High Occurrence of Multiresistant<i>Salmonella</i>Infantis in Retail Meat in Ecuador

Mejía, Lorena; Vela, Gabriela; Zapata, Sonia

doi:10.1089/fpd.2020.2808

“…S. enterica Infantis has been the most prevalent serovar isolated from fresh poultry meat and broiler flocks from across Europe [6,54] and human S. enterica Infantis infections are often associated with poultry meat [2,6,[55][56][57][58]. Between 2014 and 2018 in England and Wales, human salmonellosis associated with S. enterica Infantis has been increasing, and this serovar has now become the fifth most widespread after S. enterica Enteritidis (n=12 548, 290.6 %), S. enterica Typhimurium including monophasic (n=7930, 180.7 %), S. enterica Newport (n=1372, 30.2 %) and S. enterica Typhi (n=1120, 20.6 %) (PHE data).…”

Section: Discussionmentioning

confidence: 99%

“… enterica Infantis has been the most prevalent serovar isolated from fresh poultry meat and broiler flocks from across Europe [6, 54] and human S . enterica Infantis infections are often associated with poultry meat [2, 6, 55–58]. Between 2014 and 2018 in England and Wales, human salmonellosis associated with S .…”

Section: Discussionmentioning

confidence: 99%

Characterization of a pESI-like plasmid and analysis of multidrug-resistant Salmonella enterica Infantis isolates in England and Wales

Lee

¹

,

Mattock

²

,

Greig

³

et al. 2021

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Salmonella enterica serovar Infantis is the fifth most common Salmonella serovar isolated in England and Wales. Epidemiological, genotyping and antimicrobial-resistance data for S . enterica Infantis isolates were used to analyse English and Welsh demographics over a 5 year period. Travel cases associated with S . enterica Infantis were mainly from Asia, followed by cases from Europe and North America. Since 2000, increasing numbers of S . enterica Infantis had multidrug resistance determinants harboured on a large plasmid termed ‘plasmid of emerging S . enterica Infantis’ (pESI). Between 2013 and 2018, 42 S . enterica Infantis isolates were isolated from humans and food that harboured resistance determinants to multiple antimicrobial classes present on a pESI-like plasmid, including extended-spectrum β-lactamases (ESBLs; bla CTX-M-65). Nanopore sequencing of an ESBL-producing human S . enterica Infantis isolate indicated the presence of two regions on an IncFIB pESI-like plasmid harbouring multiple resistance genes. Phylogenetic analysis of the English and Welsh S . enterica Infantis population indicated that the majority of multidrug-resistant isolates harbouring the pESI-like plasmid belonged to a single clade maintained within the population. The bla CTX-M-65 ESBL isolates first isolated in 2013 comprise a lineage within this clade, which was mainly associated with South America. Our data, therefore, show the emergence of a stable resistant clone that has been in circulation for some time in the human population in England and Wales, highlighting the necessity of monitoring resistance in this serovar.

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“…Antibacterial activity has been studied mainly for both Gram‐positive and Gram‐negative pathogens associated with the wound, skin, and mucosal infections (Abdel‐Shafi et al., 2022; Diekema et al., 2019; Mejia et al., 2021; Montero et al., 2021), such as Staphylococcus aureus , Pseudomonas aeruginosa , Escherichia coli , and Salmonella enterica subsp. enterica serovar Typhimurium.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial activity of stingless bee honey (Tribe: Meliponini) on clinical and foodborne pathogens: A systematic review and meta‐analysis

