High Negative Interference Over Short Segments of the Genetic Structure of Bacteriophage T4

Chase, Martha; Doermann, A. H.

doi:10.1093/genetics/43.3.332

Cited by 195 publications

(31 citation statements)

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“…The repair process may be analogous to that already described for an in uitro system by RICHARDSON, LEHMAN and KORNBERG ( 1964). The region over which cotransfer of markers in transformation can be detected is at most about 1 to 2 percent of the whole genome and thus corresponds in size to the region of effective pairing postulated by PRITCHARD (1955PRITCHARD ( , 1960a, CHASE and DOERMANN (1958), and others to explain high negative interference in gene tic fine-structure analysis. Thus, if a hybrid integrated region in transformation corresponds to such a region of effective pairing, our data are not in conflict with the elegant experiments of MESEUON andpersonal communication), andKELLENBERGER, et al (1961), suggesting that in phage , i recombination involves breakage and rejoining of double-stranded molecules.…”

Section: Discussionmentioning

confidence: 88%

Biochemical and Genetic Studies of Integration and Recombination in Bacillus Subtilis Transformation

1964

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Section: Discussionmentioning

confidence: 88%

Biochemical and Genetic Studies of Integration and Recombination in Bacillus Subtilis Transformation

1964

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“…This marker did not interfere with the scoring of mottled plaques (plaques containing both r and I+ phage). No detectable differences between any of the rII mutants used in this study were noted with regard to the transmission coefficient, which under the conditions employed, was characteristically between and 5 x (The transmission coefficient is the proportion of rII-infected K12 ( h ) complexes which yielded progeny (BENZER 1955) used by CHASE and DOERMANN ( 1958) , as it gives a lower transmission coefficient.…”

Section: Materials a N D Methodsmentioning

confidence: 81%

“…These mutants are identified with numbers greater than 100, e.g., r14,. The relative map locations of their markers have been given by CHASE and DOERMANN (1958).…”

Section: Materials a N D Methodsmentioning

confidence: 99%

“…This was advantageous in the experiments to be described. The media used and the procedure for preparing phage stocks were those described by CHASE and DOERMANN (1958).…”

Section: Materials a N D Methodsmentioning

confidence: 99%

“…

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confidence: 99%

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Phenotypic Properties of Heterozygotes in the Bacteriophage T4

Edgar

1958

Genetics

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rII region of the genome of the bacteriophage T4 has been particularly (CHASE and DOERMANN 1958), and gene function ( KRIEG 195 7). This is largely due to the differential response of phage mutant and nonmutant in this region to the bacterium Escherichia coli K12 (A). While rII+ phage may infect K l 2 (A) with a subsequent liberation of progeny, rII phage absorb to and kill K12 (A), but the complex does not produce any infectious progeny.By using mixed infections with different combinations of phage genotypes, BENZER (1955) was able to show that the rII region of the T4 genome consists of two adjacent but autonomously functioning segments. These segments he designated the A and B cistrons (BENZER 1957). He found that K12 (A) releases no phage when simultaneously infected with two different rA or with two different rB mutants, whereas infections of K12 (A) with both an rA and an rB mutant do result in the production of phage progeny (BENZER 1955).It therefore appears that both an unmutated A cistron and an unmutated B cistron must be present in an infected K12 (A) bacterium for the production of mature phage progeny. Since the genetic material of phage heterozygotes might have a structural organization differing from the presumed "diploidy" of a mixed infection, it is of interest to know if both structural conditions display similar phenotypic properties.From crosses of T2 involving a variety of markers, HERSHEY and CHASE ( 1951 ) found that two percent of the progeny particles recovered were heterozygous, i.e., showed subsequent segregation for a given pair of alleles. A heterozygote rarely segregates for more than one pair of alleles, except in cases of close linkage. Heterozygous rII markers in T4 also show this type of segregation pattern, and likewise are recognizably diploid only for short regions of the genome (DOER- MANN and KATAJA 1957). LEVINTHAL (1954) has presented evidence that the heterozygotes are intermediate stages in recombinant formation and that they are T useful HE for the investigation of mutation (BENZER 1955(BENZER ,1957, recombination 1 Submitted in partial fulfillment of the requirements for the degree Doctor of Philosophy to

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Coefficient of Coincidence

2004

Encyclopedic Dictionary of Genetics, Genomics and Proteomics

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High Negative Interference Over Short Segments of the Genetic Structure of Bacteriophage T4

Cited by 195 publications

References 5 publications

Biochemical and Genetic Studies of Integration and Recombination in Bacillus Subtilis Transformation

Biochemical and Genetic Studies of Integration and Recombination in Bacillus Subtilis Transformation

Phenotypic Properties of Heterozygotes in the Bacteriophage T4

Coefficient of Coincidence

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