High levels of human chymase expression in the pineal and pituitary glands

Baltatu, Ovidiu Constantin; Nishimura, Hikaru; Hoffmann, Sigrid; Stoltenburg, G.; Hăulică, I; Lippoldt, Andrea; Ganten, Detlev; Urata, Hidenori

doi:10.1016/s0006-8993(96)01474-6

Cited by 45 publications

(43 citation statements)

References 36 publications

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“…We al so sho wed mo de rate deg re e of im munos ta i ning in the lung sli des stai ned with MCC. This fin ding was si mi lar to tho se of Ura ta et al and Bal ta u et al 40,41 The che mo ki ne TARC is a li gand for the chemo ki ne re cep tor CCR4 ex pres sed on (Th)2-type CD4 T cells. Al ler gic air way inf lam ma ti on is charac te ri zed by a lo cal in cre a se in cells sec re ting Th2-type cyto ki nes.…”

Section: Discussionsupporting

confidence: 64%

Immunohistochemical Analysis of MCC, TARC and CD 104 Antigens in Human Lung Tissue

Korkmaz¹,

Akkuş²,

Yıldırım³

et al. 2010

Turkiye Klinikleri J Med Sci

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A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : The aim of this study was to in ves ti ga te the the dis tri bu ti on of CMA1 pro te in (MCC), CCL17 pro te in (TARC) and In teg rin be ta 4 (CD 104) mo noc lo nal an ti bo di es in nor mal adult hu man lung tissu e. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : In this study we exa mi ned the lung bi opsy spe ci mens ob ta i ned from the adult pati ents who un der went ope ra ti ons for dif fe rent re a sons in the De part ment of Lung Sur gery at Dic le Uni ver sity Hos pi tal, Di yar ba kır, Tur key. The tis su e samp les we re im me di a tely fro zen in li qu id nit ro gen at -196º C. The samp les we re im munos ta i ned by in di rect im mu ne pe ro xi da se tech ni qu e. As pri mary an ti bo di es, MCC, TARC and CD104 we re used. As se con dary an ti bo di es, 1:200 rab bit an ti -mo u se IgG pe ro xi da se di lu ted in PBS/BSA and 1:100 nor mal hu man se rum so lu ti on we re used. Ne ga ti ve con trol sta i ning was per for med using ir re levant mo u se mo noc lo nal an ti bo di es omit ting the pri mary an ti body step. Sec ti ons we re exa mi ned and pho tograp hed by Oly mpus BH2 light mic ros co pe. R Re e s su ul lt ts s: : We ob ser ved mo de ra te re ac ti on with vas cu lar en dot he li al cells and with vas cu lar smo oth musc le cells with MCC which was ai med to show ove rall the pre sen ce of human lung mast cells. TARC, which was ai med to ex press the fol li cu lar den tri tic cells, mo de ra tely re ac ted with fol li cu lar den tri tic cells, bronc hi al smo oth musc le cells and vas cu lar en dot he li al cells. CD104, which is the in teg rin β4 su bu nit that plays an im por tant ro le in the ad he si on of epit he li um to ba se ment mem bra nes, mo de ra tely re ac ted with vas cu lar en dot he li al cells and ma inly re ac ted with the fol li cu lar den tri tic cells of bronchi. C Co on nc c l lu u s si i o on n: : In our study, it was de fi ned that the ex pres si ons of the se mo le cu les ha ve im por tant role in un ders tan ding the func ti on of hu man lung tis su e. We re ac hed the conc lu si on that our study, by presen ting the dif fe ren ce bet we en the nor mal lung tis su e and pat ho lo gic lung tis su e with ex pres si on the re la ted pro te ins im mu no his toc he mi cally may constitute preliminary findings for exc lu ding the di se a ses li ke in tersti si al lung di se a se or chro nic obs truc ti ve lung di se a se for the aim of. Our preliminary findings may shed light on future studies. K Ke ey y W Wo or rd ds s: : CMA1 pro te in, hu man; CCL17 pro te in, hu man; In teg rin be ta4 Ö ÖZ ZE ET T A Am ma aç ç: : Bu ça lış ma da CMA1 pro te in (MCC), CCL17 pro te in (TARC) ve In teg rin be ta 4 (CD 104) monok lo nal an ti kor la rı nın nor mal eriş kin in san ak ci ğer do ku sun da ki da ğı lı mı nın in ce len me si amaç lan mış tır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Bu ça lış ma da, Dic le Üni ver si te si Has ta ne si Gö ğüs Cer ra hi si Bö lü mü'n de fark lı se b...

