High mobility group Box-1 inhibits cancer cell motility and metastasis by suppressing activation of transcription factor CREB and nWASP expression

Zuo, Zhenghong; Che, Xun; Wang, Yulei; Li, Bowen; Li, Jingxia; Dai, Wei; Lin, Charles P.; Huang, Chuanshu

doi:10.18632/oncotarget.2150

Cited by 24 publications

(26 citation statements)

References 49 publications

(56 reference statements)

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“…Conversely, exogenous HMGB1 significantly decreased apoptosis and increased both invasion and levels of p-NF-κB. Knockdown of HMGB1 in human lung cancer A549 cells significantly increased cell β-actin polymerization, cell skeleton formation, cancer cell migration and invasion in vitro, as well as metastasis in vivo 20. In human cutaneous squamous cell carcinoma, administration of HMGB1 to SCC13 cells caused an increase in cell migration in a time- and dose-dependent manner 28.…”

Section: Discussionmentioning

confidence: 99%

“…HMGB1 suppresses tumorigenesis by interacting with tumor suppressor genes such as p53, p73 and RB 16,17. Moreover, HMGB1 has complex effects on the hallmarks of cancer, such as limitless replicative potential, sustained angiogenesis, evasion of apoptosis, self-sufficiency in growth signals, insensitivity to growth inhibitors and tissue invasion and metastasis 18–20. However, the role of HMGB1 in the invasion and metastasis of endometrial cancer is unknown.…”

Section: Introductionmentioning

confidence: 99%

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HMGB1 is negatively correlated with the development of endometrial carcinoma and prevents cancer cell invasion and metastasis by inhibiting the process of epithelial-to-mesenchymal transition

Luan¹,

Ma²,

Wang³

et al. 2017

OTT

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High-mobility group box protein 1 (HMGB1), a nuclear protein that plays a significant role in DNA architecture and transcription, was correlated with the progression of some types of cancer. However, the role of HMGB1 in endometrial cancer cell invasion and metastasis remains unexplored. HMGB1 expression was initially assessed by immunohistochemistry and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in normal endometrial tissue and endometrial carcinoma tissue. High expressions of HMGB1 protein were detected in normal endometrial tissues; however, in endometrial cancer tissues, the expressions of HMGB1 were found to be very weak. Furthermore, HMGB1 expressions were negatively correlated with advanced stage and lymph node metastasis in endometrial cancer. Then by RT-qPCR, Western blot and immunocytochemistry, HMGB1 was also detected in primary cultured endometrial cells and four kinds of endometrial cancer cell lines (Ishikawa, HEC-1A, HEC-1B and KLE). We found that the expression of HMGB1 was much higher in normal endometrial cells than in endometrial cancer cells, and reduced expression levels of HMGB1 were observed especially in the highly metastatic cell lines. Using lentivirus transfection, HMGB1 small hairpin RNA was constructed, and this infected the lowly invasive endometrial cancer cell lines, Ishikawa and HEC-1B. HMGB1 knockdown significantly enhanced the proliferation, invasion and metastasis of endometrial cancer cells and induced the process of epithelial-to-mesenchymal transition. These results can contribute to the development of a new potential therapeutic target for endometrial cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HMGB1 is negatively correlated with the development of endometrial carcinoma and prevents cancer cell invasion and metastasis by inhibiting the process of epithelial-to-mesenchymal transition

Luan¹,

Ma²,

Wang³

et al. 2017

OTT

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“…After incubation in a humidified incubator at 37°C, 5% CO 2 atmosphere for 24 hr, non‐migrating or non‐invading cells were wiped using cotton swab according to manufacturer's instructions. Cell numbers were quantified and the images were captured under inverted microscope (Olympas, Center Valley, PA) as describe in our previous study …”

Section: Methodsmentioning

confidence: 99%

XIAP overexpression promotes bladder cancer invasion in vitro and lung metastasis in vivo via enhancing nucleolin‐mediated Rho‐GDIβ mRNA stability

Jin

et al. 2017

Intl Journal of Cancer

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Our recent studies demonstrate that X-linked inhibitor of apoptosis protein (XIAP) is essential for regulating colorectal cancer invasion. Here, we discovered that RhoGDIβ was a key XIAP downstream effector mediating bladder cancer (BC) invasion in vitro and in vivo. We found that both XIAP and RhoGDIβ expressions were consistently elevated in BCs of N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN)-treated mice in comparison to bladder tissues from vehicle-treated mice and human BCs in comparison to the paired adjacent normal bladder tissues. Knockdown of XIAP attenuated RhoGDIβ expression and reduced cancer cell invasion, whereas RhoGDIβ expression was attenuated in BBN-treated urothelium of RING-deletion knockin mice. Mechanistically, XIAP stabilized RhoGDIβ mRNA by its positively regulating nucleolin mRNA stability via Erks-dependent manner. Moreover, ectopic expression of GFP-RhoGDIβ in T24T(shXIAP) cells restored its lung metastasis in nude mice. Our results demonstrate that XIAP-regulated Erks/nucleolin/RhoGDIβ axis promoted BC invasion and lung metastasis.

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“…However, various genes such as posphoinositide‐specific phospholipase gamma 2 (PLCG2), PI3Ks, protein kinase C (PKC), and diacylglycerol kinase zeta (DGKZ) were selected as kernels in the gene regulatory network . In addition, HMGB1 suppressed phosphorylation, nuclear translocation and activation of CREB by inhibiting nuclear translocation of PKA catalytic subunit, and then impaired cancer cell motility …”

Section: Introductionmentioning

confidence: 99%

The role of high‐mobility group protein box 1 in lung cancer

Wu¹,

Chen²,

Gong

et al. 2018

J of Cellular Biochemistry

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High-mobility group protein box 1(HMGB1)is a ubiquitous highly conserved nuclear protein. Acting as a chromatin-binding factor, HMGB1 binds to DNA and plays an important role in stabilizing nucleosome formation, facilitating gene transcription, DNA repairing, inflammation, cell differentiation, and regulating the activity of steroid hormone receptors. Currently, HMGB1 is discovered to be related to development, progression, and targeted therapy of lung cancer, which makes it an attractive biomarker, and therapeutic target. This review aims to encapsulate the relationship between HMGB1 and lung cancer, suggesting that HMGB1 plays a pivotal role in initiation, development, invasion, metastasis, and prognosis of lung cancer.

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High mobility group Box-1 inhibits cancer cell motility and metastasis by suppressing activation of transcription factor CREB and nWASP expression

Cited by 24 publications

References 49 publications

HMGB1 is negatively correlated with the development of endometrial carcinoma and prevents cancer cell invasion and metastasis by inhibiting the process of epithelial-to-mesenchymal transition

HMGB1 is negatively correlated with the development of endometrial carcinoma and prevents cancer cell invasion and metastasis by inhibiting the process of epithelial-to-mesenchymal transition

XIAP overexpression promotes bladder cancer invasion in vitro and lung metastasis in vivo via enhancing nucleolin‐mediated Rho‐GDIβ mRNA stability

The role of high‐mobility group protein box 1 in lung cancer

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