High molecular weight, but not total, CTRP3 levels are associated with serum triglyceride levels

Trogen, Greta; Alamian, Arsham; Peterson, Jonathan M.

doi:10.14814/phy2.14306

Cited by 1 publication

(5 citation statements)

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“…Our previous work demonstrated that CTRP3 is only found in human circulation as MMW or HMW isoforms [26]; this was also confirmed experimentally in the pediatric study population presented herein (S1 Fig). In addition, Trogen et al [26] found that the differences in the circulating oligomeric forms of CTRP3 had strong correlations with obesity and other metabolic variables, especially circulating triglyceride levels. Therefore, we hypothesized that the MMW and HMW oligometric CTRP3 will be correlated with metabolic variables, specifically circulating serum triglyceride levels.…”

Section: Plos Onesupporting

confidence: 87%

“…However, it has been speculated that the oligomer conformation of CTRP3 is important for its functional activity [19,22,40,41], and this has been rarely studied. To date only one other study has examined the oligomeric status of CTRP3 with human disease [26]. Trogen et al (2019) determined that all circulating CTRP3 in humans is found as either the MMW or HMW oligomer, with the trimer and monomer of CTRP3 being undetectable.…”

Section: Discussionmentioning

confidence: 99%

“…To date only one other study has examined the oligomeric status of CTRP3 with human disease [26]. Trogen et al (2019) determined that all circulating CTRP3 in humans is found as either the MMW or HMW oligomer, with the trimer and monomer of CTRP3 being undetectable. Further, the authors found that the oligomeric state of CTRP3 was significantly correlated to serum triglyceride levels, but not diabetic or obesity status [21].…”

Section: Discussionmentioning

confidence: 99%

“…Although CTRP3 is an approximately 26 kDa protein, when secreted CTRP3 forms higher order molecule structures such as a trimer (~78-90 kDa), a six-nine subunit oligomer also known as a middle molecular weight oligomer (MMW,~180-270 kDa), or as a high molecular weight oligomer (HMW, >300 kDa) [9]. Our previous work demonstrated that CTRP3 is only found in human circulation as MMW or HMW isoforms [26]; this was also confirmed experimentally in the pediatric study population presented herein (S1 Fig). In addition, Trogen et al [26] found that the differences in the circulating oligomeric forms of CTRP3 had strong correlations with obesity and other metabolic variables, especially circulating triglyceride levels.…”

Section: Plos Onementioning

confidence: 99%

“…However, there have been conflicting studies regarding the relationship between circulating CTRP3 levels and metabolic status. For example, CTRP3 has been reported to be elevated [24], not changed [25,26], or reduced with obesity [24,[27][28][29][30]. These conflicting data suggest that measuring total circulating levels of CTRP3 is insufficient for determining the role of CTRP3 in human health.…”

Section: Introductionmentioning

confidence: 99%

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CTRP3 and serum triglycerides in children aged 7-10 years

et al. 2020

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Introduction The prevalence of obesity-related disorders has been steadily increasing over the past couple of decades. Diseases that were once only detected in adults are now prevalent in children, such as hyperlipidemia. The adipose tissue-derived hormonal factor C1q TNF Related Protein 3 (CTRP3) has been linked to triglyceride regulation especially in animal models. However, the relationship between circulating CTRP3 levels and obesity-related disorders in human subjects is controversial. CTRP3 can circulate in different oligomeric complexes: trimeric (<100 kDa), middle molecular weight (100–300 kDa), and high molecular weight (HMW) oligomeric complexes (>300 kDa). Previous work has identified that it is not the total amount of CTRP3 present in the serum, but the specific circulating oligomeric complexes that appear to be indicative of the relationship between CTRP3 and serum lipids levels. However, this work has not been examined in children. Therefore, the purpose of this study was to compare the levels of different oligomeric complexes of CTRP3 and circulating lipid levels among young children (aged 7–10 years). Methods Morphometric data and serum samples were collected and analyzed from a cross-sectional population of 62 children of self-identified Hispanic origin from a community health center, between 2015 and 2016. Serum analysis included adiponectin, insulin, leptin, ghrelin, glucagon, C-reactive peptide, triglyceride, cholesterol, IL-6, TNF, and CTRP3. Correlation analyses were conducted to explore the relationships between CTRP3 and other biomarkers. Results Total CTRP3 concentrations were significantly positively correlated with total cholesterol and HDL cholesterol. Whereas, HMW CTRP3 was not significantly associated with any variable measured. Conversely, the middle molecular weight (MMW) CTRP3 was negatively correlated with triglycerides levels, and very low-density lipoprotein (VLDL), insulin, and body mass index (BMI). The negative correlations between MMW CTRP3 and triglycerides and VLDLs were particularly strong (r2 = -0.826 and -0.827, respectively). Conclusion Overall, these data indicate that the circulating oligomeric state of CTRP3 and not just total CTRP3 level is important for understanding the association between CTRP3 and metabolic diseases. Further, this work indicates that MMW CTRP3 plays an important role in triglyceride and VLDL regulation which requires further study.

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Section: Plos Onesupporting

confidence: 87%