High lipid content and productivity of microalgae cultivating under elevated carbon dioxide

Abstract

This study examined the cell growth rate, lipid contents, lipid productivity, chlorophyll a concentration, and carbon dioxide tolerance of Chlorella vulgaris under various cultivation conditions. The pH, concentration of carbon dioxide in media, and light intensity variables were manipulated to obtain high lipid productivity. The optimum conditions were at pH 7.0, 2,930 lux, and 30 % carbon dioxide. Biomass concentration reached 1,288, 1,130, and 1,083 mg L -1 at 15, 30, and 50 % CO 2 after 6 days, respectively, implying that this strain has appreciable tolerance to carbon dioxide. The highest concentration of chlorophyll a occurred at 2,930 lux and decreased with increasing light intensity gradually. The maximum specific growth rate was 3.25 day -1 based on the dry weight and 4.63 day -1 based on the cell number. The lipid content (45.68 %) and lipid productivity (86.03 mg day -1 L -1 ) obtained in this study are higher than reported values in literatures. Hence, C. vulgaris is a good candidate for subsequent research in biodiesel production under elevated carbon dioxide concentration by microalgae.

Photosynthetic acclimation of Nannochloropsis oculata investigated by multi-wavelength chlorophyll fluorescence analysis

“…grown in wastewaters (Komolafe et al, 2014), the green algae Botryococcus sp. and Chlorella vulgaris (Huang et al, 2013(Huang et al, , 2014 and the eustigmatophycean Nannochloropsis oculata (Sukenik et al, 1989). The effect of various environmental factors, such as pH, concentration of carbon dioxide in the medium, and irradiance were shown to impact on lipid production of microalgae (Huang et al, 2014).…”

“…and Chlorella vulgaris (Huang et al, 2013(Huang et al, , 2014 and the eustigmatophycean Nannochloropsis oculata (Sukenik et al, 1989). The effect of various environmental factors, such as pH, concentration of carbon dioxide in the medium, and irradiance were shown to impact on lipid production of microalgae (Huang et al, 2014). Beyond these environmental parameters, the application of nanomaterials (such as CaO and MgO nanoparticles) has been found to be effective catalysts of lipid synthesis and lipid extraction procedures (Zhang et al, 2013).…”

Potential of microbial‐derived nutrients for aquaculture development

“…Moreover, it has been reported that the nutritional content of bacteria and microalgae can be significantly modified by applying specific culture media and growth conditions (Loo et al . ; Huang & Su ).The cell walls of yeast comprise from 25 to 32% of the cell weight (Ferreira et al . ).…”

Functionalized Fe3O4@Silica Core–Shell Nanoparticles as Microalgae Harvester and Catalyst for Biodiesel Production

“…A clear solution was obtained after stirring for another 30 min. [17] After centrifugation and washing with deionized water three times, we added various amounts of deionized water to the microalgae to prepare an algae/water mixture (i.e., algae concentrate). Finally, Fe 3 O 4 nanoparticles were collected using a magnetic stone, washed with water and methanol repeatedly to remove excess ammonia, and stored in absolute ethanol to prevent oxidation of samples.…”