“…For viral DNA replication, the intrinsic DNA helicase activity of T antigen (Stahl et al, 1986) is essential to the melting of the double helix at the viral replication origin Jones and Tjian, 1984;Wold et al, 1987;Scheffner et al, 1989a,b;Borowiec et al, 1990) and to the separation of parental DNA strands during the subsequent elongation phase (Stahl et al, 1985;Wiekowski et al, 1987). SV40, as well as polyomavirus, can also lead to the transformation of susceptible cell lines (for reviews see Tooze, 1981;Rigby and Lane, 1983) by integration of the viral genome into the host DNA, most probably promoted by T antigen (Chia and Rigby, 1981;Della Valle et al, 1981;Hacker and Fluck, 1989). T antigen is also involved in the reverse reaction: the excision of integrated viral DNA from host chromosomes (Miller et al, 1984).…”