High level of drug resistance by gram-negative bacteria from selected sewage polluted urban rivers in Addis Ababa, Ethiopia

Belachew, Teshome; Mihret, Adane; Legesse, Tesfaye Girma; Million, Yihenew; Desta, Kassu

doi:10.1186/s13104-018-3622-0

Cited by 22 publications

(36 citation statements)

References 19 publications

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“…On the contrary, resistance to trimethoprim/sulfamethoxazole (57.1%), gentamicin (28.6%) and ciprofloxacin (14.3%) in our study was lower than Belachew et al [17] showing 67%, 43%, and 52% respectively. It has been observed that none of the hospitals in Biratnagar have waste treatment system as a result, 85.7% of E. coli species had multi-drug resistance, which is, higher compared to previously reported results in Ethiopia (78%) [17] and Romania (60.34%) [18]. Such a high resistance rate may be a result of poor waste management practice, lack of treatment plants for healthcare institutions and poor antimicrobial usage in Biratnagar.…”

Section: Discussioncontrasting

confidence: 88%

“…Similarly, the resistance to cefoxitin, cefuroxime, cefpodoxime, and nitrofurantoin in this study was found to be much higher than other studies [17]. A high rate of MDR (66.7%) was observed for Klebsiella spp which was higher than results reported in Ethiopia (40.5%) [17], Romania (33%) [18] and Mexico (50%) [19]. However, MDR rate of the current finding was lower than previously reported results in Brazil (77.5%) [20].…”

Section: Discussionsupporting

confidence: 51%

“…Resistance shown by Trimethoprim/sulfamethoxazole (50%), chloramphenicol (33.3%) and ciprofloxacin (33.3%) were much higher than found in Romania as 22.4%, 5.2% and 6.9%. Similarly, the resistance to cefoxitin, cefuroxime, cefpodoxime, and nitrofurantoin in this study was found to be much higher than other studies [17]. A high rate of MDR (66.7%) was observed for Klebsiella spp which was higher than results reported in Ethiopia (40.5%) [17], Romania (33%) [18] and Mexico (50%) [19].…”

Section: Discussioncontrasting

confidence: 46%

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Detection of extended-spectrum beta-lactamase-producing E. coli and Klebsiella spp in effluents of different hospitals sewage in Biratnagar, Nepal

Mahato¹,

Mahato²,

Pokharel

et al. 2019

Preprint

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Objective This study was aimed to determine prevalence and resistance pattern like multidrug resistant (MDR) or ESBL nature of E. coli and Klebsiella spp from various sewage drain samples with an idea to deliver baseline information that could be utilized for defining guidelines for the treatment of hospital sewages.Results Of 10 sewage samples analyzed, 7 (70%) contained E. coli while 6 (60%) contained Klebsiella. Except one sample, all positive samples contained both E. coli and Klebsiella spp. E. coli isolates were resistant to ampicillin, amoxicillin, cefoxitin, cefuroxime, and cefpodoxime; while 85.7% were resistant to amoxicillin/clavulanate, ceftazidime, cefotaxime and ceftriaxone. 71.4%, 57.1%, 42.9%, and 28.6% were resistant to aztreonam, trimethoprim/sulfamethoxazole, nitrofurantoin, and gentamicin. Most were sensitive to chloramphenicol, ofloxacin, ciprofloxacin, and azithromycin. 85.7% and 57.1% of E. coli were MDR and ESBL isolates respectively. Klebsiella were resistant to ampicillin, amoxicillin, and amoxicillin/clavulanate. 83.4% of Klebsiella were resistant to cefoxitin. 66.7% of strains were resistant to cefuroxime, ceftazidime, cefotaxime, ceftriaxone, and cefpodoxime. Klebsiella showed 50% resistant to aztreonam and trimethoprim/sulfamethoxazole, while 33.3% were resistant to chloramphenicol, nitrofurantoin, ofloxacin, and ciprofloxacin. Klebsiella were sensitive to azithromycin and gentamicin. 66.7% and 33.3% of Klebsiella were MDR and ESBL isolates respectively.

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Section: Discussioncontrasting

confidence: 88%

Section: Discussionsupporting

confidence: 51%

Section: Discussioncontrasting

confidence: 46%

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Detection of extended-spectrum beta-lactamase-producing E. coli and Klebsiella spp in effluents of different hospitals sewage in Biratnagar, Nepal

Mahato¹,

Mahato²,

Pokharel

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Klebsiella pneumoniae showed 100% resistance to ampicillin and amoxicillin/clavulanatewhich was higher than the study of Ethiopia (94%) [17] and Romania (70.7%) [18]. Resistance to penicillin antibiotics like ampicillin has become very common in the world and our finding is in line with this evidence.…”

Section: Discussionsupporting

confidence: 88%

Detection of extended-spectrum beta-lactamase-producing E. coli and Klebsiella spp in effluents of different hospitals sewage in Biratnagar, Nepal

