High Molecular Weight RNA in a Murine Leukaemia Virus Helper-independent Strain of Moloney Sarcoma Virus

Vidrine, J. G.; Harewood, Ken R.; Bulfone, Laurette M.; Higdon, Carla R.; Mayyasi, Sami A.

doi:10.1099/0022-1317-20-2-239

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…scribed in the mouse cells nonproductively transformed by the Moloney isolate of MSV are smaller than those present in M-MLV-producing cells. In fact, the size of virus-specific RNA in non-virus-producing cells is almost identical to that found in S+Lvirions possessing the M-MSV genome (9,14). In addition, the Ki-MSV RNA subunit is reported to be smaller than the Ki-MLV RNA subunit (7).…”

Section: Figmentioning

confidence: 67%

Intracellular Viral RNA Species in Mouse Cells Nonproductively Transformed by the Murine Sarcoma Virus

Tsuchida

Green

1974

J Virol

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The size and quantity of virus-specific RNA in five non-virus-producing mouse cells transformed by the Moloney isolate of murine sarcoma virus (MSV) was determined. Hybridization of RNA from transformed cells with the [3H]DNA product of the RNA-directed DNA polymerase of the murine sarcoma-leukemia virus was used to detect and quantitate virus-specific RNA. The amount of virus-specific RNA in non-virus-producing cells was less than one-sixth of that found in virus-producing cells. A striking correlation was found between the amount of intracellular virus-specific RNA and the degree of agglutination by conconavalin A previously reported for the four non-virus-producing NIH/3T3 cell lines (Salzberg and Green, 1974). A major RNA subunit sedimenting at 26 to 28S was detected in all five MSV-transformed non-virus-producing cells. This could represent the RNA genome of defective MSV.

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Section: Figmentioning

confidence: 67%