High molecular weight glutenin subunit variation in the Sitopsis section of Aegilops. Implications for the origin of the B genome of wheat

Fernández-Calvı́n, Begoña; Orellana, Juan

doi:10.1038/hdy.1990.117

Cited by 35 publications

(13 citation statements)

References 32 publications

(31 reference statements)

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“…In Elytrigia elongata and Dasypyrum villosum, HMW glutenin subunits are found to be encoded by genes located on chromosome 1E and 1V, respectively (Lawrence and Shepherd 1981;Dvorµk et al 1986;Montebove et al 1987;De Pace et al 1988;Blanco et al 1991;Zhong and Qualset 1993). In the Aegilops genus, SDS-PAGE studies reveal that many species encode HMW glutenin subunits and that the expression of these subunits may be controlled by chromosomal loci similar to Glu-1 (Lawrence and Shepherd 1981;Fernµndez-Calvín and Orellana 1990;Peaea et al 1991;Urbano et al 1993;William et al 1993;Mackie et al 1996a;Wan et al 2000;Xie et al 2001;Pflüger et al 2001). The coding sequences for several Aegilops HMW glutenin subunits have also been isolated and characterized (Mackie et al 1996b;Xie et al 2001;Wan et al 2002).…”

Section: Introductionmentioning

confidence: 97%

Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species

et al. 2003

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Considerable progress has been made in understanding the structure, function and genetic regulation of high-molecular-weight (HMW) glutenin subunits in hexaploid wheat. In contrast, less is known about these types of proteins in wheat related species. In this paper, we report the analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species, Aegilops umbellulata (UU) and Aegilops caudata (CC). SDS-PAGE analysis demonstrated that, for each of the four Ae. umbellulata accessions, there were two HMW glutenin subunits (designated here as 1Ux and 1Uy) with electrophoretic mobilities comparable to those of the x- and y-type subunits encoded by the Glu-D1 locus, respectively. In our previous study involving multiple accessions of Ae. caudata, two HMW glutenin subunits (designated as 1Cx and 1Cy) with electrophoretic mobilities similar to those of the subunits controlled by the Glu-D1 locus were also detected. These results indicate that the U genome of Ae. umbellulata and the C genome of Ae. caudata encode HMW glutenin subunits that may be structurally similar to those specified by the D genome. The complete open reading frames (ORFs) coding for x- and y-type HMW glutenin subunits in the two diploid species were cloned and sequenced. Analysis of deduced amino acid sequences revealed that the primary structures of the x- and y-type HMW glutenin subunits of the two Aegilops species were similar to those of previously published HMW glutenin subunits. Bacterial expression of modified ORFs, in which the coding sequence for the signal peptide was removed, gave rise to proteins with electrophoretic mobilities identical to those of HMW glutenin subunits extracted from seeds, indicating that upon seed maturation the signal peptide is removed from the HMW glutenin subunit in the two species. Phylogenetic analysis showed that 1Ux and 1Cx subunits were most closely related to the 1Dx type subunit encoded by the Glu-D1 locus. The 1Uy subunit possessed a higher level of homology to the 1Dy-type subunit compared with the 1Cy subunit. In conclusion, our study suggests that the Glu-U1 locus of Ae. umbellulata and the Glu-C1 locus of Ae. caudata specify the expression of HMW glutenin subunits in a manner similar to the Glu-D1 locus. Consequently, HMW glutenin subunits from the two diploid species may have potential value in improving the processing properties of hexaploid wheat varieties.

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Section: Introductionmentioning

confidence: 97%

Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species

et al. 2003

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“…Variations at each Glu locus have been extensively studied in the past two decades, mainly due to their relationships with breadmaking quality (Payne 1987). On the other hand, storage protein polymorphisms are also useful genetic markers for crop origin and evolutionary studies (Fernandez-Calvin and Orellana 1990).…”

Section: Introductionmentioning

confidence: 99%

HMW and LMW glutenin alleles among putative tetraploid and hexaploid European spelt wheat (Triticum spelta L.) progenitors

