High Molecular Weight Targets and Treatments Using Microdialysis

Clough, Geraldine F.; Stenken, Julie A.; Church, Martin K.

doi:10.1007/978-1-4614-4815-0_13

Cited by 2 publications

(2 citation statements)

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“…The technique involves using catheters or probes with semi-permeable membranes and microliter-per-minute perfusion flow rates to sample from living tissues. Microdialysis sampling of cytokines has been reviewed [281, 282]. …”

Section: In Vivo Methodsmentioning

confidence: 99%

Bioanalytical chemistry of cytokines – A review

Stenken

Poschenrieder

2015

Analytica Chimica Acta

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237

172

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Cytokines are bioactive proteins produced by many different cells of the immune system. Due to their role in different inflammatory disease states and maintaining homeostasis, there is enormous clinical interest in the quantitation of cytokines. The typical standard methods for quantitation of cytokines are immunoassay-based techniques including enzyme-linked immusorbent assays (ELISA) and bead-based immunoassays read by either standard or modified flow cytometers. A review of recent developments in analytical methods for measurements of cytokine proteins is provided. This review briefly covers cytokine biology and the analysis challenges associated with measurement of these biomarker proteins for understanding both health and disease. New techniques applied to immunoassay-based assays are presented along with the uses of aptamers, electrochemistry, mass spectrometry, optical resonator-based methods. Methods used for elucidating the release of cytokines from single cells as well as in vivo collection methods are described.

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Section: In Vivo Methodsmentioning

confidence: 99%

Bioanalytical chemistry of cytokines – A review

Stenken

Poschenrieder

2015

Analytica Chimica Acta

Self Cite

237

172

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show abstract

“…We are not aware of any microdialysis studies using membranes permeable to proteins larger than 3000 kDa. The perfusion across the membrane is not only determined by membrane pore size, but also by binding of proteins to tubing, other proteins and to the membrane, mass transport resistance and the diffusive properties of proteins and tissue structure (Clough et al ., ). However, it was most likely not due to steric hindrance or other limitations in perfusion, but to concentrations<LLQ that we did not detect the majority of biomarkers in situ .…”

Section: Discussionmentioning

confidence: 97%