Cabezas‐Mera,

Cedeño‐Pinargote,

Tejera

et al. 2024

Food Frontiers

0

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Honey produced by stingless bees (Tribe: Meliponini) is well‐known for their medicinal and antimicrobial properties from their diverse content of bioactive compounds. However, it has not been possible to fully characterize its active principles. The present systematic review is based on 117 full‐text articles. Our review highlights (1) the need to apply complementary methodologies and consolidate protocols to quantify antimicrobial activity (2) to characterize antimicrobial agents in honeys of Heterotrigona itama, Tetragonisca angustula, and Melipona beecheii, and; (3) to search for compounds against multidrug‐resistant and/or biofilm‐forming pathogens. Moreover, we performed a meta‐analysis of 29 articles with quantitative data using diffusion assays and minimum inhibitory concentration (MIC). Staphylococcus aureus and Escherichia coli were the most evaluated pathogens, evidencing 221 and 149 antimicrobial activity assays against honey samples of 36 and 24 stingless bee species, respectively. The highest pooled mean in diffusion assays against S. aureus showed a difference between methicillin‐resistant S. aureus (MRSA) and non‐MRSA strains {14.43 [95% confidence interval (CI): 12.16–16.71] mm vs. 11.55 (95% CI: 10.22–12.87) mm}, whereas E. coli showed pooled means of 9.09 (95% CI: 7.93–10.25) mm. Regarding MIC expressed as volume/volume percentage (%, v/v), the estimated inhibitory concentrations were 7.89 (95% CI: 3.94–11.83) mL/100 mL for MRSA and 5.60 (95% CI: 2.66–8.55) mL/100 mL considering all S. aureus strains. In contrast, the estimated MICs as weight/volume percentage (%, w/v) showed 15.00 [(95% CI: 12.84–17.16) g/100 mL for S. aureus and 16.17 (95% CI: 5.78–26.55) g/100 mL for E. coli. However, honey remains an unexplored source of antimicrobial molecules.

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“…Infantis to thrive in various environments. Salmonella Infantis has been reported to be the most common serotype in live chickens and their byproducts ( Salazar et al, 2019 ; Mejía et al, 2021 ). Although human isolates were not included in this study, S .…”

Section: Discussionmentioning

confidence: 99%

Investigating the dynamics of Salmonella contamination in integrated poultry companies using a whole genome sequencing approach

Medina-Santana

¹

,

Ortega-Paredes

²

,

Janon

³

et al. 2022

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The study of non-typhoid Salmonella in broiler integrations has been limited by the resolution of typing techniques. Although serotyping of Salmonella isolates is used as a traditional approach, it is not of enough resolution to clearly understand the dynamics of this pathogen within poultry companies. The aim of this research was to investigate the epidemiology and population dynamics of Salmonella serotypes in 2 poultry integrations using a whole genome sequencing approach. Two hundred and forty-three Salmonella isolates recovered from the broiler production chain of 2 integrated poultry companies were whole genome sequenced and analyzed with dedicated databases and bioinformatic software. The analyses of sequences revealed that S . Infantis was the most frequent serotype (82.3%). Most isolates showed a potential for resistance against medically important antibiotics and disinfectants. Furthermore, 97.5% of isolates harbored the pESI-like mega plasmid, that plays an important role in the global dissemination of AMR. SNP tree analysis showed that there were clones that are niche-specific while other ones were distributed throughout the broiler production chains. In this study, we demonstrated the potential of whole genome sequencing analysis for a comprehensive understanding of Salmonella distribution in integrated poultry companies. Data obtained with these techniques allow determination of the presence of genetic factors that play an important role in the environmental fitness and pathogenicity of Salmonella .

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High Occurrence of MultiresistantSalmonellaInfantis in Retail Meat in Ecuador

Cited by 11 publications

References 27 publications

Characterization of a pESI-like plasmid and analysis of multidrug-resistant Salmonella enterica Infantis isolates in England and Wales

Characterization of a pESI-like plasmid and analysis of multidrug-resistant Salmonella enterica Infantis isolates in England and Wales

Antimicrobial activity of stingless bee honey (Tribe: Meliponini) on clinical and foodborne pathogens: A systematic review and meta‐analysis

Investigating the dynamics of Salmonella contamination in integrated poultry companies using a whole genome sequencing approach

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