Yan

Hsam

et al. 2003

Theor Appl Genet

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The allelic compositions of high- and low-molecular-weight subunits of glutenins (HMW-GS and LMW-GS) among European spelt ( Triticum spelta L.) and related hexaploid and tetraploid Triticum species were investigated by one- and two-dimensional polyacrylamide-gel electrophoresis (PAGE) and capillary electrophoresis (CE). A total of seven novel glutenin alleles (designated A1a*, B1d*, B1g*, B1f*, B1j*, D1a* at Glu-1 and A3h at the Glu-3 loci, respectively) in European spelt wheat were detected by SDS-PAGE, which were confirmed further by employing A-PAGE and CE methods. Particularly, two HMW-GS alleles, Glu-B1d* coding the subunits 6.1 and 22.1, and Glu-B1f* coding the subunits 13 and 22*, were found to occur in European spelt with frequencies of 32.34% and 5.11%, respectively. These two alleles were present in cultivated emmer (Triticum dicoccum), but they were not observed in bread wheat (Triticum aestivum L.). The allele Glu-B1g* coding for 13* and 19* subunits found in spelt wheat was also detected in club wheat (Triticum compactum L.). Additionally, two alleles coding for LMW-GS, Glu-A3h and Glu-B3d, occurred with high frequencies in spelt, club and cultivated emmer wheat, whereas these were not found or present with very low frequencies in bread wheat. Our results strongly support the secondary origin hypothesis, namely European spelt wheat originated from hybridization between cultivated emmer and club wheat. This is also confirmed experimentally by the artificial synthesis of spelt through crossing between old European emmer wheat, T. dicoccum and club wheat, T. compactum.

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“…The allele encoding the x-component only at the Glu-M1 locus was also identified in one accession of Aegilops comosa by Rodriguez-Quijano et al (2001), and in one accession of Aegilops biuncialis analyzed by Kozub et al (2011). Alleles coding for the x-type subunit only are common at Glu-A1 locus, encountered among alleles at the Glu-B1 locus and uncommon at the Glu-D1 locus in cultivated wheat (Payne and Lawrence 1983;Fernández-Calvín and Orellana 1990;Saponaro et al 1995). In this study, nineteen bands (HMW glutenin subunits) were detected result-ing in twenty-nine different patterns from the analyzed collection.…”

Section: Discussionmentioning

confidence: 99%

“…The HMW glutenin subunits are controlled by the complex Glu-1 loci present on the long arm of the group 1 homologous chromosomes of wheat and relatives which contains two tightly linked genes coding for a subunit of lower mobility and another of faster mobility, termed x-and y-type, respectively (Kozub et al 2011). Some genetic diversity studies based on proteins and DNA polymorphism have been carried out on Aegilops species, but more frequently on the diploid species that are the genome donors to polyploid Aegilops and Triticum species (Fernández-Calvín and Orellana 1990;Rodriguez-Quijano et al 2001;De Bustos and Jouve 2006;Sun et al 2006). There are few genetic diversity studies in Aegilops geniculata, except some works using RFLP and RAPD polymorphism (Zhang et al 1996;Monte et al 1999) or proteins (Bandou et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Polymorphism at High Molecular Weight Glutenin Subunits and Morphological Diversity ofAegilops geniculataRoth Collected in Algeria

Medouri

Bellil

Khelifi

2015

Cereal Research Communications

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A collection of 35 accessions of the tetraploid wild wheat Aegilops geniculata Roth (MM, UU) sampled in northern Algeria was evaluated for morphological and biochemical variability. Morphological and ecological analyses based on morphological traits and bioclimatic parameters, respectively, were assessed using principal component analysis (PCA). Accessions were differentiated by width characters, namely spike's width, and a weak relationship between morphological traits and ecological parameters was found. Polymorphism of high molecular weight (HMW) glutenin subunits was carried on by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Among accessions analyzed, 27 alleles were identified at the two loci Glu-M1 and Glu-U1: resulting in twenty-nine patterns and a nomenclature was proposed. Two alleles at the Glu-U1 locus expressed a new subunit with a slightly slower mobility than subunit 8. These results provide new information regarding the genetic variability of HMW glutenin subunits, as well as their usefulness in cultivated wheat quality improvement.

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High molecular weight glutenin subunit variation in the Sitopsis section of Aegilops. Implications for the origin of the B genome of wheat

Cited by 35 publications

References 32 publications

Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species

Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species

HMW and LMW glutenin alleles among putative tetraploid and hexaploid European spelt wheat (Triticum spelta L.) progenitors

Polymorphism at High Molecular Weight Glutenin Subunits and Morphological Diversity ofAegilops geniculataRoth Collected in Algeria

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