High oxygen atmosphere improves human follicle development in organ cultures of ovarian cortical tissues in vitro

Morimoto, Yoshiharu; Oku, Yusuke; Sonoda, M.; Haruki, A.; Ito, Katsuhiko; Hashimoto, S.; Fukuda, Aisaku

doi:10.1093/humrep/dem314

Cited by 24 publications

(21 citation statements)

References 34 publications

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“…In line with this, some approaches have been taken to maintain gametes and embryos in a safe atmosphere from the very beginning of production. However, many IVF laboratories have attempted other intermediate options, such as setting incubators to low O 2 tension to render certain benefits for embryo viability and implantation potential at least during the time that human embryos remain inside incubators during in vitro culture (8)(9)(10)(11)(12)(13)(14)(15).…”

mentioning

confidence: 99%

Reduced oxygen tension improves embryo quality but not clinical pregnancy rates: a randomized clinical study into ovum donation cycles

Santos

Gámiz

Albert

et al. 2013

Fertility and Sterility

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mentioning

confidence: 99%

Reduced oxygen tension improves embryo quality but not clinical pregnancy rates: a randomized clinical study into ovum donation cycles

Santos

Gámiz

Albert

et al. 2013

Fertility and Sterility

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“…Furthermore, some types of cells proliferated better in the 20% oxygen environment than in a reduced oxygen environment [24]. When a large amount of tissue or an organ is cultured, the ambient oxygen concentration is usually used [25][26][27][28], and in some studies, even higher oxygen concentrations have been applied [29][30][31]. It therefore seems necessary to find an optimal oxygen concentration for each type of target cell and/or tissue according to its size.…”

mentioning

confidence: 99%

Optimization of Oxygen Concentration for Growing Bovine Oocytes <i>In Vitro</i>: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes

Hirao

Shimizu

Iga

et al. 2012

Journal of Reproduction and Development

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Abstract:The oxygen environment in cell culture has a significant impact on the health and performance of cells. Here, we compared the effects of reduced (5%) and ambient (20%) oxygen concentrations on bovine oocyte-granulosa cell complexes, each containing a growing oocyte 90-102 µm in diameter, cultured for 14 days. Both oxygen concentrations showed some advantages and disadvantages; in 5% oxygen, the survival rate of oocytes was significantly higher than in 20% oxygen, but the resulting oocytes were significantly smaller, which was a serious disadvantage. During the first 4 days of culture, the growth and viability of oocytes were satisfactory using 5% oxygen. This observation led us to examine the effect of changing the oxygen concentration from 5% to 20% on Day 4 in order to minimize the expected disadvantages of constant 5% and 20% oxygen. The largest population of fully grown oocytes was obtained from cultures in which the oxygen concentration was changed in this way, which also led to higher oocyte viability than in constant 20% oxygen. A similar tendency was found in the frequency of oocytes becoming blastocysts after in vitro fertilization. Surviving oocytes eventually became located within an enlarged dome-like structure, and although the 5% oxygen environment may have been appropriate for oocyte growth in the early stages, 20% oxygen may have been necessary for the growth of oocytes in the dome-like structure. These results indicate an effective way of modulating oxygen concentration according to the growth of oocyte-granulosa cell complexes in vitro. Key words: Bovine, Granulosa cell, Growing oocyte, In vitro, Oxygen concentration (J. Reprod. Dev. 58: [204][205][206][207][208][209][210][211] 2012) M ost of the growing oocytes stored in the mammalian ovary are eventually lost, and in order to be able to utilize them, it is important to develop culture systems that effectively support oocyte growth and the acquisition of developmental competence. We have previously described a 2-week culture system [1] in which bovine oocytes obtained from early antral follicles are able to complete the growth phase. However, the resulting oocytes are on average smaller than those grown in follicles in vivo. Further improvement of the culture system is therefore necessary.Our previous work comparing bovine oocyte growth in reduced (5%) and ambient (20%) oxygen concentrations [2] suggests the importance of oxygen optimization in determining culture conditions. The oxygen environment has significant effects on cell activities in culture in various cell types, such as somatic cells [3,4], embryos [5,6] and stem cells [7]. Although a universal optimal oxygen concentration for cell culture has not been determined, many studies on embryos [8][9][10], preantral follicles [11], testicular germ cells [12] and other cells [13][14][15][16][17] have suggested that a reduced oxygen concentration gives better results than an ambient oxygen concentration. A partial explanation for this would be that there is less cell damage asso...

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“…According to Morimoto et al (21), ovarian tissue culture under higher oxygen concentration, which increases oxygen penetration of tissue, may improve follicle development (21). At the same time, under the higher oxygen conditions, the simultaneous use of antioxidants to reduce the oxidative stress caused by reactive oxygen species may be required to promote follicle survival and growth.…”

Section: Resultsmentioning

confidence: 99%

“…In 2007, Morimoto et al (21) investigated the effect of oxygen concentration in organ culture. Ovarian tissue was obtained from women 26 to 35 years old during operations to remove benign tumors (n ¼ 12) or during caesarean section (n ¼ 16).…”

Section: Known Mechanisms Driving Early and Late Follicle Developmentmentioning

confidence: 99%

Culture of cryopreserved ovarian tissue: state of the art in 2008

Fabbri

Pasquinelli

Keane

et al. 2009

Fertility and Sterility

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High oxygen atmosphere improves human follicle development in organ cultures of ovarian cortical tissues in vitro

Abstract: Human ovarian tissues can be cultured for 15 days under high oxygen concentration with the organ culture system used here. This technique could make it possible to utilize ovarian tissue for preservation of reproductive competence in cancer patients.

Cited by 24 publications

References 34 publications

Reduced oxygen tension improves embryo quality but not clinical pregnancy rates: a randomized clinical study into ovum donation cycles

Reduced oxygen tension improves embryo quality but not clinical pregnancy rates: a randomized clinical study into ovum donation cycles

Optimization of Oxygen Concentration for Growing Bovine Oocytes <i>In Vitro</i>: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes

Culture of cryopreserved ovarian tissue: state of the art in 2008

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