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Section: Discussionsupporting

confidence: 64%

Immunohistochemical Analysis of MCC, TARC and CD 104 Antigens in Human Lung Tissue

Korkmaz¹,

Akkuş²,

Yıldırım³

et al. 2010

Turkiye Klinikleri J Med Sci

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“…Microinjections of ANG-(1-12) (1 mM) at different rostrocaudal levels of the ARCN elicited similar cardiovascular responses ( Table 2). Microinjections of ANG II (1 mM) into the ARCN elicited greater cardiovascular responses compared with the same concentration of ANG- (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) at the same levels ( Table 2). The onset of cardiovascular responses to microinjections of ANG-(1-12) (1 mM) into the ARCN (11.2 Ϯ 1.2 s) was significantly (P Ͻ 0.05) longer than the onset of action (8.3 Ϯ 0.6 s) of microinjection of ANG II (1 mM) at the same site.…”

Section: Resultsmentioning

confidence: 97%

“…Cardiovascular responses to microinjections of ANG- (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) into the ARCN. The ARCN (ϳ2 mm long in the rostrocaudal direction) was arbitrarily divided into the following three equal segments (the coordinates mentioned for each segment indicate the level caudal to the bregma): the rostral segment (2.1-2.9 mm), middle segment (2.9 -3.7 mm), and caudal segment (3.7-4.5 mm).…”

Section: Resultsmentioning

confidence: 99%

“…Site specificity of ANG- (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)-induced responses in the ARCN. We (31) have previously reported that microinjections of NMDA (10 mM) into the ARCN at different rostrocaudal levels (2.3-4.16 mm caudal to the bregma) and the dorsomedial hypothalamic nucleus (DMN; 3.6 caudal to bregma, 0.2 lateral to the midline, and 9.0 deep from the dura) elicited pressor and tachycardic responses.…”

Section: Resultsmentioning

confidence: 99%

“…Bilateral microinjections of ANG- (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) into the NTS have been reported to elicit transient depressor responses and an attenuation of baroreflex sensitivity (1). Chronic immunoneutralization of endogenous brain ANG- (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) by intracerebroventricular injections of anti-ANG-(1-12) IgG in hypertensive rats have been reported to elicit antihypertensive effects (21). Several components of the renin-ANG system (RAS) have been identified in the ARCN.…”

mentioning

confidence: 99%

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The hypothalamic arcuate nucleus: a new site of cardiovascular action of angiotensin-(1–12) and angiotensin II