Mahato¹,

Mahato²,

Pokharel

et al. 2019

Preprint

View full text Add to dashboard Cite

ObjectiveThis study was aimed to determine prevalence and resistance pattern like multidrug resistant (MDR) or ESBL nature of E. coli and Klebsiella spp from various sewage drain samples with an idea to deliver baseline information that could be utilized for defining guidelines for the treatment of hospital sewages.ResultsOf 10 sewage samples analyzed, 7 (70%) contained E. coli while 6 (60%) contained Klebsiella. Except one sample, all positive samples contained both E. coli and Klebsiella spp. E. coli isolates were resistant to ampicillin, amoxicillin, cefoxitin, cefuroxime, and cefpodoxime; while 85.7% were resistant to amoxicillin/clavulanate, ceftazidime, cefotaxime and ceftriaxone. 71.4%, 57.1%, 42.9%, and 28.6% were resistant to aztreonam, trimethoprim/sulfamethoxazole, nitrofurantoin, and gentamicin. Most were sensitive to chloramphenicol, ofloxacin, ciprofloxacin, and azithromycin. 85.7% and 57.1% of E. coli were MDR and ESBL isolates respectively. Klebsiella were resistant to ampicillin, amoxicillin, and amoxicillin/clavulanate. 83.4% of Klebsiella were resistant to cefoxitin. 66.7% of strains were resistant to cefuroxime, ceftazidime, cefotaxime, ceftriaxone, and cefpodoxime. Klebsiella showed 50% resistant to aztreonam and trimethoprim/sulfamethoxazole, while 33.3% were resistant to chloramphenicol, nitrofurantoin, ofloxacin, and ciprofloxacin. Klebsiella were sensitive to azithromycin and gentamicin. 66.7% and 33.3% of Klebsiella were MDR and ESBL isolates respectively.

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“…Best environmental conditions favor the selective pressure and greater amount of biogeochemical compounds tend to increase in total biomass and reduced diversity due to the high competition for resources, prevailing with some groups over others, thus contributing to these environmental adaptations through genetic mechanisms elevating the spread of genes that favor the microorganisms resistant to the different classes of antimicrobial agents [28]. This shows that biological diversity is present at the point after the sewage releases, which was expected due to the presence of high load organic from the effluent, since the organic discharge promotes biodiversity and increases the diversity of metabolic profiles [29]. For the richness of the substrate (S) for sample 1 (one) equal to 44 (forty-four), and sample 2 of 65 (sixty-five), where S reflects the number of microorganisms found in the environment, there is a greater amount of microorganisms present in two points where there is a greater insertion effluent [28].…”

Section: Resultsmentioning

confidence: 99%

Detection of Gram-Negative Bacteria Carrying the blaKPC-2 Gene from Mangrove Sediments

Neto¹,

Araújo²,

Silva³

et al. 2019

JPP

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Mangroves are environments that have fast cycles associated with high concentration of bacterial decomposers. These are impacted by anthropogenic pollution due to contamination of bacterial species carrying resistance genes. This study aims to evaluate the metabolic profile of the microbiota in mangrove sediments, and verify the presence of Gram-negative bacteria resistant to meropenem. The samples were obtained from location along the Anil river and were seeded in medium supplemented with the antibiotic meropenem in increasing concentrations. The DNA was analyzed by multiplex PCR for detecting resistance genes for β-lactam antibiotics. The bacteria were identified by Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The genes were sequenced by ABI PRISM 3100, analyzed by MEGA 6.0 program, and the sequence identified was assessed by GenBank using the BLAST algorithm. Ecoplate® the kit was used to determine the metabolic profile of the microbiota. The results of the six bacterial isolates showed the bla KPC-2 gene and were identified as Stenotrophomonas maltophilia and Pseudomonas putida. The samples showed a greater diversity Shannon index, a rich substrate consumption and high equity. There was a metabolic richness such as carbon consumption profiles, being a factor of adaptation of pathogenic bacteria carrying resistance genes to β-lactamics antibiotics.

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High level of drug resistance by gram-negative bacteria from selected sewage polluted urban rivers in Addis Ababa, Ethiopia

Cited by 22 publications

References 19 publications

Detection of extended-spectrum beta-lactamase-producing E. coli and Klebsiella spp in effluents of different hospitals sewage in Biratnagar, Nepal

Detection of extended-spectrum beta-lactamase-producing E. coli and Klebsiella spp in effluents of different hospitals sewage in Biratnagar, Nepal

Detection of extended-spectrum beta-lactamase-producing E. coli and Klebsiella spp in effluents of different hospitals sewage in Biratnagar, Nepal

Detection of Gram-Negative Bacteria Carrying the blaKPC-2 Gene from Mangrove Sediments

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