2016
DOI: 10.3390/ijms17060902
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High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity

Abstract: In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expression of recombinant bovine lactoferrin (rbLf) in Pichia pastoris. After Bach fermentation and purification by molecular exclusion, we obtained an expression yield of 3.5 g/L of rbLf. rbLf and predominantly pepsin-d… Show more

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Cited by 39 publications
(31 citation statements)
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“…Concerning this, Lf from different origins, mainly from human and bovine, but also from camel, buffalo, and other animals, has been obtained from milk and colostrum. To have a better quality and production of Lf, since 25 years ago Lf has been cloned in different vectors and expressed overall in eukaryotic systems which can glycosylate it, such as yeasts and fungi [154][155][156]. From these organisms, Lf has been highly purified as a recombinant protein and its biological role, mainly antibacterial, has been confirmed.…”
Section: High-scale Production Of Lactoferrinmentioning
confidence: 99%
See 1 more Smart Citation
“…Concerning this, Lf from different origins, mainly from human and bovine, but also from camel, buffalo, and other animals, has been obtained from milk and colostrum. To have a better quality and production of Lf, since 25 years ago Lf has been cloned in different vectors and expressed overall in eukaryotic systems which can glycosylate it, such as yeasts and fungi [154][155][156]. From these organisms, Lf has been highly purified as a recombinant protein and its biological role, mainly antibacterial, has been confirmed.…”
Section: High-scale Production Of Lactoferrinmentioning
confidence: 99%
“…From these organisms, Lf has been highly purified as a recombinant protein and its biological role, mainly antibacterial, has been confirmed. In addition, human Lf has been cloned in transgenic cows and plants [156][157][158][159]. Interestingly, recombinant hLf expressed in cows enhanced systematic and intestinal immune responses in piglets, used as a model of infants [160].…”
Section: High-scale Production Of Lactoferrinmentioning
confidence: 99%
“…It is characterized by its strong regulator promoter, rapid growth and easy genetic manipulation. Moreover, it has the ability to perform eukaryotic post‐translational modifications (Iglesias‐Figueroa et al., ). Given that the production of recombinant IGF‐1 and IGF‐2 is critical for investigating the physiological functions and potential application of these hormones in tongue sole, the aims of the present study were (1) to obtain recombinant proteins of tongue sole IGF‐1 and IGF‐2 expressed in P. pastoris and (2) to examine the comparative activity of IGF‐1 and IGF‐2 in the tongue sole.…”
Section: Introductionmentioning
confidence: 99%
“…Alcohol oxidase 1 promoter (AOX1) is one of the most widely utilized among all the available promoters for P. pastoris 12, 13. It is tightly repressed during yeast growth on glucose or ethanol, but effectively induced by methanol, 14 which, however, is toxic at high levels 15 .…”
Section: Introductionmentioning
confidence: 99%