High mitochondrial diversity in geographically widespread butterflies of Madagascar: A test of the DNA barcoding approach

Linares, Marjorie C.; Soto-Calderón, Iván Darío; Lees, David C.; Anthony, Nicola M.

doi:10.1016/j.ympev.2008.11.008

Cited by 79 publications

(88 citation statements)

References 83 publications

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“…The mean K2P distance observed The present mtDNA locus ATPase 8 and 6 genes studied showed almost similar genetic divergence (K2P) and phylogenetic patterns observed with the DNA barcoding locus COI, within the species P. denisonii [49]. However, the pattern of monophyletic association exhibited among different lineages within the species P. denisonii, with high branch support, and with an average of 10X greater divergence between than within each lineage ( Figure 5, Table 7) satisfies the basic concept of COI based DNA barcoding, for species identification and discovery [50][51][52], in identifying new species. The high level of genetic and morphologic diversity observed among the Denison's barb, distributed along the isolated habitats of the Western Ghats, make it a species complex, with a scope of at least four cryptic species within them, being discussed further.…”

Section: Discussionsupporting

confidence: 53%

Population Structure of Denison?s barb, Puntius denisonii (Pisces:Cyprinidae): A Species Complex Endemic to the Western Ghats of India

John¹,

Peter²,

Gopalakrishnan³

2013

J Phylogen Evolution Biol

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Genetic and morphologic variation, haplotype relationships, and structuring of populations within Puntius denisonii and its close related species Puntius chalakkudiensis have been tested using molecular and biometric data, to infer phylogeographic patterns. Sequences of mitochondrial DNA ATPase 8 and 6 genes, and morphometric data, were used to find population structuring. Specimens were collected from 7 locations in the southern region of Western Ghats, a global biodiversity hotspot in India. Biometric analysis revealed apparent heterogeneity in the morphology and color pattern between the species at juvenile and adult stages, and among different geographically separated populations of these species. High values for mean pair wise distances and a high proportion of the total variance attributed to differences between the geographically isolated populations with AMOVA, indicated clear population structuring within these species. Extremely high values for Pair wise F ST and significantly lower Nm values observed among the populations studied, suggested little or no effective gene flow among them. Constructed phylogenies further confirmed a high degree of population structuring within the species, showing local endemism with population specific haplotypes forming a species complex. The present study thus estimates the validity of subpopulations within P. denisonii and P. chalakkudiensis; clarifies the relationships of populations of P. denisonii with that of P. chalakkudiensis, and also indicates the presence of four different independent evolutionary lineages forming cryptic species within P. denisonii. The study further emphasizes the need for a conservation policy to be developed for each population of both species, separately based on MUs (Management Units).

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Section: Discussionsupporting

confidence: 53%

Population Structure of Denison?s barb, Puntius denisonii (Pisces:Cyprinidae): A Species Complex Endemic to the Western Ghats of India

John¹,

Peter²,

Gopalakrishnan³

2013

J Phylogen Evolution Biol

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“…DNA was extracted from one hind leg of each parasitoid, which meant the specimen could be used for morphological analyses and it reduced the chances of inadvertently amplifying the DNA of parasitoid-associated Wolbachia (Linares et al, 2009). DNA was extracted using the DNeasy Blood & Tissue Kit (QIAGEN) according to the manufacturer's protocol and resuspended in 100 µl of AE buffer.…”

Section: Dna Extraction and Sequencingmentioning

confidence: 99%

The parasitoid species complex associated with sexual and parthenogenetic Naryciinae (Lepidoptera: Psychidae): Integrating ecological and molecular analyses

Elzinga¹,

Zwakhals²,

Mappes³

et al. 2011

Eur. J. Entomol.

