This study validates the Turkish version of the Attitudes Toward Face Mask Use Scale (ATFMUS). This scale is crucial for understanding public attitudes toward mask usage in preventing the transmission of infectious diseases. The research was conducted between September 2023 and February 2024 with 530 students from a foundation university’s health sciences faculty. The study assessed the scale’s language equivalence, content validity, and factor structure through exploratory and confirmatory factor analyses. Reliability was examined via item-total correlations, Cronbach’s alpha coefficient, and test-retest reliability, indicating satisfactory internal consistency and stability over time. The results confirm the Turkish ATFMUS’s validity and reliability in measuring attitudes toward mask usage. This offers essential insights for formulating preventive initiatives within Turkish communities.