High lipid order of Arabidopsis cell‐plate membranes mediated by sterol and DYNAMIN‐RELATED PROTEIN1A function

Frescatada-Rosa, Márcia; Stanislas, Thomas; Backues, Steven K.; Reichardt, Ilka; Men, Shuzhen; Boutté, Yohann; Jürgens, Gerd; Moritz, Thomas; Bednarek, Sebastian Y.

doi:10.1111/tpj.12674

Cited by 31 publications

(17 citation statements)

References 50 publications

(149 reference statements)

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“…In this sense, it will be a major goal of future studies to determine the coincident properties of mixed membrane microdomains with regard to protein recruitment and binding. On the in vivo side, plant researchers have started using fluorescent probes for the detection of membrane lipid-ordered domains (Frescatada-Rosa et al, 2014;Gerbeau-Pissot et al, 2016), which might prove a powerful tool to link in vitro and in vivo studies. Considering the current status of sequence information and the range of available in vitro and in vivo methods, I am confident that the coming years will bring substantial advances in the field of plant PI signaling.…”

Section: Pi Functions In Plantsmentioning

confidence: 99%

Phosphoinositide signaling in plant development

2016

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The membranes of eukaryotic cells create hydrophobic barriers that control substance and information exchange between the inside and outside of cells and between cellular compartments. Besides their roles as membrane building blocks, some membrane lipids, such as phosphoinositides (PIs), also exert regulatory effects. Indeed, emerging evidence indicates that PIs play crucial roles in controlling polarity and growth in plants. Here, I highlight the key roles of PIs as important regulatory membrane lipids in plant development and function.

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Section: Pi Functions In Plantsmentioning

confidence: 99%

Phosphoinositide signaling in plant development

2016

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“…FRAP experiments were performed as described previously (Men et al, 2008;Boutté et al, 2010;Frescatada-Rosa et al, 2014). In brief, FRAP experiments were performed with a Zeiss LSM 780 confocal laser-scanning system mounted on a Zeiss Axio observer Z1 inverted microscope, employing a watercorrected C-Apochromat 403 objective, numerical aperture 1.2 (Zeiss).…”

Section: Frap Analysis Of Fluorescent Fusion Proteinsmentioning

confidence: 99%

A Framework for Lateral Membrane Trafficking and Polar Tethering of the PEN3 ATP-Binding Cassette Transporter

et al. 2016

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The outermost cell layer of plants, the epidermis, and its outer (lateral) membrane domain facing the environment are continuously challenged by biotic and abiotic stresses. Therefore, the epidermis and the outer membrane domain provide important selective and protective barriers. However, only a small number of specifically outer membrane-localized proteins are known. Similarly, molecular mechanisms underlying the trafficking and the polar placement of outer membrane domain proteins require further exploration. Here, we demonstrate that ACTIN7 (ACT7) mediates trafficking of the PENETRATION3 (PEN3) outer membrane protein from the trans-Golgi network (TGN) to the plasma membrane in the root epidermis of Arabidopsis (Arabidopsis thaliana) and that actin function contributes to PEN3 endocytic recycling. In contrast to such generic ACT7-dependent trafficking from the TGN, the EXOCYST84b (EXO84b) tethering factor mediates PEN3 outer-membrane polarity. Moreover, precise EXO84b placement at the outer membrane domain itself requires ACT7 function. Hence, our results uncover spatially and mechanistically distinct requirements for ACT7 function during outer lateral membrane cargo trafficking and polarity establishment. They further identify an exocyst tethering complex mediator of outer lateral membrane cargo polarity.

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“…More recently, it became obvious that membrane microdomains within a single cell are highly diverse and of different compositions (9). Generally, in the plant model, organisms' plasma membrane microdomains turned out to be important in plant defense (10,11), cell polarity (12,13), and general signaling properties of the plasma membrane (14,15).…”

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confidence: 99%

Cytoskeletal Components Define Protein Location to Membrane Microdomains*

Szymanski

Zauber

Erban

et al. 2015

Molecular & Cellular Proteomics

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The plasma membrane is an important compartment that undergoes dynamic changes in composition upon external or internal stimuli. The dynamic subcompartmentation of proteins in ordered low-density (DRM) and disordered high-density (DSM) membrane phases is hypothesized to require interactions with cytoskeletal components. Here, we systematically analyzed the effects of actin or tubulin disruption on the distribution of proteins between membrane density phases. We used a proteomic screen to identify candidate proteins with altered submembrane location, followed by biochemical or cell biological characterization in Arabidopsis thaliana. We found that several proteins, such as plasma membrane ATPases, receptor kinases, or remorins resulted in a differential distribution between membrane density phases upon cytoskeletal disruption. Moreover, in most cases, contrasting effects were observed: Disruption of actin filaments largely led to a redistribution of proteins from DRM to DSM membrane fractions while disruption of tubulins resulted in general depletion of proteins from the membranes. We conclude that actin filaments are necessary for dynamic movement of proteins between different membrane phases and that microtubules are not necessarily important for formation of microdomains as such, but rather they may control the protein amount present in the membrane phases. Molecular & Cellular Proteomics 14: 10.1074/ mcp.M114.046904, 2493-2509, 2015.Living cells need borders and molecular compartments for biochemical reactions and storage of metabolites. The plasma membrane therefore is a prerequisite for the evolution of different life forms. It consists of a phospholipid bilayer into which proteins and special lipid species such as sterols, sphingolipids, and glycolipids are inserted. The first complex model of plasma membrane was proposed in 1972 by Jonathan Singer and Garth Nicolson (1), replacing the concept of the plasma membrane as a strict protein-lipid-protein sandwich that was generally accepted until then. In Singer and Nicolson's model, the cell membrane is a two-dimensionally oriented viscous solution in which the membrane constituents are orientated in the most thermodynamically favorable manner, hiding hydrophobic hydrocarbon chains inside the lipid bilayer and exposing polar and ionic groups to the aqueous phase. This fluid mosaic model also implied that membrane proteins as well as lipid components are distributed in a homogeneous lipid bilayer at long range, but they can form specific aggregates and phases at short range, which were also termed "lipid rafts" or membrane microdomains.Over the past 30 years, it has become evident that the plasma membrane is not such a homogeneous structure as it was initially proposed. We now know that the lipid bilayer is asymmetric (2) and that the free diffusion of membrane proteins is restricted by their interactions with intracellular and extracellular components (3). More recently, Simons and Ikonen suggested that large ordered phases, enriched with cholesterol and sphin...

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High lipid order of Arabidopsis cell‐plate membranes mediated by sterol and DYNAMIN‐RELATED PROTEIN1A function

Cited by 31 publications

References 50 publications

Phosphoinositide signaling in plant development

Phosphoinositide signaling in plant development

A Framework for Lateral Membrane Trafficking and Polar Tethering of the PEN3 ATP-Binding Cassette Transporter

Cytoskeletal Components Define Protein Location to Membrane Microdomains*

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