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Abstract. This study describes the parasitoid species complex associated with seven closely related species of sexual (Siederia rupicollella, S. listerella, Dahlica lazuri, D. charlottae and D. lichenella) and parthenogenetic (Dahlica fennicella and D. triquetrella) Naryciinae (Lepidoptera: Psychidae) in Central Finland. A thorough ecological analysis of all the species of parasitoids recorded was combined with analyses of molecular data. Mitochondrial and nuclear DNA data were obtained from all the species in order to (1) detect cryptic species associated with host specialization, (2) assign undescribed males to females, and (3) verify the morphological identification of closely related species. A DNA barcoding technique was employed to identify host species from parasitized larval remains. By sampling more than 10,000 host larvae, of which 25.7% were parasitized, nine parasitoid species were identified morphologically, including both koinobionts (Ichneumonidae: Diadegma incompletum, Macrus parvulus, Trachyarus borealis, T. solyanikovi, T. fuscipes, T. brevipennis and Braconidae: Meteorus affinis) and idiobionts (Ichneumonidae: Orthizema flavicorne, Gelis fuscicornis).Ecological characteristics such as time and mode of host attack, time of emergence and level of specialization differed widely. The results show that differences in parasitoid biology need to be taken into account when studying differences in percentage parasitism of sexual and parthenogenetic Naryciinae. The molecular data revealed that one parasitoid species M. parvulus may consist of two cryptic forms associated with the sexual and parthenogenetic hosts, respectively. The data further establishes that T. brevipennis and some T. fuscipes are in fact morphotypes of one species. The large variation in mitochondrial DNA within species and its inconsistency with nuclear DNA demonstrate that current species and genus delimitation is inadequate in the Trachyarus species group. Our study shows that it is essential to use DNA barcoding methods when investigating host-parasitoid complexes.

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“…The most commonly used genes for the differentiation of insect species have been ribosomal RNA (18S, 28S, internal transcribed spacers) and mitochondrial cytochrome c oxidase I (COI) (Simon et al, 1994;Li et al, 2005). In fact, sequence diversity in the 5' region of the COI gene has been established as the standard for species identification across the animal kingdom (Hebert et al, 2003;Miller, 2007;Linares et al, 2009). Several prior studies (Hardy et al, 2008;Rung et al, 2008;Ashfaq et al, 2010) have examined mealybug classification based on sequence variation in ribosomal and mitochondrial DNA.…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogenetic analysis of a scale insect (Drosicha mangiferae; Hemiptera: Monophlebidae) infesting mango orchards in Pakistan

Ashfaq¹,

Ara²,

Noor³

et al. 2011

Eur. J. Entomol.

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Abstract. Mango orchards in Pakistan are attacked by the scale insect, Drosicha mangiferae (Hemiptera: Monophlebidae), commonly called the "mango mealybug". This insect is univoltine, active from December through May and targets multiple host plants. We used DNA nucleotide sequences to characterize and determine the phylogenetic status of D. mangiferae. Mango mealybugs were collected from several tree species from different localities and patterns of phylogenetic and genetic diversity were examined at both nuclear (18S, ITS1) and mitochondrial (COI) genes. Phylogenetic analysis confirms that the mango mealybug belongs to the family Monophlebidae. Minor genetic differences in both the ITS1 and the COI barcode region were noted among D. mangiferae collected from different geographic localities. These genetic differences revealed the existence of two genotypes of D. mangiferae that are region specific but not host-specific.

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High mitochondrial diversity in geographically widespread butterflies of Madagascar: A test of the DNA barcoding approach

Cited by 79 publications

References 83 publications

Population Structure of Denison?s barb, Puntius denisonii (Pisces:Cyprinidae): A Species Complex Endemic to the Western Ghats of India

Population Structure of Denison?s barb, Puntius denisonii (Pisces:Cyprinidae): A Species Complex Endemic to the Western Ghats of India

The parasitoid species complex associated with sexual and parthenogenetic Naryciinae (Lepidoptera: Psychidae): Integrating ecological and molecular analyses

Molecular phylogenetic analysis of a scale insect (Drosicha mangiferae; Hemiptera: Monophlebidae) infesting mango orchards in Pakistan

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