Arakawa

Chitravanshi

Sapru

2011

American Journal of Physiology-Heart and Circulatory Physiology

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Arakawa H, Chitravanshi VC, Sapru HN. The hypothalamic arcuate nucleus: a new site of cardiovascular action of angiotensin-(1-12) and angiotensin II. Am J Physiol Heart Circ Physiol 300: H951-H960, 2011. First published December 24, 2010 doi:10.1152/ajpheart.01144.2010.-The hypothalamic arcuate nucleus (ARCN) has been reported to play a significant role in cardiovascular regulation. It has been hypothesized that the ARCN may be one of the sites of cardiovascular actions of angiotensins (ANGs). Experiments were carried out in urethaneanesthetized, artificially ventilated, adult male Wistar rats. The ARCN was identified by microinjections of N-methyl-D-aspartic acid (NMDA; 10 mM). Microinjections (50 nl) of ANG-(1-12) (1 mM) into the ARCN elicited increases in mean arterial pressure (MAP), heart rate (HR), and greater splanchnic nerve activity (GSNA). The tachycardic responses to ANG-(1-12) were attenuated by bilateral vagotomy. The cardiovascular responses elicited by ANG-(1-12) were attenuated by microinjections of ANG II type 1 receptor (AT1R) antagonists but not ANG type 2 receptor (AT2R) antagonist. Combined inhibition of ANG-converting enzyme (ACE) and chymase in the ARCN abolished ANG-(1-12)-induced responses. Microinjections of ANG II (1 mM) into the ARCN also increased MAP and HR. Inhibition of ARCN by microinjections of muscimol (1 mM) attenuated the pressor and tachycardic responses to intravenously administered ANG-(1-12) and ANG II (300 pmol/kg each). These results indicated that 1) microinjections of ANG-(1-12) into the ARCN elicited increases in MAP, HR, and GSNA; 2) HR responses were mediated via both sympathetic and vagus nerves; 3) AT1Rs, but not AT2Rs, in the ARCN mediated ANG-(1-12)-induced responses; 4) both ACE and chymase were needed to convert ANG-(1-12) to ANG II in the ARCN; and 5) ARCN plays a role in mediating the cardiovascular responses to circulating ANGs. blood pressure; heart rate; microinjection; N-methyl-D-aspartic acid; sympathetic nerve activity THE HYPOTHALAMIC ARCUATE NUCLEUS (ARCN) may play a significant role in cardiovascular regulation (10, 38). Consistent with this notion, we (31) have recently reported that chemical stimulation of the ARCN elicited increases in mean arterial pressure (MAP), heart rate (HR), and sympathetic nerve activity (SNA). These reports have provided a basis for investigations on different neurotransmitters and neuromodulators in the ARCN that may play a role in the regulation of cardiovascular function in normal and pathological states.A new endogenous angiotensin (ANG), ANG-(1-12), has recently been identified (30,44). Intravenous administration of this peptide has been reported to elicit an immediate pressor response in the rat, and this effect was blocked by prior administration of an ANG-converting enzyme (ACE) inhibitor or an ANG II type 1 receptor (AT 1 R) antagonist (30). These data indicated that in the periphery, ANG-(1-12) exerts its actions via a rapid conversion to ANG II (30). High concentrations of ANG-(1-12) have been reported in th...

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The detection of mast cell subpopulations in formalin-fixed human tissues using a new monoclonal antibody specific for chymase

1999

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Chymase is an important marker for human mast cells as well as a mediator of inflammation and matrix remodelling, but research into chymase‐containing mast cell subpopulations has been hampered by the lack of reagents suitable for use with formalin‐fixed tissue. A monoclonal antibody to chymase (designated CC1) was prepared by immunizing a mouse with chymase purified from human skin, fusing the splenocytes with NS‐1 myeloma cells, and screening the hybridoma supernatants by ELISA with recombinant human prochymase isolated from a baculovirus expression system. This antibody bound to chymase in western blots and bound selectively to cells with the morphology and distribution of mast cells in paraffin wax sections of skin, synovium, lung, and heart. In sequential sections and with double‐labelling experiments, chymase was localized to cells which contained mast cell tryptase; in contrast to previous reports, no evidence was found for its presence in endothelial cells or any other cell type. The antibody permitted chymase‐containing mast cells to be detected in formalin‐fixed tissues, and the numbers identified were similar to those in tissues fixed with Carnoy's or ethanol fixatives. Immunocytochemistry with antibody CC1 provides for the first time a sensitive and specific means for the detection of chymase in routinely fixed tissues and should prove valuable in studying mast cell subsets in disease. Copyright © 1999 John Wiley & Sons, Ltd.

show abstract

High levels of human chymase expression in the pineal and pituitary glands

Cited by 45 publications

References 36 publications

Immunohistochemical Analysis of MCC, TARC and CD 104 Antigens in Human Lung Tissue

Immunohistochemical Analysis of MCC, TARC and CD 104 Antigens in Human Lung Tissue

The hypothalamic arcuate nucleus: a new site of cardiovascular action of angiotensin-(1–12) and angiotensin II

The detection of mast cell subpopulations in formalin-fixed human tissues using a new monoclonal antibody specific for chymase

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