High oxygen concentrations inhibit Acanthamoeba spp.

Sifaoui, Inés; Yanes, Eulalia Capote; Reyes-Batllé, María; Rodríguez-Expósito, Rubén L.; Bazzocchi, Isabel L.; Jiménez, Ignacio A.; Piñero, José E.; Weaver, Lindell K.

doi:10.1007/s00436-021-07219-3

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…The last one (OTU 29) is affiliated to the candidatus Odyssella genus, whose species type was described as an obligate intracellular parasite of Acanthamoeba sp. 63 a group of amoeba forming cysts under anoxic conditions, capable of revival under oxygenated conditions 64 and sensitive to high oxygen concentration. 65…”

Section: Resultsmentioning

confidence: 99%

Influence of microorganisms on uranium release from mining-impacted lake sediments under various oxygenation conditions

Seder-Colomina

Mangeret

Bauda

et al. 2022

Environ. Sci.: Processes Impacts

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Section: Resultsmentioning

confidence: 99%

Influence of microorganisms on uranium release from mining-impacted lake sediments under various oxygenation conditions

Seder-Colomina

Mangeret

Bauda

et al. 2022

Environ. Sci.: Processes Impacts

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Section: Mechanisms Of Stress In Pathogenic Amoebae During Accumulati...mentioning

confidence: 99%

“…The literature reports several molecules able to regulate the Acanthamoeba OS response. Exposition to pure oxygen has been shown to increase production in ROS [37], whereas chlorhexidine increases the ROS levels in Acanthamoeba through a reduction in the activity of antioxidant enzymes such as the superoxide dismutase (SOD) and the flavin adenine dinucleotide-fumarate reductase (NADH-FRD) [38]. Conversely, statins were shown to provoke a collapse in the mitochondrial potential and ATP levels, cytoskeleton disassembly, and ROS generation [39].…”

Section: Mechanisms Of Stress In Pathogenic Amoebae During Accumulati...mentioning

confidence: 99%

Encystation and Stress Responses under the Control of Ubiquitin-like Proteins in Pathogenic Amoebae

Samba-Louaka,

Labruyère,

Matondo

et al. 2023

Microorganisms

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Amoebae found in aquatic and terrestrial environments encompass various pathogenic species, including the parasite Entamoeba histolytica and the free-living Acanthamoeba castellanii. Both microorganisms pose significant threats to public health, capable of inducing life-threatening effects on humans. These amoebae exist in two cellular forms: trophozoites and cysts. The trophozoite stage is the form used for growth and reproduction while the cyst stage is the resistant and disseminating form. Cysts occur after cellular metabolism slowdown due to nutritional deprivation or the appearance of environmental conditions unfavourable to the amoebae’s growth and division. The initiation of encystation is accompanied by the activation of stress responses, and scarce data indicate that encystation shares factors and mechanisms identified in stress responses occurring in trophozoites exposed to toxic compounds derived from human immune defence. Although some “omics” analyses have explored how amoebae respond to diverse stresses, these studies remain limited and rarely report post-translational modifications that would provide knowledge on the molecular mechanisms underlying amoebae-specific stress responses. In this review, we discuss ubiquitin-like proteins associated with encystation and cell survival during oxidative damage. We aim to shed light on the signalling pathways involved in amoebic defence mechanisms, with a focus on their potential clinical implications against pathogenic amoebae, addressing the pressing need for effective therapies.

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“…Antimicrobial topical treatments are available for AK infection and usually comprise a combination of propamidine isethionate and neomycin or chlorhexidine, while contact lens disinfectants often include chlorhexidine [ 2 ]. Notably, a plethora of studies have shown that many of the contact lens disinfectants commercially available in the market are ineffective against Acanthamoeba [ 9 , 10 ], suggesting the need to identify the compound(s) that are biologically relevant, cost effective, effective against cysts and trophozoites, and nontoxic, and which can be used as disinfectants and/or therapeutic agents [ 11 , 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Plant-Based Metabolites as Disinfectants against Acanthamoeba castellanii

et al. 2022

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Due to global warming, coupled with global water shortages and the reliance of the public on household water tanks, especially in developing countries, it is anticipated that infections caused by free-living amoebae such as Acanthamoeba will rise. Thus, the development of novel disinfectant(s) which can target pathogenic free-living amoebae effectively is warranted. Herein, we extracted and isolated several plant-based secondary metabolites as novel disinfectants for use against pathogenic Acanthamoeba. The identity of the compounds was confirmed by nuclear magnetic resonance and tested for antiamoebic activities against clinical isolate of A. castellanii, belonging to the T4 genotype. Amoebicidal assays revealed that the compounds tested showed antiamoebic properties. Betulinic acid and betulin exhibited parasite killing of more than 65%. When tested against the cyst stage, betulinic acid, betulin, and vanillic acid inhibited both encystation and excystation processes. Furthermore, the plant-based metabolites significantly inhibited the binding capability of A. castellanii to host cells. Finally, most of the tested compounds displayed minimal cytotoxic activities against human cells and noticeably perturbed amoeba-mediated host cell cytotoxicity. Notably, both alkaloid and betulinic acid showed 20% cytotoxic effects, whereas betulin and lupeol had cytotoxic effects of 24% and 30%, respectively. Overall, our findings indicate that plant-based natural compounds demonstrate anti-Acanthamoebic properties, and they have potential candidates for water disinfectants or contact lens disinfecting solutions, as well as possible therapeutic drugs against Acanthamoeba infections.

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High oxygen concentrations inhibit Acanthamoeba spp.

Cited by 5 publications

References 27 publications

Influence of microorganisms on uranium release from mining-impacted lake sediments under various oxygenation conditions

Influence of microorganisms on uranium release from mining-impacted lake sediments under various oxygenation conditions

Encystation and Stress Responses under the Control of Ubiquitin-like Proteins in Pathogenic Amoebae

Novel Plant-Based Metabolites as Disinfectants against Acanthamoeba castellanii

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