High levels of circulating prostaglandin F2α associated with ovulation stimulate female sexual receptivity and spawning behavior in the goldfish (Carassius auratus)

Sorensen, Peter W.; Appelt, Christopher W.; Stacey, N. E.; Goetz, Fredrick Wm.; Brash, Alan R.

doi:10.1016/j.ygcen.2018.06.014

Cited by 27 publications

(16 citation statements)

References 37 publications

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“…Additionally, masculinized fish were attracted to a mixture of the three PGFs, which we also showed to be released by conspecifics treated with PGF2α following established techniques known to mimic natural pheromone release in Eurasian Carps [11,12]. Very likely, this sex pheromone is normally released in the urine by ovulated, sexually receptive Silver Carp females, as is the case for the Goldfish and Common Carp [27]; PGF2α is intimately tied to the process of ovulation and spawning behavior in the Goldfish and presumably its relatives [5,14]. As with the Common Carp, the Silver Carp pheromone signal appears to be…”

Section: Discussionmentioning

confidence: 63%

“…It seems especially notable that PGF 2α -implanted Silver Carps could be used an inexpensive platform to produce and release pheromones in wild fishes, as has been shown in the invasive Common Carp [12]. Additionally, because hormonal PGF 2α is known to function as a female reproductive hormone that drives female behavior [14], it creates the opportunity to use PGF 2α -implanted carps as Judas fish, which can lead managers to shoals of mature fish [34,35]. Lastly, recent studies have shown that PGF can be measured in waterways, making it possible to track PGFs together with eDNA to survey for Bigheaded Carps, while also identifying Carp gender and health [9,35].…”

Section: Discussionmentioning

confidence: 99%

“…Olfactory sensitivity in Silver Carp was discerned using electro-olfactogram recording (EOG), an extracellular recording technique that accurately assesses olfactory organ sensitivity following well established protocols [13,14,17,18]. Briefly, fish were anesthetized using 0.01% MS-222 (Western Chemical Inc., Ferndale, WA, USA), placed on a recording stand, which irrigated their gills with 0.001% MS-222, after which the skin flap protecting their nares was carefully removed, while a recording electrode was placed over their olfactory epithelium and an indifferent electrode was placed on their skin.…”

Section: Determining Olfactory Sensitivity Of Untreated and Masculinimentioning

confidence: 99%

“…Females of these seasonal scramble spawning Cyprinid carps [10] employ a mixture of several sex steroid hormones and their metabolites as a priming pheromone to synchronize egg and sperm maturity during the perio-ovulatory period, and then a mixture of prostaglandin F 2α (PGF 2α ) and its metabolites, 15keto-prostaglandin F 2α (15K-PGF 2α ) and 13,14dihydro-15keto-PGF 2α (dh15K-PGF 2α ) as a spawning (releasing) pheromone [6,10,12]. PGF 2α is known to be produced by ovulated Goldfish as a hormone to stimulate female sexual behavior before functioning as a sex pheromone that is released within minutes of ovulation and spawning [12][13][14]. The PGF pheromone both attracts males and signals that females are sexually receptive and carrying eggs, stimulating courtship.…”

Section: Introductionmentioning

confidence: 99%

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A Blend of F Prostaglandins Functions as an Attractive Sex Pheromone in Silver Carp

Sorensen

Rue

Leese

et al. 2019

Fishes

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A series of laboratory experiments tested the hypothesis that the Silver Carp (Hypophthalmichthys molitrix), an invasive river carp from China, employs a prostaglandin F2α-derived sex pheromone that is attractive and species-specific. Using electro-olfactogram recording (EOG), we found that the olfactory system of this species is acutely sensitive to three F-series prostaglandins (PGFs) at picomolar concentrations and that this sensitivity is enhanced when juveniles are masculinized using androgens, consistent with expectations of a sex pheromone. Individual PGFs had behavioral activity but it was low, suggesting a mixture might be important. To pursue this possibility, we implanted carps with osmotic pumps containing prostaglandin F2α (PGF2α), a treatment previously shown to elicit release of a PGF-based spawning pheromone in the Common Carp. We found that PGF2α-implanted Silver Carp released a species-specific odor that contained a blend of PGF2α and two of its metabolites, which masculinized individuals detected and were attracted to with high sensitivity. Finally, we found that a mixture of these PGFs was attractive to masculinized Silver Carp, while a different mixture released by Bighead Carp was not. We conclude that Silver Carp likely use a species-specific PGF2α-derived sex pheromone that is probably released at spawning and might be useful in its control. Confirmatory studies that explore pheromone function in naturally mature Silver Carp using natural odors in the field should now be conducted to further confirm our proof-of-concept study.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Determining Olfactory Sensitivity Of Untreated and Masculinimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Blend of F Prostaglandins Functions as an Attractive Sex Pheromone in Silver Carp

