High-order Michaelis-Menten equations allow inference of hidden kinetic parameters in enzyme catalysis
Divya Singh,
Tal Robin,
Michael Urbakh
et al.
Abstract:Single-molecule measurements provide a platform for investigating the dynamical properties of enzymatic reactions. To this end, the single-molecule Michaelis-Menten equation was instrumental as it asserts that the first moment of the enzymatic turnover time depends linearly on the reciprocal of the substrate concentration. This, in turn, provides robust and convenient means to determine the maximal turnover rate and the Michaelis-Menten constant. Yet, the information provided by these parameters is incomplete … Show more
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