High occurrence of antibiotic resistance and biofilm-formation among Stenotrophomonas maltophilia isolated from a tertiary hospital in Southwest of Iran

Mohagheghzadeh, Neda; Hashemizadeh, Zahra; Khashei, Reza; Kholdi, Soudeh; Mohebi, Samane; Motamedifar, Mohammad

doi:10.1016/j.genrep.2020.100827

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…In China and Egypt, the frequency of the smf -1 gene in S. maltophilia isolates was as high as 100% and 90%, respectively [ 28 , 32 ]. Unlike our study, a low level of the smf -1 gene was seen in the study conducted by Mohagheghzadeh et al [ 35 ]. This difference might be due to time of study.…”

Section: Discussioncontrasting

confidence: 97%

The Relationship between the Biofilm Genes and Antibiotic Resistance in Stenotrophomonas maltophilia

Sameni,

Hajikhani,

Hashemi

et al. 2023

International Journal of Microbiology

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Objectives. Today, Stenotrophomonas maltophilia (S. maltophilia) is a major opportunistic pathogen among hospitalized or immunocompromised patients. Antibiotic-resistant clinical isolates are increasing in several parts of the world. Various antibiotic-resistance and biofilm-forming genes are identified in this bacterium. Its capacity to form biofilms is an important virulence factor that may impact antibiotic-resistance patterns. In the current study, we evaluated the biofilm-formation capacity, antibiotic-resistance profile, and prevalence of biofilm-forming genes as well as antibiotic resistance genes among S. maltophilia isolates. Materials and Methods. In this cross-sectional study, 94 clinical S. maltophilia isolates were recovered from four tertiary-care hospitals in Iran between 2021 and 2022. The presence of the selected antibiotic-resistance genes and biofilm-forming genes was examined by polymerase chain reaction (PCR). The ability of biofilm formation was examined by microtiter plate assay. The Kirby–Bauer disc diffusion method was used to evaluate the trimethoprim-sulfamethoxazole (TMP-SMX), levofloxacin, and minocycline resistance. Results. S. maltophilia is mainly isolated from bloodstream infections. Notably, 98.93% of isolates were biofilm producers, of which 19.35%, 60.22%, and 20.43% produced strong, moderate, and weak biofilm, respectively. The frequency of biofilm genes was 100%, 97.88%, 96.80%, and 75.53% for spgM, rmlA, smf-1, and rpfF, respectively. Isolates with the genotype of smf-1+/rmlA+/spgM+/rpfF+ were mostly strong biofilm producers. Among the antibiotic-resistance genes, the Smqnr, L1, and sul1 had the highest prevalence (76.59%, 72.34%, and 64.89), respectively. Antimicrobial susceptibility evaluation showed 1.06%, 3.19%, and 6.3% resistance to minocycline, TMP-SMX, and levofloxacin. Conclusion. The results of the current study demonstrated that S. maltophilia isolates differ in biofilm-forming ability. Moreover, smf-1, rmlA, and spgM genes were presented in all strong biofilm producers. Although the overall resistance rate to the evaluated antibiotics was high, there was no statistically significant relation between antibiotic resistance and the type of biofilm.

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Section: Discussioncontrasting

confidence: 97%

The Relationship between the Biofilm Genes and Antibiotic Resistance in Stenotrophomonas maltophilia

Sameni,

Hajikhani,

Hashemi

et al. 2023

International Journal of Microbiology

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“…Recent evidence indicates that protease activity and biofilm-forming ability are vital pathogenic factors and potential spoilage determinants of S. maltophilia [10,16,17]. In a clinical study, extracellular proteases of S. maltophilia were proven to degrade human proteins and contribute to protease-mediated innate immune dysfunction in cystic fibrosis, tissue damage, and inflammation in patients' lungs [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Co-regulation of Thermosensor Pathogenic Factors by C-di-GMP-Related Two-Component Systems and a cAMP Receptor-like Protein (Clp) in Stenotrophomonas maltophilia

