of commercially available columns including a variety of ligands, such as boronic acid for the analysis of carbohydrates, cephalosporin for the analysis of J3-lactamase, and glucosamine for the analysis of concanavalin A. An application of boronate affinity chromatography can be found in Yasukawa et al (1992), who describe a rapid assay of glycosylated albumin.…”