High Paip1 Expression as a Potential Prognostic Marker in Hepatocellular Carcinoma

Abstract

Background/Aim: Translation plays an important role in the carcinogenesis of various human tumors. Paip1 and eIF4A1 are translation-associated proteins that mediate the function of eukaryotic initiation factor 4F complex. This study aimed to analyse the relationship between the expression status of Paip1 and eIF4A1 and clinicopathologic features in hepatocellular carcinoma (HCC). Materials and Methods: Immunohistochemical analysis was used to evaluate the expression status of Paip1 and eIF4A1. Two pathologists independently interpreted the immunostained slides. The prognostic value of Paip1 and eIF4A1 was evaluated by the Kaplan-Meier plotter. Results: Among 173 HCC patients, 28 (16.1%) and 46 (26.6%) belonged in the Paip1 and eIF4A1 high-expression groups. High expression of Paip1 and eIF4A1 was associated with advanced TNM stage and more frequent vascular tumor invasion. Univariate analysis indicated that high Paip1 expression was associated with worse five-year overall survival (OS). Public dataset analysis by Kaplan-Meier plotter revealed that high mRNA expression of Paip1, and not of eIF4A1, was significantly associated with worse five-year OS and disease-free survival. Conclusion: Paip1 expression has a potential prognostic value in human HCC.Translation is one of the core processes in posttranscriptional gene expression regulation and is an important factor in tumorigenesis (1, 2). The eIF4F complex is involved in eukaryote translation initiation and consists of eIF4E, eIF4G, and eIF4A. Poly (A)-binding protein (PABP) binds to eIF4G to induce mRNA circularization and stimulate mRNA translation. Poly (A)-binding proteininteracting protein 1 (Paip1) is encoded by the PAIP1 gene and binds to PABP to regulate its activity (3). High expression of Paip1 has been reported to be associated with poor overall survival in various tumors, including gastric, breast, pancreatic, uterine, and cervical cancers (4-7).eIF4A1, which is one of three isoforms of eIF4A, is a constituent of the eIF4F complex and an ATP-dependent RNA helicase that plays a critical role in translation initiation by unwinding 5'-UTR structures of mRNA. Several reports suggest that eIF4A1 might be involved in the process of carcinogenesis (8-10).This study aimed to examine the levels of Paip1 and eIF4A1 proteins that regulate human translation initiation in hepatocellular carcinoma (HCC). We investigated their association with various clinicopathological parameters. In addition, we divided patients into low-and high-expression groups and analyzed whether there was a significant difference in survival.

Effect of PAIP1 on the metastatic potential and prognostic significance in oral squamous cell carcinoma

“…Indeed, we found a similar trend in the PAIP1 mRNA and protein level overexpression in oral cancers when compared to healthy controls (Fig. 1 ) 11 – 13 , 15 , 19 . Higher PAIP1 expression was a frequent event in cohorts of patients with OSCC, associated with higher risk and positive correlation with greater tumor size, LNM, and advanced stages of the tumor and reduced survival (Fig.…”

“…Higher PAIP1 expression was a frequent event in cohorts of patients with OSCC, associated with higher risk and positive correlation with greater tumor size, LNM, and advanced stages of the tumor and reduced survival (Fig. 2 and Fig S3 ), consistent with that of various tumors 11 – 13 , 15 , 19 . Xie et al described similar findings in tongue carcinomas, but the representative data does not corroborate with the conclusion 20 .…”

Circ_0005576 Exerts an Oncogenic Role in Cervical Cancer via miR-1305-Dependent Regulation of PAIP1

“…Numerous studies have shown that PAIP1 was upregulated and was associated with the development of multifarious cancers. For example, Kim et al found that PAIP1 expression was elevated in hepatocellular carcinoma and high expression of PAIP1 was associated with tumor invasion and worse five-year overall survival (OS) [ 32 ]. Guan et al presented that PAIP1 was overexpressed in pancreatic cancer tissues, and depletion of PAIP1 signally lessened cell proliferation, metastasis and angiogenesis [ 33 ].…”

PAIP1 regulates expression of immune and inflammatory response associated genes at transcript level in liver cancer cell

“…Results of these studies indicated that PAIP1 is a potential prognostic biomarker for cancer. It is notable that PAIP1 is highly expressed in HCC tissues compared with the normal liver tissue, and the expression of PAIP1 in HCC tissues is significantly correlated with the prognosis of patients ( Kim et al, 2020 ). However, the roles and underlying molecular mechanism of PAIP1 in liver cancer are still unclear.…”