High levels of chromosomal differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae)

Moura, Rita de Cássia de; Melo, Natoniel Franklin de; Souza, Maria José de

doi:10.1590/s1415-47572008000300007

Cited by 9 publications

(10 citation statements)

References 25 publications

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“…However, numeric and morphologic variation in chromosomes of different taxa, for example, in families and subfamilies, have been described in the literature. Different structural rearrangements such as Robertsonian rearrangements and in tandem fusions and fissions can be responsible for such variations (Smith and Virkki, 1978;Vidal and Nocera, 1984;Petitpierre et al, 1991;Schneider et al, 2006;Moura et al, 2008;Cabral-de-Mello et al, 2008). The 2 species described herein differed with regard to the diploid number, chromosomal morphology, and sex chromosome bivalent.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetics of the darkling beetles Zophobas aff. confusus and Nyctobates gigas (Coleoptera, Tenebrionidae)

Lira-Neto

Silva²,

Moura³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Males of Zophobas aff. confusus and Nyctobates gigas (Tenebrionidae) collected in the State of Pernambuco, Brazil, were studied through conventional staining, C-banding, silver nitrate impregnation (AgNO 3 ), and the base specific fluorochromes CMA 3 and DAPI. Z. aff. confusus was found to have 2n = 20 (9+Xyp) while N. gigas exhibited 2n = 18 (8+neoXY). Large pericentromeric blocks of constitutive heterochromatin (CH) were detected throughout the autosomal complement of the two species, except in one autosomal pair of N. gigas in which no heterochromatic block was observed. The sex chromosomes of both species were almost totally heterochromatic. Double staining with CMA 3 /DA (distamycin) and DAPI/DA marked CH in Z. aff. confusus. However, DAPI staining was more intense. N. gigas was found to possess blocks of CH-positive CMA 3 and homogeneous DAPI. AgNO 3 staining also revealed differences between the two species. In Z. confusus an NOR was observed in the sexual bivalent Xyp and N. gigas was found to have an autosomal NOR.

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Section: Discussionmentioning

confidence: 99%

Cytogenetics of the darkling beetles Zophobas aff. confusus and Nyctobates gigas (Coleoptera, Tenebrionidae)

Lira-Neto

Silva²,

Moura³

et al. 2012

Genet. Mol. Res.

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“…The presence of B chromosomes in Coleoptera has been reported in B80 species, but these studies were focused on the presence or absence of this element, with no description of frequency in populations or in relation to its molecular content (Camacho, 2005;Angus et al, 2007;Moura et al, 2008). In the family Scarabaeidae, the presence of B chromosomes was described in representatives of the Cetoniinae and Scarabaeinae subfamilies .…”

Section: The B Chromosomementioning

confidence: 99%

Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

2009

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Chromosomal banding techniques and repetitive DNA mapping are useful tools in comparative analysis and in the elucidation of genome organization of several groups of eukaryotes. In this study, we contributed to the knowledge of Coleoptera genomes by reporting the chromosomal organization of repetitive DNA sequences, as well as the presence and characteristics of a B chromosome in two natural populations of Dichotomius geminatus (Coleoptera; Scarabaeidae) using classical, chromosomal banding and molecular cytogenetic techniques. As in other coleopteran species, the heterochromatin was mainly concentrated in pericentromeric regions and the B chromosome was composed almost entirely of heterochromatin. Physical mapping using double fluorescent in situ hybridization was performed for the first time in Coleoptera; using DNA probes for 5S and 18S ribosomal RNA (rRNA) and histone H3 genes, we showed that ribosomal 18S rDNAs are located in chromosomes 3 and 4, whereas 5S rRNA and histone H3 genes are colocalized in chromosomal pair 2 and show an apparently interspersed organization. Moreover, these genes are not present in the B chromosome, suggesting that the B chromosome did not originate from chromosomal pairs 2, 3 or 4. On the other hand, mapping of the C 0 t-1 DNA fraction showed that the B chromosome is enriched in repetitive DNA elements, also present in the standard complement, indicating an intraspecific origin of this element in D. geminatus. These results will contribute to our understanding of genome organization and evolution of repetitive elements in Coleoptera and other insects regarding both A and B chromosomes.

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“…Among Coleoptera species, the studies reporting the presence of B chromosomes have generally focused on the presence or absence of this element and have not considered their frequency in the population or their molecular content [18,21,23,36]. The presence of B chromosomes was reported in representatives of the Cetoniinae and Scarabaeinae, subfamilies of Scarabaeidae [21,22].…”

Section: Discussionmentioning

confidence: 99%

“…In Coleoptera, the presence of B chromosomes has been described in approximately 80 species belonging to several families, including Buprestidae [18], Cantharidae [19], Cicindelidae [20] and Scarabaeidae [21,22]. In general, the studies in Coleoptera have concentrated on the presence or absence of B chromosomes in species, with few reports covering their frequency in populations and/or their molecular content [18,21-23].…”

Section: Introductionmentioning

confidence: 99%

“…In general, the studies in Coleoptera have concentrated on the presence or absence of B chromosomes in species, with few reports covering their frequency in populations and/or their molecular content [18,21-23]. There are a few reports on the presence of B chromosomes in the Scarabaeidae family, including species of the Scarabaeinae and Cetoniinae subfamilies [21,22].…”

Section: Introductionmentioning

confidence: 99%

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B chromosome in the beetle Coprophanaeus cyanescens (Scarabaeidae): emphasis in the organization of repetitive DNA sequences

2012

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BackgroundTo contribute to the knowledge of coleopteran cytogenetics, especially with respect to the genomic content of B chromosomes, we analyzed the composition and organization of repetitive DNA sequences in the Coprophanaeus cyanescens karyotype. We used conventional staining and the application of fluorescence in situ hybridization (FISH) mapping using as probes C0t-1 DNA fraction, the 18S and 5S rRNA genes, and the LOA-like non-LTR transposable element (TE).ResultsThe conventional analysis detected 3 individuals (among 50 analyzed) carrying one small metacentric and mitotically unstable B chromosome. The FISH analysis revealed a pericentromeric block of C0t-1 DNA in the B chromosome but no 18S or 5S rDNA clusters in this extra element. Using the LOA-like TE probe, the FISH analysis revealed large pericentromeric blocks in eight autosomal bivalents and in the B chromosome, and a pericentromeric block extending to the short arm in one autosomal pair. No positive hybridization signal was observed for the LOA-like element in the sex chromosomes.ConclusionsThe results indicate that the origin of the B chromosome is associated with the autosomal elements, as demonstrated by the hybridization with C0t-1 DNA and the LOA-like TE. The present study is the first report on the cytogenetic mapping of a TE in coleopteran chromosomes. These TEs could have been involved in the origin and evolution of the B chromosome in C. cyanescens.

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High levels of chromosomal differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae)

Cited by 9 publications

References 25 publications

Cytogenetics of the darkling beetles Zophobas aff. confusus and Nyctobates gigas (Coleoptera, Tenebrionidae)

Cytogenetics of the darkling beetles Zophobas aff. confusus and Nyctobates gigas (Coleoptera, Tenebrionidae)

Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

B chromosome in the beetle Coprophanaeus cyanescens (Scarabaeidae): emphasis in the organization of repetitive DNA sequences

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