High oxygen facilitates wound induction of suberin polyphenolics in kiwifruit

Wei, Xiaopeng; Mao, Linchun; Han, Xueyuan; Lu, Wenjing; Xie, Dandan; Ren, Xingchen; Zhao, Yuying

doi:10.1002/jsfa.8709

Cited by 27 publications

(15 citation statements)

References 45 publications

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“…Suberization is a common plant response to wounding and plays a critical role in formation of an impervious layer (Han et al, 2017;Wei et al, 2018). The aliphatics are considered as critical composition in controlling excess water loss and pathogen infection from wound tissues (Lulai and Corsini, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…Xuxiang), free from wound injury and infection, were harvested at commercial maturity in Hangzhou, Zhejiang Provence, China. Surface sterilization and wound treatment of fruit were performed according to our previous study (Wei et al, 2018). Subsequently, the kiwifruit halves were treated with sterile water, FLD and ABA via vacuum infiltration as described previously (Tao et al, 2016), and were placed into a sterile incubator (HWS, Ningbo Southeast Instrument Co., China) for wound healing at 20°C and 85% RH (relative humidity).…”

Section: Plants Materials and Treatmentsmentioning

confidence: 99%

“…Wound-induced suberization and development of an impervious layer at wound sites are the major wound healing processes of plants, which can be regulated by a number of endogenous and exogenous factors (Boher et al, 2013;Fugate et al, 2016;Wei et al, 2018). Major plant hormones, such as abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA) and cytokinin, have been reported to regulate suberization processes (Mitchell and Van Staden, 1983;Lulai et al, 2008;Lulai et al, 2011;Boher et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Three Transcription Activators of ABA Signaling Positively Regulate Suberin Monomer Synthesis by Activating Cytochrome P450 CYP86A1 in Kiwifruit

Wei

Mao

et al. 2020

Front. Plant Sci.

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Wound attack stimulates accumulation of abscisic acid (ABA) that activates a number of genes associated with wound suberization of plants. Cytochrome P450 fatty acid whydroxylase CYP86A1 catalyzes w-hydroxylation of fatty acids to form the wfunctionalized monomers that play a pivotal role in suberin synthesis. However, the transcriptional regulation of ABA signaling on AchnCYP86A1 has not been characterized in kiwifruit. In this study, AchnCYP86A1, a kiwifruit homolog of Arabidopsis AtCYP86A1, was isolated. AchnCYP86A1-overexpressed N. benthamiana leaves displayed that the AchnCYP86A1 functioned as a fatty acid w-hydroxylase associated with synthesis of suberin monomer. The regulatory function of three transcription factors (TFs, including AchnMYC2, AchnMYB41 and AchnMYB107) on AchnCYP86A1 was identified. All the three TFs were localized in nucleus and could individually interact with AchnCYP86A1 promoter to activate gene expression in yeast one-hybrid and dual-luciferase assays. The findings were further demonstrated in transient overexpressed N. benthamiana, in which all TFs notably elevated the expression of aliphatic synthesis genes including CYP86A1 and the accumulation of w-hydroxyacids, a, w-diacids, fatty acids and primary alcohols. Moreover, exogenous ABA induced the expression of AchnMYC2, AchnMYB41 and AchnMYB107 that promoted AchnCYP86A1 involving in suberin monomer formation. Contrary to the inductive effects of ABA, however, fluridone (an inhibitor of ABA biosynthesis) inhibited the three TFs expression and suberin monomer formation. These results indicate that AchnMYC2, AchnMYB41 and AchnMYB107 positively regulate suberin monomer synthesis by activating AchnCYP86A1 promoter in response to ABA.

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Section: Discussionmentioning

confidence: 99%

Section: Plants Materials and Treatmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Three Transcription Activators of ABA Signaling Positively Regulate Suberin Monomer Synthesis by Activating Cytochrome P450 CYP86A1 in Kiwifruit

Wei

Mao

et al. 2020

Front. Plant Sci.

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“…These wounds could be healed by suberin-containing structural barriers, such as periderm, and protect the plant from pathogenic infections (Soler et al, 2011). Because wound-induced suberization accelerates in the presence of higher oxygen (Wei et al, 2018), FOC penetration is more difficult in storage than in soil if the suberization rate of the external basal plate tissue outcompetes the rate of pathogenic development. A histological study of basal plate penetration after artificial inoculation of mature bulbs during storage could indicate the reason for the mechanical resistance of FOC.…”

Section: Discussionmentioning

confidence: 99%

An Artificial Inoculation Method to Select Mature Onion Bulbs Resistant to Fusarium Basal Rot

