High‐level expression of the ER‐MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage

Bruijn, Marella F. T. R. de; Ploemacher, Rob E.; Mayen, Angelique E. M.; Voerman, Jane S. A.; Slieker, W. A. T.; Ewijk, Willem van; Leenen, Pieter J. M.

doi:10.1002/eji.1830261208

Cited by 27 publications

(20 citation statements)

References 27 publications

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“…Indeed, the local pancreas DC precursor has a similar phenotype as blood monocytes, except for the lower CD11c expression on the Ly6C low cells and is expressing ER-MP58, which is a marker for both myeloid precursors in the BM and peripheral blood monocytes [15]. Upon GM-CSF stimulation the local ER-MP58 1 cells isolated from fetal pancreas displayed a high proliferative activity.…”

Section: Discussionmentioning

confidence: 99%

“…However, some non-myeloid cells can express this marker at low levels [15]. Isolated ER-MP58 1 cells from the pancreas were used in cultures with GM-CSF and developed into DCs.…”

Section: Discussionmentioning

confidence: 99%

“…ER-MP58 has previously been described by our laboratory as a marker for all myeloid progenitor cells in BM [15]. A double staining with ER-MP58 and insulin was performed on the E15.5 pancreas of C57BL/6 and NOD/LTj mice using immunofluorescence (Fig.…”

Section: Myeloid Precursor Populations In the Fetal Pancreasmentioning

confidence: 99%

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Abnormalities of dendritic cell precursors in the pancreas of the NOD mouse model of diabetes

et al. 2011

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The non-obese diabetic (NOD) mouse is a widely used animal model for the study of human diabetes. Before the start of lymphocytic insulitis, DC accumulation around islets of Langerhans is a hallmark for autoimmune diabetes development in this model. Previous experiments indicated that an inflammatory influx of these DCs in the pancreas is less plausible. Here, we investigated whether the pancreas contains DC precursors and whether these precursors contribute to DC accumulation in the NOD pancreas. Fetal pancreases of NOD and control mice were isolated followed by FACS using ER-MP58, Ly6G, CD11b and Ly6C. Sorted fetal pancreatic ER-MP58 1 cells were cultured with GM-CSF and tested for DC markers and antigen processing. CFSE labeling and Ki-67 staining were used to determine cell proliferation in cultures and tissues. Ly6C hi and Ly6C low precursors were present in fetal pancreases of NOD and control mice. These precursors developed into CD11c 1 MHCII 1 CD86 1 DCs capable of processing DQ-OVA. ER-MP58 1 cells in the embryonic and pre-diabetic NOD pancreas had a higher proliferation capacity. Our observations support a novel concept that pre-diabetic DC accumulation in the NOD pancreas is due to aberrant enhanced proliferation of local precursors, rather than to aberrant ''inflammatory infiltration'' from the circulation. IntroductionThe non-obese diabetic (NOD) mouse is used as a spontaneous model to study the development of type 1 diabetes [1]. Lymphocytes accumulate around and in the islets of Langerhans in NOD mice from around 6 weeks of age onwards, which results in the destruction of b-cells followed by a decrease in insulin production leading to diabetes. Prior to T-and B-cell accumulation the number of DCs increases in the pancreas and concentrates around the islets (from the age of 5 weeks onwards) [2,3]. DCs are potent APCs capable of stimulating both naïve and memory T cells [4]. The observation that DCs are the first immune cells to increase in number in the NOD pancreas points to a crucial role for DCs in the initiation of the islet autoimmune reaction. Such a role was recently proven by the demonstration that a temporal depletion of DCs totally abrogated the development of insulitis and diabetes in the NOD mouse model [5].Early studies have shown that BM precursors give rise to monocytes in blood, which circulate for a few days before they migrate into tissue where they develop into different types of DCs and macrophages. Blood monocytes can be subdivided into at least two subsets based on their Ly6C expression: classical and The nonclassical monocytes, which are Ly6C low , patrol the endothelium of the blood vessels and are required for rapid tissue invasion at the site of an infection [7]. Ly6C low monocytes are considered CD11c À , but some studies have reported the expression of CD11c on these cells [6,8]. Both types of monocytes are F4/80 1 and CD86 À [6].Data are accumulating on the presence of local tissue precursors for DCs and macrophages and the contribution of these precursors to DC and ma...

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Section: Discussionmentioning

confidence: 99%

“…However, some non-myeloid cells can express this marker at low levels [15]. Isolated ER-MP58 1 cells from the pancreas were used in cultures with GM-CSF and developed into DCs.…”

Section: Discussionmentioning

confidence: 99%

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Abnormalities of dendritic cell precursors in the pancreas of the NOD mouse model of diabetes

et al. 2011

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“…We also measured the expression of the hematopoietic markers ER-MP12, ER-MP20, and ER-MP58, which recognize hematopoietic precursors and have previously been used as markers of monocytic differentiation. [29][30][31] We could detect very low For personal use only. on May 10, 2018. by guest www.bloodjournal.org From levels of ER-MP12, but not ER-MP20 or ER-MP58, on 13␣ Ϫ/Ϫ cells, consistent with an early myeloid phenotype (data not shown).…”

Section: Establishment Of Early Myeloid Cell Lines From C/ebp␣ ؊/؊ Fementioning

confidence: 99%

Induction of granulocytic differentiation by 2 pathways

Zhang

Nelson

Radomska

et al. 2002

Blood

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“…However, heterogeneity of monocyte populations has been poorly understood, particularly in comparison with lymphoid and dendritic cell populations. Several monocytic/myeloid precursor populations in BM have been defined by cytochemical methods (2)(3)(4)(5) as well as antigen expression (5)(6)(7)(8)(9)(10), and recent studies have begun to define subpopulations of monocytes in murine peripheral blood (PB) (11)(12)(13). These populations have been defined by differential expression of several markers, such as CX 3 (12,13).…”

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confidence: 99%

Phenotypic comparison of multiple monocyte‐related populations in murine peripheral blood and bone marrow

Schlueter

Glasgow

2006

Cytometry Pt A

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Background: Monocyte subsets are not well defined in murine peripheral blood (PB). Monocyte-related populations could also be located in bone marrow (BM), but studies correlating monocyte populations found in these two tissues are lacking. This study simultaneously analyzed PB and BM to phenotypically define multiple monocyterelated subsets in each location. Methods: Murine PB and BM cells were simultaneously stained for monocyte-related populations, using five-color flow cytometry. Relevant subsets were defined on the basis of Ly-6C, CD11b, and wheat germ agglutinin phenotype in addition to light-scatter characteristics. These populations were extensively characterized for the expression of other myeloid and dendritic cell markers, adhesion molecules, chemokine receptors, and growth factor receptors. Results: Six monocyte-related populations were defined,

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High‐level expression of the ER‐MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage

Cited by 27 publications

References 27 publications

Abnormalities of dendritic cell precursors in the pancreas of the NOD mouse model of diabetes

Abnormalities of dendritic cell precursors in the pancreas of the NOD mouse model of diabetes

Induction of granulocytic differentiation by 2 pathways

Phenotypic comparison of multiple monocyte‐related populations in murine peripheral blood and bone marrow

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