High number of transplanted stem cells improves myocardial recovery after AMI in a porcine model

Alestalo, Kirsi; Korpi, Riikka; Mäkelä, Jussi; Lehtonen, Siri; Mäkelä, Tuomas; Yannopoulos, Fredrik; Ylitalo, Kari; Haapea, Marianne; Juvonen, Tatu; Anttila, Vesa; Lappi‐Blanco, Elisa; Sequeiros, Roberto Blanco; Lehenkari, Petri

doi:10.3109/14017431.2015.1018311

Cited by 12 publications

(10 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enormous research efforts have been focused on increasing the repair and regeneration of functional myocardial tissue, including approaches based on the administration of cells with regenerative capacity (cellular cardiomyoplasty) (Orlic et al , ; Kuhbier et al , ; Hoke et al , ; Behfar et al , ; Taylor and Robertson, ; Taylor, ). However, although preliminary reports in rodents and pigs have provided proof‐of‐concept data for most methods of regenerating damaged cardiac tissue (Tomita et al , ; Barbash et al , ; Bayes‐Genis et al , ; Alestalo et al , ; Song et al , 2014), the clinical results are not as solid or conclusive (Assmus et al , ; Cleland et al , : Strauer and Steinhoff, ; Roura et al ., ; Gyöngyösi et al , ). Major challenges are posed by the requirements for an efficient delivery system and the survival of large numbers of cells within the injured heart (Chachques et al , ; Soler‐Botija et al , ).…”

Section: Fibrin: Back To the Futurementioning

confidence: 99%

Fibrin, the preferred scaffold for cell transplantation after myocardial infarction? An old molecule with a new life

Roura

Gálvez‐Montón

Bayés‐Genís

2016

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Fibrin is a topical haemostat, sealant and tissue glue, which consists of concentrated fibrinogen and thrombin. It has broad medical and research uses. Recently, several studies have shown that engineered patches comprising mixtures of biological or synthetic materials and progenitor cells showed therapeutic promise for regenerating damaged tissues. In that context, fibrin maintains cell adherence at the site of injury, where cells are required for tissue repair, and offers a nurturing environment that protects implanted cells without interfering with their expected benefit. Here we review the past, present and future uses of fibrin, with a focus on its use as a scaffold material for cardiac repair. Fibrin patches filled with regenerative cells can be placed over the scarring myocardium; this methodology avoids many of the drawbacks of conventional cell-infusion systems. Advantages of using fibrin also include extraction from the patient's blood, an easy readjustment and implantation procedure, increase in viability and early proliferation of delivered cells, and benefits even with the patch alone. In line with this, we discuss the numerous preclinical studies that have used fibrin-cell patches, the practical issues inherent in their generation, and the necessary process of scaling-up from animal models to patients. In the light of the data presented, fibrin stands out as a valuable biomaterial for delivering cells to damaged tissue and for promoting beneficial effects. However, before the fibrin scaffold can be translated from bench to bedside, many issues must be explored further, including suboptimal survival and limited migration of the implanted cells to underlying ischaemic myocardium. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Section: Fibrin: Back To the Futurementioning

confidence: 99%

Fibrin, the preferred scaffold for cell transplantation after myocardial infarction? An old molecule with a new life

Roura

Gálvez‐Montón

Bayés‐Genís

2016

J Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…Acute myocardial infarction (AMI) is the most common cause of death worldwide ( 1 ) and current therapeutic strategies aim to establish revascularization ( 2 ). Administration of bone marrow cells has emerged as a potential candidate for cardiac tissue repair or as a paracrine modulator of healing after AMI in both non-clinical ( 3 – 6 ) and clinical studies ( 7 , 8 ), though the clinical results remain controversial.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, rapid and validated in vivo methods are needed for cellular labeling and visualization of transplanted cells. Most imaging related stem cell studies have employed bone marrow cells due to their ease of harvest, multi-lineage potential ( 9 – 11 ), immunological advantages, and potential therapeutic effect in AMI ( 3 – 6 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Two novel direct SPIO labels and in vivo MRI detection of labeled cells after acute myocardial infarct

