High miR-324-5p expression predicts unfavorable prognosis of gastric cancer and facilitates tumor progression in tumor cells

Zheng, Zhong; Li, Jun; An, Junyan; Yu, Feng; Wang, Lirong

doi:10.1186/s13000-020-01063-2

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…MiR‐324‐5p has been reported to regulate cell proliferation and apoptosis under diverse conditions by targeting different mRNAs (Wang et al , 2015 ; Lin et al , 2018 ; Chen et al , 2019b ; Wan et al , 2020 ; Zheng et al , 2021 ). For example, miR‐324‐5p inhibits mitochondrial fusion and apoptosis in cardiomyocytes and endothelial progenitor cells by downregulating Mtfr1 (Wang et al , 2015 ; Chen et al , 2019b ; Huang et al , 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Interferon‐mediated repression of miR ‐324‐5p potentiates necroptosis to facilitate antiviral defense

Dou

et al. 2022

EMBO Reports

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Mixed lineage kinase domain‐like protein (MLKL) is the terminal effector of necroptosis, a form of regulated necrosis. Optimal activation of necroptosis, which eliminates infected cells, is critical for antiviral host defense. MicroRNAs (miRNAs) regulate the expression of genes involved in various biological and pathological processes. However, the roles of miRNAs in necroptosis‐associated host defense remain largely unknown. We screened a library of miRNAs and identified miR‐324‐5p as the most effective suppressor of necroptosis. MiR‐324‐5p downregulates human MLKL expression by specifically targeting the 3′UTR in a seed region‐independent manner. In response to interferons (IFNs), miR‐324‐5p is downregulated via the JAK/STAT signaling pathway, which removes the posttranscriptional suppression of MLKL mRNA and facilitates the activation of necroptosis. In influenza A virus (IAV)‐infected human primary macrophages, IFNs are induced, leading to the downregulation of miR‐324‐5p. MiR‐324‐5p overexpression attenuates IAV‐associated necroptosis and enhances viral replication, whereas deletion of miR‐324‐5p potentiates necroptosis and suppresses viral replication. Hence, miR‐324‐5p negatively regulates necroptosis by manipulating MLKL expression, and its downregulation by IFNs orchestrates optimal activation of necroptosis in host antiviral defense.

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Section: Discussionmentioning

confidence: 99%

Interferon‐mediated repression of miR ‐324‐5p potentiates necroptosis to facilitate antiviral defense

Dou

et al. 2022

EMBO Reports

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“…MiR-324-5p promotes the progression of thyroid papillary cancer via the PTPRD/CEBPD [13]. In addition, miR-324-5p reduced the tumor protein expression, phosphatase, and tensin homolog and may improve gastric cancer`s invasion and proliferation ability [14]. Furthermore, Wan et al [15] discovered that miR-324-5p promoted pancreatic cancer progression due to the tumor suppressor gene expression and kruppel-like factor 3.…”

Section: Discussionmentioning

confidence: 99%

“…MiR-324-5p has been shown to either inhibit or promote human cancer. For example, it is inhibitory for gallbladder [11] and colorectal cancer [12] but can show carcinogenic effects in thyroid papillary [13], pancreatic [14], and gastric cancer [15]. However, whether the expression levels of miR-324-5p can serve as a predictor for BC development remains to be revealed.…”

Section: Introductionmentioning

confidence: 99%

MiR-324-5P Functions as a Novel Serum Biomarker and Modulates Breast Cancer Malignant Activity

