High levels of circulating endothelial microparticles in patients with acute coronary syndromes

Bernal‐Mizrachi, Leon; Jy, Wenche; Jiménez, Joaquín J.; Pastor, Juan Antonio; Mauro, Lucía Mercedes; Horstman, Lawrence L.; Marchena, Eduardo de; Ahn, Yeon S.

doi:10.1016/s0002-8703(03)00103-0

Cited by 401 publications

(322 citation statements)

References 40 publications

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“…Our results are in agreement with previous studies demonstrating elevated platelet and endothelial MPs in ACS (31)(32)(33). We have also now demonstrated that the levels of leukocyte (neutrophil and monocyte) MPs are elevated in ACS.…”

Section: Discussionsupporting

confidence: 93%

Microparticles in acute coronary syndrome

Mavroudis

Eleftheriou²,

Hong³

et al. 2017

Thrombosis Research

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Section: Discussionsupporting

confidence: 93%

Microparticles in acute coronary syndrome

Mavroudis

Eleftheriou²,

Hong³

et al. 2017

Thrombosis Research

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“…[8][9][10] Elevated numbers of circulating microparticles have been found in patients with a great variety of diseases with vascular involvement and hypercoagulability. [11][12][13][14][15] To the best of our knowledge, there are no reported studies that evaluated circulating microparticles in impotent diabetic men. Therefore, the aims of this study were (a) to evaluate circulating endothelial (EMP) and platelet microparticles (PMP) in diabetic men with ED, (b) to address the relation between circulating microparticles and the severity of ED and (c) to assess the relation between circulating microparticles and endothelial dysfunction.…”

Section: Introductionmentioning

confidence: 99%

Endothelial microparticles correlate with erectile dysfunction in diabetic men

et al. 2006

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Cell-derived microparticles are supposed to be involved in endothelial dysfunction and atherogenesis. This study aimed to evaluate circulating microparticles in diabetic subjects with erectile dysfunction (ED) and their relation with endothelial dysfunction. Thirty diabetic men with ED and 20 age-matched control subjects without ED were assessed for circulating microparticles and endothelial dysfunction. Flow cytometry was used to assess microparticles by quantification of circulating endothelial (EMP, CD31 þ /CD42b À ) and platelet (PMP, CD31 þ /CD42b þ ) microparticles in peripheral blood. Endothelium-dependent flow-mediated dilation (FMD) was evaluated in the right brachial artery after reactive hyperemia. Compared with non-diabetic subjects, diabetic men presented significantly higher numbers of EMP (P ¼ 0.001), and reduced FMD (P ¼ 0.01), with a significant inverse correlation between the number of circulating EMP and the International Index of Erectile Function (IIEF) score (r ¼ À0.457, P ¼ 0.01). Multivariate analysis correcting for age, anthropometric indices, glucose and lipid parameters, FMD and PMP identified EMP as the only independent predictor for IIEF score (P ¼ 0.03). EMP are elevated in impotent diabetic subjects and independently involved in the pathogenesis of ED.

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“…Endothelium-derived microparticles are more abundant in patients with ACS and appear to mirror the level of endothelial dysfunction (10). In addition, plaque stability correlates with the level of circulating microparticles where the expression of specific CD antigens is significantly increased in patients with high risk lesions (11).…”

mentioning

confidence: 99%

Using Antibody Arrays to Detect Microparticles from Acute Coronary Syndrome Patients Based on Cluster of Differentiation (CD) Antigen Expression

Lal

Brown

Nguyen

et al. 2009

Molecular & Cellular Proteomics

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Microparticles circulate in plasma and have recently emerged as potential inflammatory markers in cardiovascular disease. They are fragments of cell membranes that express cluster of differentiation (CD) antigens and are present at elevated levels in patients with acute coronary syndrome. We have developed a novel method for the rapid detection of microparticles in plasma using a fluorescence-based antibody array system. Isolated microparticles are captured on anti-CD antibody spots immobilized on a nitrocellulose membrane. These CD antibodies are directed against extracellular epitopes, whereas the intracellular exposed surface of the microparticles is labeled with a fluorescent anti-annexin antibody. The array is then scanned and quantified. A pilot study was undertaken to compare microparticle CD antigen expression in acute coronary syndrome and healthy subjects. Ten CD antigens (44, 45, 54, 62E, 79, 102, 117, 130, 138, and 154) had significantly increased expression in the disease group relative to the healthy controls. These results were then verified using flow cytometry and scanning electron microscopy. Although we have focused our analysis on changes in microparticle CD antigen expression, this technique is amenable to analyzing other surface markers. Microparticles can be derived from a wide variety of cell types, so selection of the primary antibody can be tailored to the cell origin that is to be investigated. Molecular & Cellular Proteomics 8: 799 -804, 2009.

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High levels of circulating endothelial microparticles in patients with acute coronary syndromes

Cited by 401 publications

References 40 publications

Microparticles in acute coronary syndrome

Microparticles in acute coronary syndrome

Endothelial microparticles correlate with erectile dysfunction in diabetic men

Using Antibody Arrays to Detect Microparticles from Acute Coronary Syndrome Patients Based on Cluster of Differentiation (CD) Antigen Expression

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