High-molecular-mass lipopolysaccharides are involved in Actinobacillus pleuropneumoniae adherence to porcine respiratory tract cells

Paradis, Sonia-Élaine; Dubreuil, Daniel; Rioux, Stéphane; Gottschalk, Marcelo; Jacques, Mario

doi:10.1128/iai.62.8.3311-3319.1994

Cited by 74 publications

(42 citation statements)

References 32 publications

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“…The field strain FMV87-682 showed only one peak of high fluorescence intensity after growth under iron-sufficient conditions (Fig. 3A), as we reported before [7], and also after growth under iron-restricted conditions (Fig. 3B).…”

Section: Relatwe Fluorescencesupporting

confidence: 81%

“…were incubated with either mAb against serotype 1 O-antigen or serotype 1 K-antigen, or mAb E5 directed against bacterial lipid A (kindly supplied by P. Dessain. Pfizer Canada, Inc., Pointe-Claire, Quebec, Canada) and analyzed by flow cytometry as described previously [7]. The flow analysis was performed with a FACStar flow cytometer (Becton Dickinson Immunocytometry Systems, San Jose, CA) equipped with a water-cooled 2 W argon ion laser operating at 488 nm and a 200 mW light output.…”

Section: Cells Of a Pleuropneumoniaementioning

confidence: 99%

“…The presence of these two cell subpopulations is probably not due to differences in capsulation since labelling with a mAb against the K-antigen resulted in only one population (peak), nor to different amounts of LPS since labelling with a mAb against the lipid A resulted in only one population. One possible explanation for the presence of the oberved two subpopulations might be the bimodal distribution of O-chain lengths in A. pleuropneumoniae LPS [7]; one subpopulation of cells expresses LPS with longer O-chains than the other subpopulation, and can thus bind more antibodies at their surface. Another possibility is that the observed cell subpopulations correspond to the two serotype 1 LPS subtypes (1A and 1B) recently proposed by Jolie et al [19].…”

Section: Relatwe Fluorescencementioning

confidence: 99%

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Examination of surface polysaccharides of Actinobacillus pleuropneumoniae serotype. 1 Grown under iron-restricted conditions

Paradis

1996

FEMS Microbiology Letters

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We investigated the expression of important Actinobacillus pleuropneumoniae surface polysaccharides, namely, capsular polysaccharides (CPS) and lipopolysaccharides (LPS), after growth under iron-restricted conditions. Iron restriction did not seem to affect the production of CPS, as determined by labelling with a monoclonal antibody (mAb) against the serotype 1 K-antigen and flow cytometry analysis, and also as determined by electron microscopy. SDS-PAGE revealed that the LPS profiles of these cells were also unaffected by iron restriction. Using flow cytometry analysis, however, we observed that binding of mAb against serotype 1 O-antigen was altered in cells of A. pleuropneumoniae serotype 1 reference strain (4074) grown under iron-restricted conditions. This strain exhibited two subpopulations with distinct patterns of reactivity with the mAb against the O-antigen. When strain 4074 was grown under iron-restricted conditions, a shift from one cell subpopulation (moderately fluorescent) to another cell subpopulation (highly fluorescent, thus binding more antibodies) was observed. Our results indicate that growth of A. pleuropneumoniae serotype 1 under iron-restricted conditions did not seem to affect CPS production, but might alter, at least for the reference strain, the expression of LPS.

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Section: Relatwe Fluorescencesupporting

confidence: 81%

Section: Cells Of a Pleuropneumoniaementioning

confidence: 99%

Section: Relatwe Fluorescencementioning

confidence: 99%

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Examination of surface polysaccharides of Actinobacillus pleuropneumoniae serotype. 1 Grown under iron-restricted conditions

Paradis

1996

FEMS Microbiology Letters

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“…Isolates with a smooth LPS pro¢le adhered in larger numbers to tracheal rings than isolates with a semirough LPS pro¢le. We then found, by using extracted LPS from serotypes 1 and 2, that the polysaccharidic part of this complex molecule, but not the lipidic part, was involved in binding to host cells [82]. We also showed in the latter study, using immunoelectron microscopy and £ow cytometry, that LPS were well exposed at the surface of this heavily encapsulated bacterium, an essential prerequisite for a bacterial adhesin.…”

