High molecular polymorphism of the hypervariable region in the VP2 gene of Aleutian mink disease virus

Jakubczak, Andrzej; Kowalczyk, Marek; Kostro, K.; Horecka, Beata; Jeżewska-Witkowska, Grażyna

doi:10.4149/av_2016_04_354

Cited by 9 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our research, based on polymorphism in the sequence encoding the VP2 protein, showed eight closely related variants of the pathogen. High variability on a single farm has been confirmed by previous analyses conducted in Poland [27], as well as results reported by Canuti et al [28], who observed considerable variation in the pathogen resulting from the high stocking density on the farm. Polymorphism in the nucleotide sequence of the VP2 protein of the virus may translate into changes in its structure and functionality and thus result in a varied immune response in the host.…”

Section: Discussionsupporting

confidence: 81%

Breeding parameters on a mink farm infected with Aleutian mink disease virus following the use of methisoprinol

et al. 2019

View full text Add to dashboard Cite

Aleutian mink disease virus is one of the greatest threats to modern mink farming. The disease reduces fecundity and causes high mortality among kits. The aim of this study was to evaluate the effectiveness of methisoprinol in counteracting the effects of Aleutian disease, both by inhibiting replication of the virus and by mitigating the harmful effects of the disease on the fecundity and weight of infected animals. The study included 300 individuals with confirmed infection, divided according to antibody titres into three experimental groups, which received a 20% methisoprinol solution, and three control groups, which did not receive the immunostimulant. In the mink from the experimental groups, the number of copies of the genetic material of the virus in the spleens and lymph nodes was one order of magnitude lower than in the case of the control groups. Mink receiving the supplement also showed higher fecundity (on average 5.83 in the experimental groups and 4.83 in the control groups), and the weight of their offspring before slaughter was over 200 g higher. Given the lack of effective methods for immunoprophylaxis and treatment, methisoprinol supplementation can be an effective means of counteracting the effects of AMDV on persistently infected farms.Electronic supplementary materialThe online version of this article (10.1007/s00705-019-04375-x) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionsupporting

confidence: 81%

Breeding parameters on a mink farm infected with Aleutian mink disease virus following the use of methisoprinol

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The VP2 protein, containing a hypervariable region and epitopic regions, is largely responsible for the antigenic properties of the pathogen and its spectrum of infection [22,23]. In the case of amdoviruses, which include AMDV, the variation between isolates is particularly high even within individual farms [24].…”

Section: Discussionmentioning

confidence: 99%

A comparative molecular characterization of AMDV strains isolated from cases of clinical and subclinical infection

et al. 2018

View full text Add to dashboard Cite

The Aleutian mink disease virus (AMDV) is one of the most serious threats to modern mink breeding. The disease can have various courses, from progressive to subclinical infections. The objective of the study was to provide a comparative molecular characterization of isolates of AMDV from farms with a clinical and subclinical course of the disease. The qPCR analysis showed a difference of two orders of magnitude between the number of copies of the viral DNA on the farm with the clinical course of the disease (10) and the farm with the subclinical course (10). The sequencing results confirm a high level of homogeneity within each farm and variation between them. The phylogenetic analysis indicates that the variants belonging to different farms are closely related and occupy different branches of the same clade. The in silico analysis of the effect of differences in the sequence encoding the VP2 protein between the farms revealed no effect of the polymorphism on its functionality. The close phylogenetic relationship between the isolates from the two farms, the synonymous nature of most of the polymorphisms and the potentially minor effect on the functionality of the protein indicate that the differences in the clinical picture may be due not only to polymorphisms in the nucleotide and amino acid sequences, but also to the stage of infection on the farm and the degree of stabilization of the pathogen-host relationship.

show abstract

“…Given the resources of bioinformatic databases of AMDV sequences, it seems reasonable to select primers that fl ank the most frequently deposited sequences, in order to allow global analysis of the epidemiology of the virus. The primers used in our research have previously been used in single reactions in a study on phylogenetic analyses between AMDV isolates [28,15]. Phylogentic analysis based on the sequence coding NS1 protein confi rmed that obtained variants belong to the clade with the highly pathogenic strains Utah, and ADV-LN1-3 [29] and are distant from non-pathogenic AMDV-G, which forms separate clade.…”

Section: Discussionmentioning

confidence: 78%

“…High genetic variability is also observed in the case of the Aleutian virus, which belongs to the family Parvoviridae. There are reports of numerous variants occurring on a single farm [15], or even co-infections of one host with several variants [16]. Not only can high genetic variability cause changes in antigenic properties and the appearance of falsenegative results in serological methods [17], but due to the occurrence of mutations at primer binding sites, it can result in decreased effi cacy or even a lack of amplifi cation in diagnostics based on classic PCR.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Duplex PCR for Detection of Aleutian Disease Virus from Biological and Environmental Samples

2019

View full text Add to dashboard Cite

Aleutian disease is one of the most serious disease entities affecting mink farms. The disease causes significant economic losses in mink breeding countries. The aim of the study was to optimize a diagnostic test based on duplex PCR to enable detection of Aleutian disease virus in biological and environmental samples. Blood (n = 40) and spleen (n = 40) samples from animals with suspected infection, and swabs from cages in which infected animals were kept (n = 20) were used for analysis. DNA was isolated from the samples, followed by optimization of the duplex PCR reaction targeting sequences coding NS1 and VP2 proteins. The qPCR method was used to determine the sensitivity of the reaction. The specificity of the analysis was confirmed by the sequencing results. Optimized duplex PCR enabled detection of Aleutian Mink Disease Virus (AMDV) genetic material in biological and environmental samples. Testing of the sensitivity of the method indicated clear amplification for both primer pairs at 102 copies of viral DNA in a reaction. Sequencing confirmed the specificity of the reaction, which in the case of both primer pairs indicated an over 90% agreement between the isolates and the variants of the virus from the databases. The use of duplex PCR to detect two regions of the AMDV genome may increase the sensitivity and specificity of the method and significantly expand the possibilities of further analysis based on sequencing.

show abstract

High molecular polymorphism of the hypervariable region in the VP2 gene of Aleutian mink disease virus

Cited by 9 publications

References 23 publications

Breeding parameters on a mink farm infected with Aleutian mink disease virus following the use of methisoprinol

Breeding parameters on a mink farm infected with Aleutian mink disease virus following the use of methisoprinol

A comparative molecular characterization of AMDV strains isolated from cases of clinical and subclinical infection

Duplex PCR for Detection of Aleutian Disease Virus from Biological and Environmental Samples

Contact Info

Product

Resources

About