High Levels of Antibiotic Resistance but No Antibiotic Production Detected Along a Gypsum Gradient in Great Onyx Cave, KY, USA

Lavoie, Kathleen H.; Ruhumbika, Tania; Bawa, Anissa; Whitney, Aaryn; Ondarza, José de

doi:10.3390/d9040042

Cited by 6 publications

(1 citation statement)

References 21 publications

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“…In general, 80% of bacteria tested are resistant to at least two antimicrobials (Figure 2), showing a pattern of resistance like that observed by Çandiroğl & Güngö (2020) in the Parsik cave in Turkey. Both caves in our study showed high resistance to Trimethoprim, similar to what was observed by Bhullar et al (2012) in 60% of bacteria isolated from the Lechuguilla cave, New Mexico and in isolates from Onyx caves in the United States (LAVOIE, 2017).…”

Section: Assessment Of Antimicrobial Resistancesupporting

confidence: 88%

Prospecting and evaluating antimicrobial resistance and metal tolerance of microorganisms isolated from cave soil in Serra de Carajás – PA

De Souza,

Zonin,

De Souza

et al. 2023

CLCS

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The microbial communities present in cavities, especially in iron lithology environments, remain largely unknown. The isolation and characterization of cave microbiota are essential for identifying new species and obtaining substances of biotechnological relevance, such as antimicrobial compounds and microorganisms capable of bioremediating heavy metals. The objective of this study was to evaluate the antimicrobial activity and metal tolerance of bacteria isolated from the soil of caves GEM-1423 and GEM-1462 located in the Campos Ferruginosos National Park, in Serra de Carajás, Pará, Brazil. The bacteria were isolated from soil in different zones of the caves (photic, penumbra and aphotic) and subjected to antimicrobial susceptibility tests using the disk-diffusion technique and assessment of tolerance to metals by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (CBM). Regarding the resistance profile, the bacteria showed high resistance to the antimicrobials Trimethoprim, Ampicillin and Amoxicillin. All bacteria showed tolerance to the metals Fe, Mn, Pb, Mg and K. These data are fundamental to understanding the potential of these microbial communities in iron lithology environments, thus contributing to the advancement of knowledge around cave microbiology, with implications significant for environmental biotechnology and conservation of underground ecosystems.

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Section: Assessment Of Antimicrobial Resistancesupporting

confidence: 88%

Prospecting and evaluating antimicrobial resistance and metal tolerance of microorganisms isolated from cave soil in Serra de Carajás – PA

De Souza,

Zonin,

De Souza

et al. 2023

CLCS

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Bacterial diversity and geomicrobiology of Winter Wonderland ice cave, Utah, USA

Seixas,

Munroe,

Eggleston

2024

MicrobiologyOpen

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The Winter Wonderland ice cave, located at an elevation of 3140 m above sea level in the Uinta Mountains of northern Utah, USA, maintains a constant sub‐zero temperature. Seasonal snowmelt and rain enter the cave, freeze on the surface of the existing ice, and contribute to a 3‐m‐thick layered ice mass. This ice mass contains organic matter and cryogenic cave carbonates (CCCs) that date back centuries. In this study, samples of ice, liquid water, and exposed CCCs were collected to examine the bacterial communities within the cave and to determine if these communities vary spatially and between sample types. Flow cytometry showed that cell counts are an order of magnitude higher in liquid water samples than in ice. Epifluorescence microscopy and scanning electron microscopy imaging revealed potential coccoid and bacillus microbial morphologies in water samples and putative cells or calcite spherules in the CCCs. The diversity of bacteria associated with soil, identified through sequence‐based analysis, supports the hypothesis that water enters the cave by filtering through soil and bedrock. A differential abundance of bacterial taxa was observed between sample types, with the greatest diversity found in CCCs. This supports a geomicrobiological framework where microbes aggregate in the water, sink into a concentrated layer, and precipitate out of the ice with the CCCs, thereby reducing the cell counts in the ice. These CCCs may provide essential nutrients for the bacteria or could themselves be products of biomineralization.

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Diversity of Polyketide Synthases and Nonribosomal Peptide Synthetases Revealed Through Metagenomic Analysis of a Deep Oligotrophic Cave

2020

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High Levels of Antibiotic Resistance but No Antibiotic Production Detected Along a Gypsum Gradient in Great Onyx Cave, KY, USA

Cited by 6 publications

References 21 publications

Prospecting and evaluating antimicrobial resistance and metal tolerance of microorganisms isolated from cave soil in Serra de Carajás – PA

Prospecting and evaluating antimicrobial resistance and metal tolerance of microorganisms isolated from cave soil in Serra de Carajás – PA

Bacterial diversity and geomicrobiology of Winter Wonderland ice cave, Utah, USA

Diversity of Polyketide Synthases and Nonribosomal Peptide Synthetases Revealed Through Metagenomic Analysis of a Deep Oligotrophic Cave

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