High Lipid Induction in Microalgae for Biodiesel Production

Sharma, Kalpesh; Schuhmann, Holger; Schenk, Peer M.

doi:10.3390/en5051532

Cited by 753 publications

(432 citation statements)

References 105 publications

(129 reference statements)

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“…Lower nutrient concentrations also lead to a decline in algal growth, with limited photosynthesis when optimum amount of light is given. In order to continue photosynthesis at lower nutrient concentration, cells replenish their pool of electron carriers by shifting their metabolism from growth to fatty acid production for energy storage 15 . With this, increased uptake of nutrients by the cells also takes place for growth before shifting their metabolism to lipid production.…”

Section: Effect Of Phosphorus Concentrations On Nutrient Removal By Mmentioning

confidence: 99%

Growth, Lipid Productivity and Cellular Mechanism of Lipid Accumulation in Microalgae <i>Monoraphidium</i> sp. Following Different Phosphorous Concentrations for Biofuel Production

Dhup¹,

Kannan²,

Dhawan³

2017

Current Science

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Stress to an algal species can be caused by several factors ranging from temperature extremes to varying light intensities under which they accumulate lipids. Stress caused by nutrient limitation is known to induce maximum lipid accumulation in microalgae. In order to obtain an appropriate phosphate concentration exhibiting both high biomass and high lipid contents, four different concentrations of phosphate were implemented. Mechanism of lipid accumulation was also studied. Of the tested concentrations, no significant difference between growth rates was observed. Cultures with phosphate concentration 2.7 mg/l exhibited maximum increase in biomass productivity compared to control. But phosphate concentration 0.5 mg/l demonstrated the highest lipid productivity (8.75 mg/l/day), in accordance with lipid content (21.8%). Further, representation of TEM and fluorescent microscopic images depicted differences in lipid accumulation and sub-cellular ultrastructure at different concentrations of phosphate. Disappearance of phosphate and nitrate from the medium was also evaluated to study the efficient nutrient concentration and to better understand mechanism of lipid enhancement.

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Section: Effect Of Phosphorus Concentrations On Nutrient Removal By Mmentioning

confidence: 99%

Growth, Lipid Productivity and Cellular Mechanism of Lipid Accumulation in Microalgae <i>Monoraphidium</i> sp. Following Different Phosphorous Concentrations for Biofuel Production

Dhup¹,

Kannan²,

Dhawan³

2017

Current Science

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“…Microalgae are emerging as most sustainable candidate for biofuel production. Some advantages such as easily cultivable, usage of non-arable land, no competition with food crops for land and water, nutrients uptake from wastewater, ability to produce substantial amount of triacylglycerides (TAG) and shorter doubling time make them better than other available feedstocks (Kiran et al 2014a;Sharma et al 2012;Wang et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Influence of varying nitrogen levels on lipid accumulation in Chlorella sp.

Kiran

Pathak

Kumar

et al. 2016

Int. J. Environ. Sci. Technol.

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Microalgal lipids can be enhanced through varying nitrogen (N) content, and limited supply of nitrogen source seems to be valuable approach for increased lipid accumulation in microalgae. In this study, Chlorella sp. IM-02 was observed under fluorescence microscope for increased number of lipid bodies under nitrogen scarcity. Fourier transform infrared spectroscopy was used to determine spectral changes due to varying lipid content under nitrogen-starved (N 0 , without sodium nitrate), nitrogen-limited (N 0.1 , N 0.25 , N 0.5 and N 1.0 representing 0.1, 0.25, 0.5 and 1.0 g/L of sodium nitrate, respectively) and nitrogen-sufficient (N 1.5 , i.e., 1.5 g/L sodium nitrate) setting. Chlorophyll content was also monitored under these conditions as growth indicator. Various biochemical components viz. total carbohydrates, total proteins and total lipids were also estimated under varying nitrogen levels spectrophotometrically. On fourth day itself, maximum lipid productivity was observed in case of N 0.5 , which is having one-third of nitrogen concentration present in original growth media, BG-11. This concludes N 0.5 as suitable nitrogen provision for better production of lipids in Chlorella sp. IM-02 without much compromising the biomass production as both growth and lipid quantity are key parameters affecting the lipid productivity of any microalgal strain.

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“…Researchers have been trying to manipulate the algae biomass by various physiochemical parameters, genetic engineering, light sensitive photo-bioreactors etc. Various stress conditions have been studied with different experimental setups to obtain the desired lipid in high amount for biodiesel production (Sharma et al, 2012;Fan et al, 2014). Moreover an alga has the potential to survive and manipulate the lipid metabolic pathways under varying physiochemical conditions (Mohammed et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Effect of Salinity, pH, Light Intensity on Growth and Lipid Production of Microalgae for Bioenergy Application

Prakash¹,

Gautom²,

Sharma³

2015

OnLine Journal of Biological Sciences

100

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The crisis of energy producing molecules (fuels) is expected to increase in future, which is currently produced from crude mineral oil. Biodiesel is most reliable, non-toxic, biocompatible liquid fuel that can replace the existing unsustainable sources of energy. Among all the known sources, microalgae display high potential for the production of biodiesel owing to their numerous benefits like higher biomass productivities than plants, no agricultural land requirement, cultivation in waste water and accumulation of 20-50% triacylglycerols. Microalgae biomass and lipid content plays an important role in commercial production of biodiesel. The present work was carried out to develop an axenic culture of a potential microalga Chlorella sp. for high biomass and enhanced lipid accumulation. The important growth parameters like pH, light colour, light intensity and photoperiod were studied for better production of Chlorella biomass. The effect of salinity on cell growth was also studied and compared with normal Fogg's medium grown cells. The main biomolecules like carbohydrate, protein, lipid and chlorophyll content were also estimated with the help of standard biochemical methods in salt supplemented and without salt Fogg's medium. The cellular lipid content was increased by growing the cells under different salt concentrations. The micro algal strain showed highest growth of 0.822 g L −1 and 1.021g L −1 in Fogg's medium and under 0.2 M NaCl supplemented medium respectively. However, the maximum lipid production of 0.1842 g L −1 was estimated by growing the cells in Fogg's medium including 0.5 M NaCl with slight compromise in cell growth (0.858 g L −1 ). The lipid content of Chlorella sp. was found to be 26.84% as compared to 14% obtained under normal culture condition. Thus, growing Chlorella sp. under salt supplemented medium and optimizing light requirement will produce high biomass and oil for biodiesel production.

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High Lipid Induction in Microalgae for Biodiesel Production

Cited by 753 publications

References 105 publications

Growth, Lipid Productivity and Cellular Mechanism of Lipid Accumulation in Microalgae <i>Monoraphidium</i> sp. Following Different Phosphorous Concentrations for Biofuel Production

Growth, Lipid Productivity and Cellular Mechanism of Lipid Accumulation in Microalgae <i>Monoraphidium</i> sp. Following Different Phosphorous Concentrations for Biofuel Production

Influence of varying nitrogen levels on lipid accumulation in Chlorella sp.

Effect of Salinity, pH, Light Intensity on Growth and Lipid Production of Microalgae for Bioenergy Application

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