Ding,

Liao,

Wang

2024

Foods

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Stenotrophomonas maltophilia is a major threat to the food industry and human health owing to its strong protease production and biofilm formation abilities. However, information regarding regulatory factors or potential mechanisms is limited. Herein, we observed that temperature differentially regulates biofilm formation and protease production, and a cAMP receptor-like protein (Clp) negatively regulates thermosensor biofilm formation, in contrast to protease synthesis. Among four c-di-GMP-related two-component systems (TCSs), promoter fusion analysis revealed that clp transcription levels were predominantly controlled by LotS/LotR, partially controlled by both RpfC/RpfG and a novel TCS Sm0738/Sm0737, with no obvious effect caused by Sm1912/Sm1911. Biofilm formation in Δclp and ΔTCSs strains suggested that LotS/LotR controlled biofilm formation in a Clp-mediated manner, whereas both RpfC/RpfG and Sm0738/Sm0737 may occur in a distinct pathway. Furthermore, enzymatic activity analysis combined with c-di-GMP level indicated that the enzymatic activity of c-di-GMP-related metabolism proteins may not be a vital contributor to changes in c-di-GMP level, thus influencing physiological functions. Our findings elucidate that the regulatory pathway of c-di-GMP-related TCSs and Clp in controlling spoilage or the formation of potentially pathogenic factors in Stenotrophomonas expand the understanding of c-di-GMP metabolism and provide clues to control risk factors of S. maltophilia in food safety.

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“…Pesquisas envolvendo RmlAs ocorrem desde 1997, e, mesmo assim, novas descobertas ainda são reportadas sobre estas enzimas. Achados recentes indicam a presença do gene rmlA na maioria dos isolados produtores de biofilme em Stenotrophomonas maltophilia, [71][72][73] e a RmlA, em conjunto com a RmlC e RmlD, configuram entre as nove proteínas mais significativas no fator de virulência de Klebsiella pneumoniae ESBL (Extended-spectrum β-lactamases). 74 Dados como estes motivam avanços nos estudos estruturais e funcionais sobre a via de síntese de L-ramnose.…”

Section: Rmla E a Síntese De Ramnoseunclassified

>i<Enterococcus faecalis>/i<: >i<Cluster>/i< epa

Clementino

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Como ninguém chega a lugar nenhum sozinho, aqui está minha lista de agradecimento às pessoas e agências de fomento à pesquisa acadêmica, sem as quais eu definitivamente não teria escrito esta tese. Agradecer é só o que me resta.Agradeço à Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) pelo auxílio financeiro, processo nº 2017/24901-8, assim como ao CNPq e CAPES. Agradeço também aos funcionários da Universidade de São Paulo de São Carlos pela gestão e manutenção do local onde desenvolvi minha pesquisa.Agradeço ao professor Leonardo de Boni por sempre estar aberto a me ajudar na área de fotônica, além de ser uma pessoa muito agradável e simpática para um roqueiro, ao Prof. Dr. Francisco Guimarães pela ajuda com medidas confocais, à técnica Dra Andressa Patricia Alves Pinto por sua solicitude, os experimentos envolvendo o azul de molibdênio não ocorreriam sem ela me fornecer 3g do molibdato de amônio presente no Grupo de Biofísica. Agradeço ao Dr. Vinícius Ramos, por, dentre as discussões experimentais, sugerir a técnica de detecção de fosfato a partir do azul de molibdênio, à Dr. Luana Mourão, por sua disposição em ajudar todos os alunos do professor Alessandro durante a transição entre o Grupo de Biologia Molecular para o Grupo de Cristalografia. Muito obrigada.

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High occurrence of antibiotic resistance and biofilm-formation among Stenotrophomonas maltophilia isolated from a tertiary hospital in Southwest of Iran

Cited by 5 publications

References 23 publications

The Relationship between the Biofilm Genes and Antibiotic Resistance in Stenotrophomonas maltophilia

The Relationship between the Biofilm Genes and Antibiotic Resistance in Stenotrophomonas maltophilia

Co-regulation of Thermosensor Pathogenic Factors by C-di-GMP-Related Two-Component Systems and a cAMP Receptor-like Protein (Clp) in Stenotrophomonas maltophilia

>i<Enterococcus faecalis>/i<: >i<Cluster>/i< epa

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