Sorensen

Rue

Leese

et al. 2019

Fishes

View full text Add to dashboard Cite

show abstract

“…In teleosts, plasma PGF 2a levels in successfully ovulated females are significantly higher than those in unsuccessfully ovulated females in small-scale pacu (Piaractus mesopotamicus), indicating the importance of PGF 2a in inducing ovulation (35). In goldfish (Carassius auratus), PGF 2a functions as a blood-borne behavior hormone that induces spawning behavior (36,37). These experiments suggested that PGF 2a has similar activity in inducing ovulation in some externally fertilizing fish.…”

Section: Introductionmentioning

confidence: 94%

Arg-Vasotocin Directly Activates Isotocin Receptors and Induces COX2 Expression in Ovoviviparous Guppies

Lyu

Wang

et al. 2021

Front. Endocrinol.

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Oxytocin (OT) is a crucial regulator of reproductive behaviors, including parturition in mammals. Arg-vasopressin (AVP) is a nonapeptide homologous to Arg-vasotocin (AVT) in teleosts that has comparable affinity for the OT receptor. In the present study, ovoviviparous guppies (Poecilia reticulata) were used to study the effect of AVT on delivery mediated by the activation of prostaglandin (PG) biosynthesis via isotocin (IT) receptors (ITRs). One copy each of it and avt and two copies of itrs were identified in guppies. The results of the affinity assay showed that various concentrations of AVT and IT (10−6, 10−7, and 10−8 mol/L) significantly activated itr1 (P < 0.05). In vitro experiments revealed significant upregulation (P < 0.05) of cyclooxygenase 2 (cox2), which is the rate-limiting enzyme involved in PG biosynthesis, and itr1 by AVT and IT. Furthermore, dual in situ hybridization detected positive signals for itr1 and cox2 at the same site, implying that ITR1 may regulate cox2 gene expression. Measurement of prostaglandin F2a (PGF2a) concentrations showed that AVT induced PGF2a synthesis (P < 0.05) and that the effect of IT was not significant. Finally, intraperitoneal administration of PGF2a significantly induced premature parturition of guppies. This study is the first to identify and characterize AVT and ITRs in guppies. The findings suggest that AVT promotes PG biosynthesis via ITR and that PGF2a induces delivery behavior in ovoviviparous guppies.

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Chemical cues for intraspecific chemical communication and interspecific interactions in aquatic environments: applications for fisheries and aquaculture

2021

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Aquatic organisms detect chemical cues to sense the local environment, for example, to find a mate, locate food, and identify danger. Knowledge of chemical cues can be used in aquaculture, in practical applications such as controlling mating behavior to increase fertility, enhance feeding, and decrease stress; in fisheries, by catching selected species with low-cost artificial attractants; and to address maritime issues, by decreasing biofouling. Aquatic organisms also detect chemical cues related to global environmental changes, ocean acidification, and increases in ocean plastics, all of which can affect their chemosensory behaviors. Here we discuss the nature of chemical cues and chemosensory biology and ecology of aquatic organisms, and potential applications with an emphasis on sex pheromones in commercially important and well-studied animals, namely, decapod crustaceans and fish.

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High levels of circulating prostaglandin F2α associated with ovulation stimulate female sexual receptivity and spawning behavior in the goldfish (Carassius auratus)

Cited by 27 publications

References 37 publications

A Blend of F Prostaglandins Functions as an Attractive Sex Pheromone in Silver Carp

A Blend of F Prostaglandins Functions as an Attractive Sex Pheromone in Silver Carp

Arg-Vasotocin Directly Activates Isotocin Receptors and Induces COX2 Expression in Ovoviviparous Guppies

Chemical cues for intraspecific chemical communication and interspecific interactions in aquatic environments: applications for fisheries and aquaculture

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