High-level expression of SAG1 and GRA7 gene of Toxoplasma gondii (Izatnagar isolate) and their application in serodiagnosis of goat toxoplasmosis

Velmurugan, G. V.; Tewari, A. K.; Rao, J. R.; Baidya, Surajit; Kumar, Manchukonda Udaya; Mishra, A. K.

doi:10.1016/j.vetpar.2008.03.032

Cited by 43 publications

(25 citation statements)

References 14 publications

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“…For years, the serological profile defined by experienced investigators has been accepted in laboratory diagnostic routine. However, current laboratory practice using highly sensitive automated equipment has led to problems with the detection of parasite specific IgG and IgM antibodies 12,33 . Several authors have shown that IgM to T. gondii can be detected for a long period after the primary infection and false positivities with IgG to T. gondii have been reported which are non-significant and erroneously interpreted as positive results indicating acute infection and protection 19 .…”

Section: Discussionmentioning

confidence: 99%

High diagnostic efficiency of IgM-ELISA with the use of multiple antigen peptides (MAP1) from T. gondii ESA (SAG-1, GRA-1 and GRA-7), in acute toxoplasmosis

Araújo

Ferreira

2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe main serological marker for the diagnosis of recent toxoplasmosis is the specific IgM antibody, along with IgG antibodies of low avidity. However, in some patients these antibodies may persist long after the acute/recent phase, contributing to misdiagnosis in suspected cases of toxoplasmosis. In the present study, the diagnostic efficiency of ELISA was evaluated, with the use of peptides derived from T. gondii ESA antigens, named SAG-1, GRA-1 and GRA-7. In the assay referred to, we studied each of these peptides individually, as well as in four different combinations, as Multiple Antigen Peptides (MAP), aiming to establish a reliable profile for the acute/recent toxoplasmosis with only one patient serum sample. The diagnostic performance of the assay using MAP1, with the combination of SAG-1, GRA-1 and GRA-7 peptides, demonstrated better discrimination of the acute/recent phase from non acute/ recent phase of toxoplasmosis. Our results show that IgM antibodies to MAP1 may be useful as a serological marker, enhancing the diagnostic efficiency of the assay for acute/recent phase of toxoplasmosis.

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Section: Discussionmentioning

confidence: 99%

High diagnostic efficiency of IgM-ELISA with the use of multiple antigen peptides (MAP1) from T. gondii ESA (SAG-1, GRA-1 and GRA-7), in acute toxoplasmosis

Araújo

Ferreira

2010

Rev. Inst. Med. trop. S. Paulo

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“…Shaapan et al (2008) reported a diagnostic sensitivity of 96.2% and a specificity of 88.9% for this test when compared with the gold standard test such as SabinFeldman dye test (Sukthana et al 2001). It is, however, recommended that further comparative studies should be carried out using tests with higher sensitivities and specificities such as fluorogenic probe and real-time PCR (Brandão et al 2006;Velmurugan et al 2008;Bontell et al 2009 Na not applicable; OR Odd ratio Reports for toxoplasmosis in other livestock specie in Africa and other tropical regions revealed higher prevalence in sheep and goats, as exemplified by 31% in goats in Uganda (Bisson et al 2000), 29.4% sheep in Brazil(Clementino et al 2007), 23.4% in sheep and19.3% in goats in Saudi Arabia (Sanad and Al-Ghabban 2007), 43% in sheep in Egypt (Shaapan et al 2008), 52.6% in sheep and 74% in goats in Ethiopia (Teshale et al 2007), but also in pigs, where Hove et al (2005) reported a prevalence of 23% in Zimbabwe. Prevalence seems to be higher in sheep and goats in countries with a wet climate than in countries with a hot and dry climate (Samra et al 2007;Shaapan et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Sero-epidemiological investigation of bovine toxoplasmosis in traditional and smallholder cattle production systems of Tanga Region, Tanzania

Schoonman

Wilsmore

Swai³

2009

Trop Anim Health Prod

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In view of the worldwide importance of Toxoplasma gondii and the fragmented information on the seroprevalence of the disease in animals in Tanzania, a study, using the modified Eiken latex agglutination test (LAT), was conducted from May 2003 to January 2004 to determine the prevalence of antibody to T. gondii in 130 randomly selected farms comprising 655 cattle. The overall seroprevalence of T.gondii antibodies in cattle and farms were 3.6% and 13%, respectively. Risk factors for animal and herd-level toxoplasmosis seropositivity were tested using multivariable logistic regression to control for confounding factors. Cattle managed under traditional husbandry practises were more likely to be seropositive than those managed under smallholder practises (48% versus 4.7%; p < 0.01). Herd size of > or = 9 cattle were at greater risk of acquiring infection than herds holding fewer animals [< or = 9 cattle], (odd ratio [OR] = 3.99; 95% confidence interval [CI], 0.97-16.48; P = 0.001). We concluded that seroprevalence at herd level was high and relatively low at animal level, possibly due to the reduced susceptibility of cattle to T.gondii infection as compared to goats and sheep. The high seroprevalence in animals managed by traditional husbandry practise suggests that the parasite is widely distributed in the environment and could pose a public health threat to the people living in those areas.

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“…Therefore, the need for developing a protective recombinant protein based vaccines has been emphasized (Jenkins 2001;Vermeulen 1998). As a matter of fact several target apicomplexan proteins have been expressed and used for either diagnostic or prophylactic purposes (Velmurugan et al 2008;Tewari et al 2007Tewari et al , 2010. Microneme proteins are common to apicomplexan parasites and are involved in host cell adhesion and penetration.…”

Section: Recombinant Vaccinementioning

confidence: 99%

Control of poultry coccidiosis: changing trends

Tewari

Maharana

2011

J Parasit Dis

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Coccidiosis is the most important protozoan disease affecting the poultry industry worldwide. Control of poultry coccidiosis is presently based on managerial skills and the use of prophylactic coccidiostatic drugs. With the emergence of drug resistant Eimeria strains, emphasis has been laid on development and use of safer vaccines; some of them have been commercialized successfully. The present review deals with the various factors responsible for the development of clinical coccidiosis in poultry as well as an overview of the currently available inducers and boosters of immunity against coccidiosis. There are three groups of vaccines currently available against coccidiosis which can be distinguished on the basis of characteristics of the Eimeria species included in the respective products, viz. vaccines based on live virulent strains, vaccines based on live attenuated strains, and vaccines based on live strains that are relatively tolerant to the ionophore compounds. The latter vaccine combines the early chemotherapeutic effect of ionophores with the late prophylactic effect of vaccination. Although in the near future more varieties of oocyst based live vaccines are expected, identification of selective coccidian-specific immunoprotective molecules is likely to get more attention to facilitate the sustainable control of poultry coccidiosis.

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High-level expression of SAG1 and GRA7 gene of Toxoplasma gondii (Izatnagar isolate) and their application in serodiagnosis of goat toxoplasmosis

Cited by 43 publications

References 14 publications

High diagnostic efficiency of IgM-ELISA with the use of multiple antigen peptides (MAP1) from T. gondii ESA (SAG-1, GRA-1 and GRA-7), in acute toxoplasmosis

High diagnostic efficiency of IgM-ELISA with the use of multiple antigen peptides (MAP1) from T. gondii ESA (SAG-1, GRA-1 and GRA-7), in acute toxoplasmosis

Sero-epidemiological investigation of bovine toxoplasmosis in traditional and smallholder cattle production systems of Tanga Region, Tanzania

Control of poultry coccidiosis: changing trends

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