Section: Actinobacillus Pleuropneumoniaementioning

confidence: 71%

Adhesin–receptor interactions in Pasteurellaceae

Jacques¹

1998

FEMS Microbiology Reviews

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The ability of bacteria to adhere to mucosal epithelium is dependent on the expression of adhesive molecules or structures, called adhesins, that allow attachment of the organisms to complementary molecules on mucosal surfaces, the receptors. Important human and animal pathogens are found among the Pasteurellaceae family which includes Haemophilus, Actinobacillus, and Pasteurella organisms. The purpose of this paper is to review the adhesin-receptor systems found in Pasteurellaceae, with an emphasis on recent developments in this specific area. Most of these organisms can employ multiple molecular mechanisms of adherence (or multiple adhesins) to initiate infection. Indeed, a wide variety of adhesins are expressed by members of the Pasteurellaceae, and different proteins (e.g. fimbriae, fibrils, outer membrane proteins) as well as polysaccharides (lipooligosaccharides, lipopolysaccharides, capsular polysaccharides) were clearly shown to play an important role in adherence. In many instances, these adhesins have proved to represent good vaccine candidates. Surprisingly, the receptors on host mucosal surfaces have yet been identified in very few cases.

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“…A core oligosaccharide region (R-core) covalently bridges a lipid anchored to the outer leaflet of the phospholipid bilayer (lipid A) with a side chain composed of a number of repeating oligosaccharide units (O-chain) (Wilkinson 1996;Nikaido 2003). LPS is thought to be an endotoxin in animal immune systems (Ulevitch and Tobias 1995;Raetz and Whitfield 2002), is a permeation barrier against exogenous lipophilic toxins (Labischinski et al 1985;Nikaido 2003) and provides membrane structural integrity (Skurnik et al 1999;Walsh et al 2000) and cellular adhesion or recognition (Paradis et al 1994;Shapiro et al 1997). However, some evidence suggests that LPSs in cyanobacteria are structurally and functionally different from well-studied proteobacteria (Hoiczyk and Hansel 2000;Snyder et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Monosaccharide composition of the outer membrane lipopolysaccharide and O-chain from the freshwater cyanobacterium Microcystis aeruginosa NIES-87

et al. 2012

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Aims Bacterial lipopolysaccharide (LPS) protruding from the outermost layer of the outer membrane is expected to play an important role in cell physiology by interacting with molecules in the extracellular milieu; however, the structural and functional characteristics of these components in cyanobacteria remain largely unknown. We isolated water‐soluble fractions of LPS and O‐chain from the bloom‐forming freshwater cyanobacterium Microcystis aeruginosa NIES‐87 and identified their monosaccharide compositions. Methods and Results SDS‐PAGE followed by silver staining demonstrated that the isolated total LPS was the smooth type with different numbers of repeating sugar units in the O‐chain region. GC/MS analysis after acid hydrolysis, reduction and acetylation treatments indicated that the neutral monosaccharide components of the total LPS include glucose, rhamnose, mannose, galactose and xylose (in decreasing order of weight percentage), while only glucose was detected in the purified O‐chain fraction. MALDI‐TOF MS analysis suggested that the O‐chain fraction is composed of repeating glucose and methylated glucose disaccharide units. Conclusions Our results indicate that the monosaccharide composition of M. aeruginosa O‐chain is relatively simple. Significance and Impact of the Study Although further studies are necessary, these findings provide fundamental information for understanding the structural and functional properties of cyanobacterial LPS and O‐chain.

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High-molecular-mass lipopolysaccharides are involved in Actinobacillus pleuropneumoniae adherence to porcine respiratory tract cells

Abstract: Actinobacilus pleuropneumoniae is the causative agent of porcine pleuropneumonia. The major adhesin ofA. pleuropneumoniae has been identified as the lipopolysaccharides (LPSs) (

Cited by 74 publications

References 32 publications

Examination of surface polysaccharides of Actinobacillus pleuropneumoniae serotype. 1 Grown under iron-restricted conditions

Examination of surface polysaccharides of Actinobacillus pleuropneumoniae serotype. 1 Grown under iron-restricted conditions

Adhesin–receptor interactions in Pasteurellaceae

Monosaccharide composition of the outer membrane lipopolysaccharide and O-chain from the freshwater cyanobacterium Microcystis aeruginosa NIES-87

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