High level production of the Magnaporthe grisea fructose 1,6-bisphosphate aldolase enzyme in Escherichia coli using a small volume bench-top fermentor

Labbé, Geneviève; Bezaire, Jeremy; Groot, Sarah de; How, Christine; Rasmusson, Tim; Yaeck, Jason; Jervis, Eric; Dmitrienko, Gary I.; Guillemette, J. Guy

doi:10.1016/j.pep.2006.06.020

Cited by 7 publications

(2 citation statements)

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“…pWT has been characterized extensively and exhibits essentially the same stability, enzymatic activity and structure as WT SOD1 [19,22,23]. Recombinant pWT and mutant SOD1s were expressed as described previously using shaker flask cultures [26,27] or fed-batch fermentation [28]. In the expression system used, SOD1 is exported to the periplasm from which it is obtained by osmotic shock for shaker flask cultures, or from the culture supernatant of fermentation cultures using ammonium sulfate (40% w/v) precipitation.…”

Section: Preparation and Purification Of Recombinant Sod1mentioning

confidence: 99%

Spectrophotometric method for simultaneous measurement of zinc and copper in metalloproteins using 4-(2-pyridylazo)resorcinol

Doyle

Naser

Bauman

et al. 2019

Analytical Biochemistry

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Section: Preparation and Purification Of Recombinant Sod1mentioning

confidence: 99%

Spectrophotometric method for simultaneous measurement of zinc and copper in metalloproteins using 4-(2-pyridylazo)resorcinol

Doyle

Naser

Bauman

et al. 2019

Analytical Biochemistry

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“…The mass spectrometry and bioinformatics of spots 17 and 18 resulted in the identification of the fructose-1,6-bisphosphate aldolase (Fba) protein with a molecular mass of 43 KDa and isoelectric points of 6.00 and 6.14, respectively. Fba proteins are enzymes used in glycolysis and gluconeogenesis and are classified into class I and class II [70][71][72]. Class I enzymes are present in plants, animals, algae, and prokaryotes.…”

Section: Discussionmentioning

confidence: 99%

Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis

Chechi

Rotchanapreeda

Paz

et al. 2021

JoF

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Pythiosis, whose etiological agent is the oomycete Pythium insidiosum, is a life-threatening disease that occurs mainly in tropical and subtropical countries, affecting several animal species. It is frequently found in horses in Brazil and humans in Thailand. The disease is difficult to diagnose because the pathogen’s hyphae are often misdiagnosed as mucoromycete fungi in histological sections. Additionally, there is no specific antigen to use for rapid diagnosis, the availability of which could improve the prognosis in different animal species. In this scenario, we investigated which P. insidiosum antigens are recognized by circulating antibodies in horses and humans with pythiosis from Brazil and Thailand, respectively, using 2D immunoblotting followed by mass spectrometry for the identification of antigens. We identified 23 protein spots, 14 recognized by pooled serum from horses and humans. Seven antigens were commonly recognized by both species, such as the heat-shock cognate 70 KDa protein, the heat-shock 70 KDa protein, glucan 1,3-beta-glucosidase, fructose-bisphosphate aldolase, serine/threonine-protein phosphatase, aconitate hydratase, and 14-3-3 protein epsilon. These results demonstrate that there are common antigens recognized by the immune responses of horses and humans, and these antigens may be studied as biomarkers for improving diagnosis and treatment.

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Natural products as sources of new fungicides (IV): Synthesis and biological evaluation of isobutyrophenone analogs as potential inhibitors of class-II fructose-1,6-bisphosphate aldolase

Luong

Dan

et al. 2018

Bioorganic & Medicinal Chemistry

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High level production of the Magnaporthe grisea fructose 1,6-bisphosphate aldolase enzyme in Escherichia coli using a small volume bench-top fermentor

Cited by 7 publications

References 50 publications

Spectrophotometric method for simultaneous measurement of zinc and copper in metalloproteins using 4-(2-pyridylazo)resorcinol

Spectrophotometric method for simultaneous measurement of zinc and copper in metalloproteins using 4-(2-pyridylazo)resorcinol

Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis

Natural products as sources of new fungicides (IV): Synthesis and biological evaluation of isobutyrophenone analogs as potential inhibitors of class-II fructose-1,6-bisphosphate aldolase

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