High-level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum

Ravasi, Pablo; Braia, Mauricio; Eberhardt, Florencia; Elena, Claudia; Cerminati, Sebastián; Peirú, Salvador; Castelli, María Eugenia; Menzella, Hugo G.

doi:10.1016/j.jbiotec.2015.10.018

Cited by 36 publications

(31 citation statements)

References 39 publications

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“…We speculate that CNTs are possibly universal PLC activators, although it still needs to be investigated whether all types of CNTs are PLC activators. This PLC-activating character of c-MWCNTs may have broad use in many industrial fields, including enzymatic degumming of plant oils 28–30 , manufacturing of biodiesels, food, nutraceuticals, detergents, leather, paper, cosmetics and even agriculture 31 . In biomedical fields, PLC and/or its manipulators may have applications in bioremediation 31 , biosensoring 32 , and microenvironmental modeling of tumors through macrophage recruitment 33, 34 .…”

Section: Discussionmentioning

confidence: 99%

Multi-walled carbon nanotubes act as a chemokine and recruit macrophages by activating the PLC/IP3/CRAC channel signaling pathway

Tan

et al. 2017

Sci Rep

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The impact of nanomaterials on immune cells is gaining attention but is not well documented. Here, we report a novel stimulating effect of carboxylated multi-walled carbon nanotubes (c-MWCNTs) on the migration of macrophages and uncover the underlying mechanisms, especially the upstream signaling, using a series of techniques including transwell migration assay, patch clamp, ELISA and confocal microscopy. c-MWCNTs dramatically stimulated the migration of RAW264.7 macrophages when endocytosed, and this effect was abolished by inhibiting phospholipase C (PLC) with U-73122, antagonizing the IP3 receptor with 2-APB, and blocking calcium release-activated calcium (CRAC) channels with SK&F96365. c-MWCNTs directly activated PLC and increased the IP3 level and [Ca2+]i level in RAW264.7 cells, promoted the translocation of the ER-resident stromal interaction molecule 1 (STIM1) towards the membranous calcium release-activated calcium channel modulator 1 (Orai1), and increased CRAC current densities in both RAW264.7 cells and HEK293 cells stably expressing the CRAC channel subunits Orai1 and STIM1. c-MWCNTs also induced dramatic spatial polarization of KCa3.1 channels in the RAW264.7 cells. We conclude that c-MWCNT is an activator of PLC and strongly recruits macrophages via the PLC/IP3/CRAC channel signaling cascade. These novel findings may provide a fundamental basis for the impact of MWCNTs on the immune system.

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Section: Discussionmentioning

confidence: 99%

Multi-walled carbon nanotubes act as a chemokine and recruit macrophages by activating the PLC/IP3/CRAC channel signaling pathway

Tan

et al. 2017

Sci Rep

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“…The Gram‐positive actinobacterium, Corynebacterium glutamicum , represents an attractive expression platform for secretory protein production in industrial biotechnology due to its ability to secrete proteins at the g L −1 range into the extracellular medium (Freudl, ; Ravasi et al, ; Watanabe, Teramoto, Suzuki, Inui, & Yukawa, ; Yim, Choi, et al, ). Furthermore, it is classified as GRAS (generally regarded as safe) organism and is the major producer for food and feed amino acids (Wendisch, ).…”

Section: Introductionmentioning

confidence: 99%

Combinatorial impact of Sec signal peptides from Bacillus subtilis and bioprocess conditions on heterologous cutinase secretion by Corynebacterium glutamicum

Hemmerich

Moch

Jurischka

et al. 2018

Biotech & Bioengineering

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The impact of Sec signal peptides (SPs) from Bacillus subtilis in combination with isopropyl‐β‐ d‐1‐thiogalactopyranoside concentration and feeding profile was investigated for heterologous protein secretion performance by Corynebacterium glutamicum using cutinase as model enzyme. Based on a comprehensive data set of about 150 bench‐scale bioreactor cultivations in fed‐batch mode and choosing the cutinase yield as objective, it was shown that relative secretion performance for bioprocesses remains very similar, irrespective of the applied SP enabling Sec‐mediated cutinase secretion. However, to achieve the maximal absolute cutinase yield, careful adjustment of bioprocess conditions was found to be necessary. A model‐based, two‐step multiple regression approach resembled the collected data in a comprehensive way. The corresponding results suggest that the choice of the heterologous Sec SP and its interaction with the adjusted exponential feeding profile is highly relevant to maximize absolute cutinase yield in this study. For example, the impact of Sec SP is high at low growth rates and low at high growth rates. However, promising Sec SPs could be inferred from less complex batch cultivations. The extensive data were also evaluated in terms of cutinase productivity, highlighting the well‐known trade‐off between yield and productivity in bioprocess development in detail. Conclusively, only the right combination of target protein, Sec SP, and bioprocess conditions is the key to success.

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“…Oils. Oil samples were taken at the exit of any MFB, and the iron content was determined according to Ravasi et al [21].…”

Section: Detection Of Iron In Fats Andmentioning

confidence: 99%

Application of Magnetic Nano-Immobilized Enzyme in Soybean Oil Degumming: Numerical Simulation in a Liquid-Solid MFB

Chen

Wang

et al. 2021

Journal of Food Quality

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Using crude soybean oil (CSO) as fluid and nanomagnetic immobilized phospholipase C (PLC) as fluidizing particles, the Eulerian–Lagrangian fluid-particle two-phase flow model was used to numerically simulate the law of motion of fluidizing particles in the magnetic fluidized bed (MFB). The main parameters were obtained by numerical simulation based on the discrete element method (DEM). The nanomagnetic PLC in the MFB was optimal to the enzymatic reaction by limiting the iteration step size to 3 × 10−5, the boundary condition to 20 × 300 mm, the opening rate to 37.5%, the condition of CSO flow rate to 0.01 m/s, and the magnetic field strength to 0.02T. After 2.0 h of reaction, the amount of residual phosphorus in the oil was 55.73 mg/kg, the content of 1, 2-DAG was 1.42%, and the nanomagnetic enzyme still had 97% relative activity. Hence, these optimal conditions can improve the efficiency and the stability of the nanomagnetic enzymatic reaction.

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High-level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum

Cited by 36 publications

References 39 publications

Multi-walled carbon nanotubes act as a chemokine and recruit macrophages by activating the PLC/IP3/CRAC channel signaling pathway

Multi-walled carbon nanotubes act as a chemokine and recruit macrophages by activating the PLC/IP3/CRAC channel signaling pathway

Combinatorial impact of Sec signal peptides from Bacillus subtilis and bioprocess conditions on heterologous cutinase secretion by Corynebacterium glutamicum

Application of Magnetic Nano-Immobilized Enzyme in Soybean Oil Degumming: Numerical Simulation in a Liquid-Solid MFB

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