Korpi

Alestalo

Ruuska

et al. 2017

Acta Radiologica Open

Self Cite

View full text Add to dashboard Cite

BackgroundAcute myocardial infarction (AMI) is a leading cause of morbidity and mortality worldwide. Cellular decay due hypoxia requires rapid and validated methods for possible therapeutic cell transplantation.PurposeTo develop direct and rapid superparamagnetic iron oxide (SPIO) cell label for a large-animal model and to assess in vivo cell targeting by magnetic resonance imaging (MRI) in an experimental AMI model.Material and MethodsBone marrow mononuclear cells (BMMNCs) were labeled with SPIO particles using two novel direct labeling methods (rotating incubation method and electroporation). Labeling, iron incorporation in cells and label distribution, cellular viability, and proliferation were validated in vitro. An AMI porcine model was used to evaluate the direct labeling method (rotating incubation method) by examining targeting of labeled BMMNCs using MRI and histology.ResultsLabeling (1 h) did not alter either cellular differentiation potential or viability of cells in vitro. Cellular relaxation values at 9.4 T correlated with label concentration and MRI at 1.5 T showing 89 ± 4% signal reduction compared with non-labeled cells in vitro. In vivo, a high spatial correlation between MRI and histology was observed. The extent of macroscopic pathological myocardial changes (hemorrhage) correlated with altered function detected on MRI.ConclusionWe demonstrated two novel direct SPIO labeling methods and demonstrated the feasibility of clinical MRI for monitoring targeting of the labeled cells in animal models of AMI.

show abstract

“…A more marked improvement of EF after intracoronary infusion of BMMNCs appears in patients with the most severe impairment of EF on admission [ 14 ]. In our pre-clinical study, the number of transplanted BMMNC in myocardium seem to associate with the improvement of EF after AMI [ 15 ]. However, we were unable to describe the exact mechanism underlying the better outcome.…”

Section: Introductionmentioning

confidence: 99%

Bone Marrow Mononuclear Cell Transplantation Restores Inflammatory Balance of Cytokines after ST Segment Elevation Myocardial Infarction

et al. 2015

Self Cite

View full text Add to dashboard Cite

BackgroundAcute myocardial infarction (AMI) launches an inflammatory response and a repair process to compensate cardiac function. During this process, the balance between proinflammatory and anti-inflammatory cytokines is important for optimal cardiac repair. Stem cell transplantation after AMI improves tissue repair and increases the ventricular ejection fraction. Here, we studied in detail the acute effect of bone marrow mononuclear cell (BMMNC) transplantation on proinflammatory and anti-inflammatory cytokines in patients with ST segment elevation myocardial infarction (STEMI).MethodsPatients with STEMI treated with thrombolysis followed by percutaneous coronary intervention (PCI) were randomly assigned to receive either BMMNC or saline as an intracoronary injection. Cardiac function was evaluated by left ventricle angiogram during the PCI and again after 6 months. The concentrations of 27 cytokines were measured from plasma samples up to 4 days after the PCI and the intracoronary injection.ResultsTwenty-six patients (control group, n = 12; BMMNC group, n = 14) from the previously reported FINCELL study (n = 80) were included to this study. At day 2, the change in the proinflammatory cytokines correlated with the change in the anti-inflammatory cytokines in both groups (Kendall’s tau, control 0.6; BMMNC 0.7). At day 4, the correlation had completely disappeared in the control group but was preserved in the BMMNC group (Kendall’s tau, control 0.3; BMMNC 0.7).ConclusionsBMMNC transplantation is associated with preserved balance between pro- and anti-inflammatory cytokines after STEMI in PCI-treated patients. This may partly explain the favorable effect of stem cell transplantation after AMI.

show abstract

High number of transplanted stem cells improves myocardial recovery after AMI in a porcine model

Abstract: BMMNC injection after AMI improved cardiac function. Quantity of transplanted BMMNCs correlated with the improvement in cardiac function after AMI.

Cited by 12 publications

References 27 publications

Fibrin, the preferred scaffold for cell transplantation after myocardial infarction? An old molecule with a new life

Fibrin, the preferred scaffold for cell transplantation after myocardial infarction? An old molecule with a new life

Two novel direct SPIO labels and in vivo MRI detection of labeled cells after acute myocardial infarct

Bone Marrow Mononuclear Cell Transplantation Restores Inflammatory Balance of Cytokines after ST Segment Elevation Myocardial Infarction

Contact Info

Product

Resources

About