High Light Acclimation in Green Microalgae

Finazzi, Guido; Minagawa, Jun

doi:10.1007/978-94-017-9032-1_21

Cited by 13 publications

(9 citation statements)

References 149 publications

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“…Lower Y 0 may also reveal differences in fast photoprotection strategy. State transitions play an important role in lowering excitation pressure on PSII in green microalgae (Finazzi and Minagawa ), which is contrary to, for instance, diatoms. In addition, alternative electron flows appear to be of significant importance in rerouting excess energy in both Chlorophyceae ( Chlamydomonas reinhardtii ) and Prasinophyceae ( Ostreococcus sp.…”

Section: Resultsmentioning

confidence: 99%

“…, Goss and Lepetit , Magdaong and Blankenship , Pinnola and Bassi ). In microalgae, its peculiarities have been extensively studied in green representatives (Finazzi and Minagawa , Erickson et al. ), in the Heterokont diatoms (Lepetit et al.…”

Section: What Is the Xanthophyll Cycle (Xc)‐related Nonphotochemical mentioning

confidence: 99%

“…), green algae (Niyogi et al. , Finazzi and Minagawa ), diatoms (Olaizola et al. , Lavaud and Goss ), and brown algae (Harker et al.…”

Section: What Is the Xanthophyll Cycle (Xc)‐related Nonphotochemical mentioning

confidence: 99%

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Diversity in Xanthophyll Cycle Pigments Content and Related Nonphotochemical Quenching (NPQ) Among Microalgae: Implications for Growth Strategy and Ecology

Lacour

Babin

Lavaud

2020

Journal of Phycology

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Xanthophyll cycle‐related nonphotochemical quenching (NPQ), which is present in most photoautotrophs, allows dissipation of excess light energy. Xanthophyll cycle‐related NPQ depends principally on xanthophyll cycle pigments composition and their effective involvement in NPQ. Xanthophyll cycle‐related NPQ is tightly controlled by environmental conditions in a species‐/strain‐specific manner. These features are especially relevant in microalgae living in a complex and highly variable environment. The goal of this study was to perform a comparative assessment of NPQ ecophysiologies across microalgal taxa in order to underline the specific involvement of NPQ in growth adaptations and strategies. We used both published results and data acquired in our laboratory to understand the relationships between growth conditions (irradiance, temperature, and nutrient availability), xanthophyll cycle composition, and xanthophyll cycle pigments quenching efficiency in microalgae from various taxa. We found that in diadinoxanthin‐containing species, the xanthophyll cycle pigment pool is controlled by energy pressure in all species. At any given energy pressure, however, the diatoxanthin content is higher in diatoms than in other diadinoxanthin‐containing species. XC pigments quenching efficiency is species‐specific and decreases with acclimation to higher irradiances. We found a clear link between the natural light environment of species/ecotypes and quenching efficiency amplitude. The presence of diatoxanthin or zeaxanthin at steady state in all species examined at moderate and high irradiances suggests that cells maintain a light‐harvesting capacity in excess to cope with potential decrease in light intensity.

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Section: Resultsmentioning

confidence: 99%

Section: What Is the Xanthophyll Cycle (Xc)‐related Nonphotochemical mentioning

confidence: 99%

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Diversity in Xanthophyll Cycle Pigments Content and Related Nonphotochemical Quenching (NPQ) Among Microalgae: Implications for Growth Strategy and Ecology

Lacour

Babin

Lavaud

2020

Journal of Phycology

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“…It is not surprising that light is the crucial factor regulating LHCSR3 level (Bonente et al ). It was shown that C. reinhardtii grown under low light is not able to effectively induce NPQ, while cultivation under high light makes this microalga capable of efficient NPQ development (Peers et al , Bonente et al , Finazzi and Minagawa , Quaas et al ). In the experiment carried out for the sake of the present paper, the dependence of the NPQ amplitude on light intensity in which C. reinhardtii was cultured was also observed (Fig.…”

Section: Resultsmentioning

confidence: 99%

Practical aspects of the measurements of non‐photochemical chlorophyll fluorescence quenching in green microalgae Chlamydomonas reinhardtii using Open FluorCam

Nowicka

2019

Physiologia Plantarum

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Methods of chlorophyll fluorescence measurements are widely used in the research on photosynthesis and ecophysiology of plants and algae. Among them, a very popular technique is pulse‐amplitude‐modulation (PAM) flourometry, which is simple to carry out, fast and non‐invasive. However, this method is also prone to generate artifacts if the experiments were not planned and executed properly. Application of this technique to algae brings additional complications, which need to be taken into consideration. Some of them are connected with sample preparation and setting of the protocols used, while another origin from the differences in the photosynthetic apparatus and regulation of photosynthesis in various algal groups when compared to vascular plants. In the present paper, some important practical aspects concerning PAM fluorometry measurements in the green microalga Chlamydomonas reinhardtii have been described, including the equipment settings and sample preparation. The impact of growth conditions, such as light, temperature and medium type on the induction of non‐photochemical quenching of chlorophyll fluorescence have been also tackled, as well as the question of state transitions occurring in darkness.

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“…Acidification of the lumen in turn activates another important photoprotective process, qE, which dissipates excess light energy (Horton et al ., ; Müller et al ., ; Niyogi and Truong, ). In green algae, LHCSR3 (Peers et al ., ) and PsbS (Correa‐Galvis et al ., ) are the major qE effectors mediating energy dissipation under environmental stresses like HL and also nutrient starvation (Finazzi and Minagawa, ). Indeed, NPQ induction is delayed in the flv mutants during HL phases (Figure S3c), even though the flv mutants accumulate LHCSR3 at similar levels to wt cc‐4533 (Figure b).…”

Section: Discussionmentioning

confidence: 99%

Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light

et al. 2018

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SUMMARYPhotosynthetic organisms have evolved numerous photoprotective mechanisms and alternative electron sinks/pathways to fine-tune the photosynthetic apparatus under dynamic environmental conditions, such as varying carbon supply or fluctuations in light intensity. In cyanobacteria flavodiiron proteins (FDPs) protect the photosynthetic apparatus from photodamage under fluctuating light (FL). In Arabidopsis thaliana, which does not possess FDPs, the PGR5-related pathway enables FL photoprotection. The direct comparison of the pgr5, pgrl1 and flv knockout mutants of Chlamydomonas reinhardtii grown under ambient air demonstrates that all three proteins contribute to the survival of cells under FL, but to varying extents. The FDPs are crucial in providing a rapid electron sink, with flv mutant lines unable to survive even mild FL conditions. In contrast, the PGRL1 and PGR5-related pathways operate over relatively slower and longer timescales. Whilst deletion of PGR5 inhibits growth under mild FL, the pgrl1 mutant line is only impacted under severe FL conditions. This suggests distinct roles, yet a close relationship, between the function of PGR5, PGRL1 and FDP proteins in photoprotection.

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High Light Acclimation in Green Microalgae

Cited by 13 publications

References 149 publications

Diversity in Xanthophyll Cycle Pigments Content and Related Nonphotochemical Quenching (NPQ) Among Microalgae: Implications for Growth Strategy and Ecology

Diversity in Xanthophyll Cycle Pigments Content and Related Nonphotochemical Quenching (NPQ) Among Microalgae: Implications for Growth Strategy and Ecology

Practical aspects of the measurements of non‐photochemical chlorophyll fluorescence quenching in green microalgae Chlamydomonas reinhardtii using Open FluorCam

Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light

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