Mandal¹,

Cramer²

2020

horts

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Fusarium basal rot (FBR) of onion, which is caused by Fusarium oxysporum f.sp. cepae (Hanzawa) Snyder & Hansen (FOC) results in a substantial loss of marketable bulbs worldwide. One of the main reasons for the lack of FBR-resistant short-day cultivars is the unreliable screening methods available for the mature bulb stage when significant economic damage occurs. The objective of this study was to develop an artificial inoculation method with better quantification of inoculum for an effective selection of FBR-resistant mature onion bulbs. Mature bulbs of seven New Mexican short-day onion cultivars, along with susceptible and tolerant controls, were selected and evaluated for FBR resistance using mycelial and conidial inoculation methods, respectively. Transversely cut basal plates of mature bulbs were inoculated artificially with mycelia or conidia (12 × 105 spores/mL in 2014 and 3 × 105 spores/mL in 2015 embedded in potato dextrose agar plug) of a virulent FOC isolate ‘CSC-515’. Mature bulb evaluation using a visual rating scale (1 = no disease; 9 = >70% basal plate infected) revealed a high degree of FBR severity and incidence irrespective of the genetic background of the cultivars, minimizing the chance of disease escape, which is a significant problem in field inoculation. An attempt to inoculate intact basal plates postharvest resulted in minimal disease development, suggesting that mechanical resistance was conferred by the dry outer layer of the basal plate. The high selection pressure conferred by the conidial inoculation method developed in this study can effectively screen FBR-resistant onion bulbs to replace an unreliable field screening. Concentrations of the conidia lower than 3 × 105 spores/mL are recommended to detect subtle genetic differences in FBR resistance among the onion cultivars and their selected population.

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“…Enzyme extracts for phenylalanine ammonialyase (PAL) and peroxidase (POD) were prepared according to Tao et al () and Wei et al (). Around 0.1 g of roots were frozen in liquid nitrogen, milled and homogenized in 1 ml mixture of 100‐mM Na 2 B 4 O 7 buffer (pH 8.8) containing 1‐mM EDTA and 3% polyvinylpyrrolidone (PVP) for PAL assay, and 1‐ml mixture of 50‐mM Na 3 PO 4 buffer (pH 7.8) containing 1‐mM EDTA and 2% PVP for POD assay.…”

Section: Methodsmentioning

confidence: 99%

Abscisic acid‐mediated modifications of radial apoplastic transport pathway play a key role in cadmium uptake in hyperaccumulator Sedum alfredii

Jupa

Liu

et al. 2019

Plant Cell & Environment

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Abscisic acid (ABA) is a key phytohormone underlying plant resistance to toxic metals.However, regulatory effects of ABA on apoplastic transport in roots and consequences for uptake of metal ions are poorly understood. Here, we demonstrate how ABA regulates development of apoplastic barriers in roots of two ecotypes of Sedum alfredii and assess effects on cadmium (Cd) uptake. Under Cd treatment, increased endogenous ABA level was detected in roots of nonhyperaccumulating ecotype (NHE) due to up-regulated expressions of ABA biosynthesis genes (SaABA2, SaNCED), but no change was observed in hyperaccumulating ecotype (HE). Simultaneously, endodermal Casparian strips (CSs) and suberin lamellae (SL) were deposited closer to root tips of NHE compared with HE. Interestingly, the vessel-to-CSs overlap was identified as an ABA-driven anatomical trait. Results of correlation analyses and exogenous applications of ABA/Abamine indicate that ABA regulates development of both types of apoplastic barriers through promoting activities of phenylalanine ammonialyase, peroxidase, and expressions of suberin-related genes (SaCYP86A1, SaGPAT5, and SaKCS20). Using scanning ion-selected electrode technique and PTS tracer confirmed that ABA-promoted deposition of CSs and SL significantly reduced Cd entrance into root stele. Therefore, maintenance of low ABA levels in HE minimized deposition of apoplastic barriers and allowed maximization of Cd uptake via apoplastic pathway.

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High oxygen facilitates wound induction of suberin polyphenolics in kiwifruit

Abstract: Oxygen is required for wound-induced melanin and SPPs formation, and high oxygen is effective in promoting wound suberization in postharvest kiwifruit. © 2017 Society of Chemical Industry.

Cited by 27 publications

References 45 publications

Three Transcription Activators of ABA Signaling Positively Regulate Suberin Monomer Synthesis by Activating Cytochrome P450 CYP86A1 in Kiwifruit

Three Transcription Activators of ABA Signaling Positively Regulate Suberin Monomer Synthesis by Activating Cytochrome P450 CYP86A1 in Kiwifruit

An Artificial Inoculation Method to Select Mature Onion Bulbs Resistant to Fusarium Basal Rot

Abscisic acid‐mediated modifications of radial apoplastic transport pathway play a key role in cadmium uptake in hyperaccumulator Sedum alfredii

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