High level of heterozygous haplotype of hemoglobin in Abidjan population with mild malaria

Abstract

Background Sickle cell disease (SCD) is a hemoglobin disorders that concern 300,000 newborns each year around the world. There are hemoglobin haplotypes that affect SCD clinic expression. Methods Our goal was to identify the hemoglobin’s haplotypes among individuals with mild malaria independently of SCD status in Côte d’Ivoire. To determine these haplotypes, specific restriction enzyme (RE) is used after PCR amplification with each primer. According to the digestion of PCR product by RE, five hemoglobin’s haplotypes are found in the world. Results In Côte d’Ivoire, no study has yet deeply described the distribution of haplotypes. Four different “classical” haplotypes of hemoglobin were detected: Benin (56.5%), Bantou (28.5%), Senegal (4%), Cameroun (1%); and 10% of atypical profiles. Heterozygous haplotype (69%) were more frequent than homozygous haplotype (31%). Conclusions In this preliminary study, we note a high prevalence of atypical and heterozygous haplotype. Benin haplotype that is associated with severity of SCD was most predominant in our studied population.

Influence of Hemoglobin S Haplotypes on the Responses to Hydroxyurea Treatment in Children with Sickle Cell Disease in Abidjan, Côte d’Ivoire

“…One with ethylene diamine tetra-acetate (EDTA) as anticoagulant were drawn from fasting children for at least 10 hours at the inclusion and 12 months into HU treatment. The first part of the EDTA sample was used for the hematological assays on the automated blood cells counter XT of Sysmex, and for alkaline and acid electrophoreses of hemoglobin [20]. And the last part of it was used to perform a PCR-RFLP method that used five different primer sets amplifying specific regions within the human β gene cluster (Gγ, Aγ, the first and second sites inside Ψβ, δ), and three restriction enzymes that were HindIII, HindII and HincI [6].…”