Zhang

et al. 2021

Preprint

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Introduction: The best management practices for patients with breast cancer（BC） involve early detection and deterioration prevention. Therefore, it is important to study the occurrence of BC and its development mechanism and explore more sensitive diagnostic and prognostic indicators.Objective: Our study explored the significance and the serum miR-324-5p application in the diagnosis and prognosis of BC and evaluated possible molecular mechanisms.Methods: MiR-324-5p expressions in the serum and tissue of patients with BC were observed by real-time fluorescence quantitative polymerase chain reaction (PCR). Next, the miR-324-5p diagnostic ability in BC was evaluated by the receiver operating characteristic curve. Kaplan-Meier analyzed the relationship between expression of miR-324-5P and prognosis. The Cox regression model evaluated the serum miR-324-5P prognostic value in predicting disease-free survival (DFS) and overall survival (OS). In functional studies, cell counting Kit-8 assay and Cloning experiment were employed to assess BC cells’ proliferation level. Transwell assays and wound healing assays were performed to determine the effect of miR-324-5p on BC cell invasion and migration. In addition, the flow cytometry test was conducted to determine the effect of miR-324-5p on the apoptotic rate of BC cells. Finally, bioinformatics tools were employed to detect the potential target genes of miR-324-5p in BC.Results: miR-324-5p expression is up-regulated in in BC cells, which is related to tumor stage（p=0.0291)and age( p=0.0278). In addition, a BC diagnosis based on serum miR-324-5p levels had an 66% sensitivity, 78% specificity, and the area under the curve was approximately 0.7581. DFS (p=0.027) and OS (p<0.017) in the low miR-324-5P expression group were higher than those in the high expression group. The tumor node metastasis staging、Ki-67 and miR-324-5P were independent prognostic factors for the survival of patients with BC. In addition, miR-324-5p had a positive effect on BC cell invasion and proliferation, thereby preventing apoptosis. Finally, bioinformatics synthesis showed that the miR-324-5p target genes in BC are cysteine-rich hydrophobic domain 1 (CHIC1) and kruppel-like factor 7 (KLF7).Conclusion: MiR-324-5p may perform its oncogenic functions by interacting with the CHIC1 and KLF7 genes, and its existence in serum could serve as a diagnostic and prognostic biomarker of BC.

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“…Mir-106a [24], Mir-21-5p [25], Mir-147 [26] and Mir-324-5p [27] inhibit proliferation and promote apoptosis of gastric cancer cells by directly targeting the tumor suppressor PTEN. Mir-21 [28], Mir-95-3p [29] and Mir-4295 [30] inhibit autophagy and regulate apoptosis-related proteins through PI3K/AK/MTOR signaling pathway.…”

Section: The Mechanism Of Mirnas and Gastric Cancermentioning

confidence: 99%

Research Progress on the Role of miRNAs in the Development of Gastric Cancer

Jiang¹,

Wang²

2023

IJCEMR

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Gastric cancer is one of the most common malignant tumors in the world, with poor prognosis and is the main cause of cancer-related death. The 5-year survival rate of early gastric cancer can reach 90%. However, due to the low diagnosis rate of early gastric cancer, most patients are diagnosed as advanced gastric cancer at the first visit. Therefore, in order to improve the survival rate of gastric cancer patients, early diagnosis is particularly important. At present, tumor serum markers such as carcinoembryonic antigen (CEA), antibodies to Helicobacter pylori, histopathology, endoscopy, and determination of digestiogen are the main methods for the diagnosis and evaluation of gastric cancer. However, the effectiveness and sensitivity of these methods in the diagnosis of early gastric cancer are low. Therefore, it is of great significance to find new biomarkers with higher sensitivity and specificity for early diagnosis of gastric cancer. MiRNAs are abnormally expressed in many cancers, and some miR-NAs are closely related to tumor development, progression and treatment. Therefore, miRNAs may be used as biomarkers for early diagnosis and prognosis of cancer in the future. In this review, the authors summarized the research progress of miRNAs in the development, early diagnosis and prognosis of gastric cancer, in order to provide new ideas for the future clinical treatment of gastric cancer.

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High miR-324-5p expression predicts unfavorable prognosis of gastric cancer and facilitates tumor progression in tumor cells

Cited by 7 publications

References 32 publications

Interferon‐mediated repression of miR ‐324‐5p potentiates necroptosis to facilitate antiviral defense

Interferon‐mediated repression of miR ‐324‐5p potentiates necroptosis to facilitate antiviral defense

MiR-324-5P Functions as a Novel Serum Biomarker and Modulates Breast Cancer Malignant Activity

Research Progress on the Role of miRNAs in the Development of Gastric Cancer

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