High Levels of Anti‐Mitochondrial Antibodies in MRL Mice Influence of Anti‐DNA Antibodies on Anti‐Mitochondrial Antibodies Measured by an Enzyme‐Linked Immunosorbent Assay

Ohshio, Gakuji; Furukawa, Fukumi; Yoshioka, Hideyuki; Taki, Yoshiro; Manabe, Tadao; Ozawa, Kazue; Tobe, Takayoshi; Hamashima, Yoshihiro

doi:10.1111/j.1348-0421.1987.tb03069.x

Cited by 5 publications

(1 citation statement)

References 15 publications

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“…Enzyme-linked immunosorbent assays (ELISA) for anti-single stranded (ss) DNA antibody and antidouble stranded (ds) DNA antibody were carried out according to the method of Kavai et al (8), with the minor modifications described previously (9). IgG isotypes of these antibodies were determined.…”

Section: Measurements Ofserological Autoimmune Traitsmentioning

confidence: 99%

Evaluation of the Japanese‐Chinese Herbal Medicine, Kampo, for the Treatment of Lupus Dermatoses in Autoimmune Prone MRL/Mp‐lpr/lpr Mice

Kanauchi

Imamura

Takigawa

et al. 1994

The Journal of Dermatology

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Kampo, a Japanese-Chinese traditional herbal medicine, has been used for the treatment of various diseases for about 3,000 years in China. Among herbal medicines, Sairei-to is well known for improving the symptoms of rheumatoid arthritis (RA) and other collagen diseases. However, its immunosuppressive effects on autoimmune cutaneous phenomena are not completely understood. We investigated the effects of Sairei-to on the development of lupus dermatoses in autoimmune-prone MRL/Mp-lpr/lpr (MRL/lpr) mice, an animal model which spontaneously develops skin lesions similar to those seen in human lupus erythematosus. Virgin female MRL/lpr mice at 1 month of age, which were treated orally with Sairei-to, had reduced amounts of IgG deposition at the dermoepidermal junction, titers of anti-DNA antibodies and rheumatoid factor, and lymphoproliferation. These results support the use of traditional herbal medicines in patients with human RA and systemic lupus erythematosus.

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Section: Measurements Ofserological Autoimmune Traitsmentioning

confidence: 99%

Evaluation of the Japanese‐Chinese Herbal Medicine, Kampo, for the Treatment of Lupus Dermatoses in Autoimmune Prone MRL/Mp‐lpr/lpr Mice

Kanauchi

Imamura

Takigawa

et al. 1994

The Journal of Dermatology

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Pathogenesis of lupus dermatoses in autoimmune mice. XIX. Attempts to induce subepidermal immunoglobulin deposition in MRL/Mp-+/+ mice

1993

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The deposition of immunoglobulin (Ig) at the dermo-epidermal junction (DEJ) of the skin, frequently observed in autoimmune mouse strains, is similar to that seen in patients with systemic lupus erythematosus (SLE). MRL/Mp-lpr/lpr (MRL/lpr) mice have an autosomal recessive mutant gene, lpr, which produces massive T-cell proliferation and accelerates the onset of autoimmune diseases. MRL/Mp- +/+ (MRL/n) mice lack the lpr gene, and do not develop autoimmune disease during the first year after birth under pathogen-free conditions. To verify the mechanisms of subepidermal Ig deposition in the skin of LE, we designed an experiment in which we could induce Ig deposition in the control MRL/n mice. Intraperitoneal injection of lymphoproliferative cells of aged MRL/lpr mice induced splenomegaly and splenic granulomatous angitis in the control MRL/n mice. Lipopolysaccharide (LPS), a polyclonal B-cell activator, induced slight splenomegaly and relatively high levels of serum Ig. Dermatopathological investigation revealed mild lymphocyte infiltration without positive Ig deposition at the DEJ of MRL/n mice treated with proliferative T cells. Injection of both proliferative T cells and LPS induced 50% positivity of subepidermal Ig deposition, and high levels of serum immunoglobulins and anti-double stranded DNA (anti-dsDNA) antibodies. These changes were not observed in MRL/n mice injected with thymocytes of newborn MRL/lpr mice. Skin lesions and lupus nephritis were not demonstrated in any of the mice tested. This study suggest that both the mild inflammatory reaction and the presence of anti-dsDNA antibodies are required for the induction Ig deposition at the DEJ in the skin of LE patients.

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FK506: therapeutic effects on lupus dermatoses in autoimmune-prone MRL/Mp-lpr/lpr mice

1995

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The effects of FK506, a new immunosuppressive agent, on the development of lupus dermatoses were investigated in the autoimmune-prone MRL/Mp-lpr/lpr (MRL/lpr) mouse, which is an animal model for the spontaneous development of skin lesions similar to those of human lupus erythematosus (LE). FK506 reduced the incidence of skin lesions, lupus nephritis, the titre of serum anti-double-stranded DNA antibodies and the massive T cell proliferation. The incidence and magnitude of IgG deposition at the dermoepidermal junction were not changed. These results suggest that FK506 is a promising immunosuppressive agent for the control of autoimmune skin diseases.

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High Levels of Anti‐Mitochondrial Antibodies in MRL Mice Influence of Anti‐DNA Antibodies on Anti‐Mitochondrial Antibodies Measured by an Enzyme‐Linked Immunosorbent Assay

Cited by 5 publications

References 15 publications

Evaluation of the Japanese‐Chinese Herbal Medicine, Kampo, for the Treatment of Lupus Dermatoses in Autoimmune Prone MRL/Mp‐lpr/lpr Mice

Evaluation of the Japanese‐Chinese Herbal Medicine, Kampo, for the Treatment of Lupus Dermatoses in Autoimmune Prone MRL/Mp‐lpr/lpr Mice

Pathogenesis of lupus dermatoses in autoimmune mice. XIX. Attempts to induce subepidermal immunoglobulin deposition in MRL/Mp-+/+ mice

FK506: therapeutic effects on lupus dermatoses in autoimmune-prone MRL/Mp-lpr/lpr mice

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